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应用活体冷冻技术动态观察各种血流动力学改变时血清白蛋白在小鼠近端肾小管的分布变化
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作者 李子龙 N.Terada +1 位作者 n.ohno S.Ohno 《上海医学》 CAS CSCD 北大核心 2009年第6期520-523,I0002,共5页
目的应用活体冷冻技术结合免疫组织化学的方法形象化表现各种血流动力学条件下小鼠血清白蛋白经肾小球毛细血管袢滤过后,在近曲小管再吸收的病理生理过程。方法分别在正常血流、急性高血压和心脏骤停等血流动力学条件下,对模型小鼠的左... 目的应用活体冷冻技术结合免疫组织化学的方法形象化表现各种血流动力学条件下小鼠血清白蛋白经肾小球毛细血管袢滤过后,在近曲小管再吸收的病理生理过程。方法分别在正常血流、急性高血压和心脏骤停等血流动力学条件下,对模型小鼠的左肾进行活体冷冻及冷冻置换。连续的石蜡切片后,分别进行苏木精-伊红染色和抗小鼠白蛋白抗体免疫组织化学染色。结果正常血压和心脏骤停情况下,血清白蛋白在血管内清晰定位,少量出现在近端小管顶端的细胞质部分,但在急性高血压情况下,血清白蛋白明显地免疫定位于所有近端小管。结论活体冷冻技术结合冷冻置换方法可以原位捕获瞬间被活体功能状态的近端肾小管上皮细胞再吸收的血清白蛋白,可视化活体组织细胞的功能形态学特征。 展开更多
关键词 活体冷冻技术 急性高血压 小鼠肾脏 免疫组织化学 血清白蛋白
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Fatigue properties of rolled AZ31B magnesium alloy plate 被引量:3
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作者 S.MORITA n.ohno +1 位作者 F.TAMAI Y.KAWAKAMI 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2010年第B07期523-526,共4页
Fatigue strength,crack initiation and propagation behavior of rolled AZ31B magnesium alloy plate were investigated. Axial tension-compression fatigue tests were carried out with cylindrical smooth specimens.Two types ... Fatigue strength,crack initiation and propagation behavior of rolled AZ31B magnesium alloy plate were investigated. Axial tension-compression fatigue tests were carried out with cylindrical smooth specimens.Two types of specimens were machined with the loading axis parallel(L-specimen)and perpendicular(T-specimen)to rolling direction.Monotonic compressive 0.2%proof stress,tensile strength and tensile elongation were similar for both specimens.On the other hand,monotonic tensile 0.2%proof stress of the L-specimen was slightly higher than that of the T-specimen.Moreover,monotonic compressive 0.2%proof stresses were lower than tensile ones for both specimens.The fatigue strengths of 107cycles of the L-and T-specimens were 95 and 85 MPa,respectively. Compared with the monotonic compressive 0.2%proof stresses,the fatigue strengths were higher for both specimens.In other words, the fatigue crack did not initiate and propagate even though deformation twins were formed in compressive stress under the cyclic tension-compression loading.The fatigue crack initiated at early stage of the fatigue life in low cycle regime regardless of specimen direction.The crack growth rate of the L-specimen was slightly lower than of the T-specimen.Consequently,the fatigue lives of the L-specimen were longer than those of the T-specimen in low cycle regime. 展开更多
关键词 AZ31B镁合金 疲劳性能 低循环疲劳寿命 拉伸试样 轧板 疲劳强度 轴向拉压 拉伸强度
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