The article represents the results of the study of the micronutrient deficiency effect(iron and iodine)on the morphometric and optical indices of the oral cavity mucous membrane(OCMM)in school children.In order to ach...The article represents the results of the study of the micronutrient deficiency effect(iron and iodine)on the morphometric and optical indices of the oral cavity mucous membrane(OCMM)in school children.In order to achieve this goal,99 school children aged 6-18 years with adequate iodine and iron supply(control group),latent iron deficiency,and mild iodine deficiency were examined.The morphodensitometric study included the determination of the perimeter and area of cells and nuclei,the nuclear-cytoplasmic ratio,the state of chromatin condensation of the epitheliocyte OCMM.The study material was:epitheliocytes of the buccal scraping.It was determined that the area of epitheliocytes in girls aged 6-11 has significantly increased in mild iodine deficiency and in latent iron deficiency.Instead,the decrease of nuclei area was more noticeable in boys of this age in mild iodine deficiency.During the analysis of the general level of chromatin condensation of epitheliocyte nuclei in children,a gender peculiarity has been proved,which was manifested by a greater sensitivity of girls to microelementosis(increase of the integrative optical density of nuclei in the latent iron deficiency at 97.9%,p1-3<0.05,and cell area at 45.8%,p1-4<0.01 relative to control).Among children aged 12-18 years,young men were more sensitive to the deficiency of the studied trace elements.Significant growth of the cell area at 38.7%(p1-2<0.05)in adolescents with mild iodine deficiency and total optical density of nuclei at 63.6%(p1-2<0.05)was substantiated with respect to control in the decrease of karyoplasmic area.展开更多
The research was carried on 60 rats,which were divided into two experimental groups animals with thyroid hypofunction against iodine deficiency and on the background of combined iodine and copper deficit.The control g...The research was carried on 60 rats,which were divided into two experimental groups animals with thyroid hypofunction against iodine deficiency and on the background of combined iodine and copper deficit.The control group included 30 intact rats.For evaluation of copper balance its content was determined in erythrocyte mass and myocardium.Prooxidant-antioxidant system was examined by the level of peroxide oxidation of proteins and lipids and due to the activity of antioxidant enzymes.Structural features of the myocardium were examined by light-optical microscopy with subsequent morphometry.In the result of the experiment in rats with thyroid hypofunction on the background of combined iodine and copper deficit the redistribution of copper content in the studied tissues was established(accumulation in blood).The results of the study confirm the activation of oxygen-dependent processes in the myocardium of experimental animals,primarily due to peroxide oxidation of proteins,which is especially manifested in the context of combined iodine and copper deficiency.Metabolic changes in the myocardium were confirmed by histology.So,the development of thyroid hypofunction on the background of combined iodine and copper deficiency increases the probability of cardiovascular pathology due to disorders of prooxidant-antioxidant balance and structural changes in the myocardium.展开更多
文摘The article represents the results of the study of the micronutrient deficiency effect(iron and iodine)on the morphometric and optical indices of the oral cavity mucous membrane(OCMM)in school children.In order to achieve this goal,99 school children aged 6-18 years with adequate iodine and iron supply(control group),latent iron deficiency,and mild iodine deficiency were examined.The morphodensitometric study included the determination of the perimeter and area of cells and nuclei,the nuclear-cytoplasmic ratio,the state of chromatin condensation of the epitheliocyte OCMM.The study material was:epitheliocytes of the buccal scraping.It was determined that the area of epitheliocytes in girls aged 6-11 has significantly increased in mild iodine deficiency and in latent iron deficiency.Instead,the decrease of nuclei area was more noticeable in boys of this age in mild iodine deficiency.During the analysis of the general level of chromatin condensation of epitheliocyte nuclei in children,a gender peculiarity has been proved,which was manifested by a greater sensitivity of girls to microelementosis(increase of the integrative optical density of nuclei in the latent iron deficiency at 97.9%,p1-3<0.05,and cell area at 45.8%,p1-4<0.01 relative to control).Among children aged 12-18 years,young men were more sensitive to the deficiency of the studied trace elements.Significant growth of the cell area at 38.7%(p1-2<0.05)in adolescents with mild iodine deficiency and total optical density of nuclei at 63.6%(p1-2<0.05)was substantiated with respect to control in the decrease of karyoplasmic area.
文摘The research was carried on 60 rats,which were divided into two experimental groups animals with thyroid hypofunction against iodine deficiency and on the background of combined iodine and copper deficit.The control group included 30 intact rats.For evaluation of copper balance its content was determined in erythrocyte mass and myocardium.Prooxidant-antioxidant system was examined by the level of peroxide oxidation of proteins and lipids and due to the activity of antioxidant enzymes.Structural features of the myocardium were examined by light-optical microscopy with subsequent morphometry.In the result of the experiment in rats with thyroid hypofunction on the background of combined iodine and copper deficit the redistribution of copper content in the studied tissues was established(accumulation in blood).The results of the study confirm the activation of oxygen-dependent processes in the myocardium of experimental animals,primarily due to peroxide oxidation of proteins,which is especially manifested in the context of combined iodine and copper deficiency.Metabolic changes in the myocardium were confirmed by histology.So,the development of thyroid hypofunction on the background of combined iodine and copper deficiency increases the probability of cardiovascular pathology due to disorders of prooxidant-antioxidant balance and structural changes in the myocardium.
