Background:We have shown that downregulation of materally expressed gene 3(MEG3)promoted autophagy and epithelial-mesenchymal transition to promote neuroblastoma(NB)progression.In present study,we aim to further disco...Background:We have shown that downregulation of materally expressed gene 3(MEG3)promoted autophagy and epithelial-mesenchymal transition to promote neuroblastoma(NB)progression.In present study,we aim to further discover the role of MEG3 in NB.Methods:Expression levels of MEG3 in stabled transfected cells were detected by qPCR.Flow cytometry was used to examine the effects of MEG3 overexpression on apoptosis and cell cycle.Moreover,tumor stemness was detected by tumor sphere formation assay.Results:QPCR showed that MEG3 was successfully overexpressed in SK-N-BE(2)C and SK-N-AS cells.CCK8 indicated MEG3 reduced cell proliferation in two NB cells.Flow cytometry revealed that MEG3 promoted apoptosis and caused G0/G1 arrest.Using chromatin isolation by RNA purification(ChIRP)and tumor sphere formation assays,we revealed that upregulation of MEG3 decreased tumor stemness by decreasing Nestin transcription.Conclusions:Overall,present study confirmed an anti-cancer role of MEG3 in NB,demonstrated that MEG3 could be a potential therapeutic target of NB.展开更多
文摘Background:We have shown that downregulation of materally expressed gene 3(MEG3)promoted autophagy and epithelial-mesenchymal transition to promote neuroblastoma(NB)progression.In present study,we aim to further discover the role of MEG3 in NB.Methods:Expression levels of MEG3 in stabled transfected cells were detected by qPCR.Flow cytometry was used to examine the effects of MEG3 overexpression on apoptosis and cell cycle.Moreover,tumor stemness was detected by tumor sphere formation assay.Results:QPCR showed that MEG3 was successfully overexpressed in SK-N-BE(2)C and SK-N-AS cells.CCK8 indicated MEG3 reduced cell proliferation in two NB cells.Flow cytometry revealed that MEG3 promoted apoptosis and caused G0/G1 arrest.Using chromatin isolation by RNA purification(ChIRP)and tumor sphere formation assays,we revealed that upregulation of MEG3 decreased tumor stemness by decreasing Nestin transcription.Conclusions:Overall,present study confirmed an anti-cancer role of MEG3 in NB,demonstrated that MEG3 could be a potential therapeutic target of NB.
