AIM: To search the organelle based changes in hepatocytes after taurine treatment in experimental liver fi brosis induced by CCl_4 administration. METHODS: Thirty rats were divided into two groups. Group 1 (n = 15) wa...AIM: To search the organelle based changes in hepatocytes after taurine treatment in experimental liver fi brosis induced by CCl_4 administration. METHODS: Thirty rats were divided into two groups. Group 1 (n = 15) was injected with CCl_4 plus taurine and Group 2 (n = 15) with CCl_4 plus saline for 12 wk. At the end of 12th wk, mitochondria, rough and smooth endoplasmic reticulum, and nuclei of hepatocytes were evaluated using a scoring system. The results were compared with histopathological f indings, as well. RESULTS: Taurine treatment reduced fi brosis scores signifi cantly as compared to placebo. Organelle injury scores decreased signifi cantly with taurine treatment. Ultrastructural and histopathological scores in both groups were in strong correlation (r = 0.931 for CCl_4 plus taurine and r = 0.899 for CCl_4 plus saline group). CONCLUSION: Organelle based transmission electron microscopy fi ndings can reflect successfully histological results as well as tissue healing in hepatocytes from hepatotoxin-induced liver fi brosis.展开更多
基金Grants from the Gulhane Medical Research Council, No. AR-99/01
文摘AIM: To search the organelle based changes in hepatocytes after taurine treatment in experimental liver fi brosis induced by CCl_4 administration. METHODS: Thirty rats were divided into two groups. Group 1 (n = 15) was injected with CCl_4 plus taurine and Group 2 (n = 15) with CCl_4 plus saline for 12 wk. At the end of 12th wk, mitochondria, rough and smooth endoplasmic reticulum, and nuclei of hepatocytes were evaluated using a scoring system. The results were compared with histopathological f indings, as well. RESULTS: Taurine treatment reduced fi brosis scores signifi cantly as compared to placebo. Organelle injury scores decreased signifi cantly with taurine treatment. Ultrastructural and histopathological scores in both groups were in strong correlation (r = 0.931 for CCl_4 plus taurine and r = 0.899 for CCl_4 plus saline group). CONCLUSION: Organelle based transmission electron microscopy fi ndings can reflect successfully histological results as well as tissue healing in hepatocytes from hepatotoxin-induced liver fi brosis.
