Objective:To explore the efficacy of n-hexane extract of Emilia sonchifolia(E.sonchifobia) against ethanol induced pancreatic dysfunction in the young Wistar albino rats.Methods:The rats were divided into four groups....Objective:To explore the efficacy of n-hexane extract of Emilia sonchifolia(E.sonchifobia) against ethanol induced pancreatic dysfunction in the young Wistar albino rats.Methods:The rats were divided into four groups.Control rats in group I received distilled water orally,groupⅡreceived oral administration of 20%(w/v) ethanol dissolved in drinking water,groupⅢreceived oral administration of 20%(w/v) ethanol in distilled water+n-hexane extract of E.sonchifolia (250 mg/kg body weight),and groupⅣreceived oral administration of n-hexane extract of E.sonchifolia(250 mg/kg body weight) alone.Liver marker enzymes aspartate aminotransferase (AST),alanine aminotransferase(ALT),pancreatic enzymatic antioxidants superoxide dismutase. lipid peroxidation,catalase,glutathione peroxidase,non-enzymatic antioxidants glutathione and vitamin C were measured and compared.Results:Administration of 20%ethanol lor 16 weeks significantly increased the liver marker enzymes AST,ALT(P【0.05).reduced the pancreatic enzymatic antioxidants superoxide dismutase.lipid peroxidation,catalase,glutathione peroxidase,glutathione and vitamin C(P【0.05).Histopathological examination showed that the ethanol provoked the oxidative stress which was demonstrated as pancreatic necrosis and oedema.Simultaneous administration of n-hexane extract of E.sonchifolia(250 mg/kg body weight) protected the pancreas against the damage induced by ethanol which was confirmed by the histopathological studies and the normalization of biochemical parameters.Conclusions: Thus n-hexane extract of E.sonchifolia shows a promise in therapeutic use in alcohol induced oxidative stress.展开更多
文摘Objective:To explore the efficacy of n-hexane extract of Emilia sonchifolia(E.sonchifobia) against ethanol induced pancreatic dysfunction in the young Wistar albino rats.Methods:The rats were divided into four groups.Control rats in group I received distilled water orally,groupⅡreceived oral administration of 20%(w/v) ethanol dissolved in drinking water,groupⅢreceived oral administration of 20%(w/v) ethanol in distilled water+n-hexane extract of E.sonchifolia (250 mg/kg body weight),and groupⅣreceived oral administration of n-hexane extract of E.sonchifolia(250 mg/kg body weight) alone.Liver marker enzymes aspartate aminotransferase (AST),alanine aminotransferase(ALT),pancreatic enzymatic antioxidants superoxide dismutase. lipid peroxidation,catalase,glutathione peroxidase,non-enzymatic antioxidants glutathione and vitamin C were measured and compared.Results:Administration of 20%ethanol lor 16 weeks significantly increased the liver marker enzymes AST,ALT(P【0.05).reduced the pancreatic enzymatic antioxidants superoxide dismutase.lipid peroxidation,catalase,glutathione peroxidase,glutathione and vitamin C(P【0.05).Histopathological examination showed that the ethanol provoked the oxidative stress which was demonstrated as pancreatic necrosis and oedema.Simultaneous administration of n-hexane extract of E.sonchifolia(250 mg/kg body weight) protected the pancreas against the damage induced by ethanol which was confirmed by the histopathological studies and the normalization of biochemical parameters.Conclusions: Thus n-hexane extract of E.sonchifolia shows a promise in therapeutic use in alcohol induced oxidative stress.
