During the operational process of natural gas gathering and transmission pipelines,the formation of hydrates is highly probable,leading to uncontrolled movement and aggregation of hydrates.The continuous migration and...During the operational process of natural gas gathering and transmission pipelines,the formation of hydrates is highly probable,leading to uncontrolled movement and aggregation of hydrates.The continuous migration and accumulation of hydrates further contribute to the obstruction of natural gas pipelines,resulting in production reduction,shutdowns,and pressure build-ups.Consequently,a cascade of risks is prone to occur.To address this issue,this study focuses on the operational process of natural gas gathering and transmission pipelines,where a comprehensive framework is established.This framework includes theoretical models for pipeline temperature distribution,pipeline pressure distribution,multiphase flow within the pipeline,hydrate blockage,and numerical solution methods.By analyzing the influence of inlet temperature,inlet pressure,and terminal pressure on hydrate formation within the pipeline,the sensitivity patterns of hydrate blockage risks are derived.The research indicates that reducing inlet pressure and terminal pressure could lead to a decreased maximum hydrate formation rate,potentially mitigating pipeline blockage during natural gas transportation.Furthermore,an increase in inlet temperature and terminal pressure,and a decrease in inlet pressure,results in a displacement of the most probable location for hydrate blockage towards the terminal station.However,it is crucial to note that operating under low-pressure conditions significantly elevates energy consumption within the gathering system,contradicting the operational goal of energy efficiency and reduction of energy consumption.Consequently,for high-pressure gathering pipelines,measures such as raising the inlet temperature or employing inhibitors,electrical heat tracing,and thermal insulation should be adopted to prevent hydrate formation during natural gas transportation.Moreover,considering abnormal conditions such as gas well production and pipeline network shutdowns,which could potentially trigger hydrate formation,the installation of methanol injection connectors remains necessary to ensure production safety.展开更多
A novel approach for analyzing coupled vibrations between vehicles and bridges is presented,taking into account spatiotemporal effects and mechanical phenomena resulting fromvehicle braking.Efficient modeling and solu...A novel approach for analyzing coupled vibrations between vehicles and bridges is presented,taking into account spatiotemporal effects and mechanical phenomena resulting fromvehicle braking.Efficient modeling and solution of bridge vibrations induced by vehicle deceleration are realized using this method.The method’s validity and reliability are substantiated through numerical examples.A simply supported beam bridge with a corrugated steel web is taken as an example and the effects of parameters such as the initial vehicle speed,braking acceleration,braking location,and road surface roughness on the mid-span displacement and impact factor of the bridge are analyzed.The results show that vehicle braking significantly amplifies mid-span displacement and impact factor responses in comparison to uniform vehicular motion across the bridge.Notably,the influence of wheelto-bridge friction forces is of particular significance and cannot be overlooked.When the vehicle initiates braking near the middle of the span,both the mid-span displacement and impact factor of the bridge exhibit substantial increases,further escalating with higher braking acceleration.Under favorable road surface conditions,the midspan displacement and the impact factor during vehicle braking may exceed the design values stipulated by codes.It is important to note that road surface roughness exerts a more pronounced effect on the impact factor of the bridge in comparison to the effects of vehicle braking.展开更多
OBJECTIVES To investigate the prevalence of polypharmacy and potentially inappropriate medication(PIM)in elderly patients with heart failure(HF)and their impact on readmission and mortality.METHODS We conducted a stud...OBJECTIVES To investigate the prevalence of polypharmacy and potentially inappropriate medication(PIM)in elderly patients with heart failure(HF)and their impact on readmission and mortality.METHODS We conducted a study of 274 participants aged 60 years or older with HF.The prevalence of polypharmacy(defined as the use of five or more medications)was calculated,and the 2019 American Geriatrics Society Beers criteria were applied to access PIMs.Medications and PIMs were characterized at admission and discharge,and changes in prescriptions during hospitalization were compared.The impact of polypharmacy and PIM on readmission and mortality were investigated.RESULTS The median age of this study population was 68 years old.The median number of prescribed drugs was 7 at admission and 10 at discharge.At discharge,99.27%of all patients were taking five or more drugs.The incidence of composite endpoint and cardiovascular readmission increased with the number of polypharmacy within 6 months.The use of guideline-directed medical therapy reduced the incidence of composite endpoint events and cardiovascular readmission,while the use of noncardiovascular medications increased the composite endpoint events.The frequency of PIMs was 93.79%at discharge.The incidence of composite endpoint events increased with the number of PIMs.“PIMs in older adults with caution”increased cardiovascular readmission and“PIMs based on kidney function”increased cardiovascular mortality.Several comorbidities were associated with cardiovascular mortality or non-cardiovascular readmission.CONCLUSIONS Polypharmacy and PIM were highly prevalent in elderly patients with HF,and their use was associated with an increased risk of composite endpoint events,readmission and mortality.Non-cardiovascular medications,“PIMs in older adults with caution”,“PIMs based on kidney function”and several comorbidities were important factors associated with hospital readmission and mortality.Our findings highlight the importance of medication optimization in the management of HF in elderly patients.展开更多
As one of the Analysis Centers(AC)of the International GNSS Service(IGS),Wuhan University(WHU)has been contributing to the IGS by providing ultra-rapid as well as rapid orbit and clock solutions for the established GP...As one of the Analysis Centers(AC)of the International GNSS Service(IGS),Wuhan University(WHU)has been contributing to the IGS by providing ultra-rapid as well as rapid orbit and clock solutions for the established GPS and GLONASS since 2012.In the same year,the IGS initiated the Multi-GNSS Experiment(MGEX)to support the analysis of the emerging GNSS systems and prepare the IGS for Multi-GNSS,which includes GPS,GLONASS,the European Galileo system,the Chinese Beidou Navigation Satellite System(BDS),the Japanese Quasi-Zenith Satellite System(QZSS)and the Indian Regional Navigation Satellite System(IRNSS/NaVIC).The major products,i.e.,orbits,Earth Orientation Parameters(EOPs),satellite clock as well as attitude have also been provided by WHU since 2012.More recently,WHU has engaged the third reprocessing of IGS for generating the highly accurate station coordinates as inputs for establishment of the International Terrestrial Reference Frame(ITRF)2020 during 2019—2020.This article presents the recent major advancements of the IGS AC at Wuhan University,including precise products,real-time products,bias products,antenna phase center calibration,and the non-linear motion modeling for GNSS Reference Stations.展开更多
Objective:To study the effect of pyrrolidine dithiocarbamate(PDTC) on the anti-tuberculosis drug-induced liver injury and the molecular mechanism. Methods:Clean male SD rats were selected as experimental animals and r...Objective:To study the effect of pyrrolidine dithiocarbamate(PDTC) on the anti-tuberculosis drug-induced liver injury and the molecular mechanism. Methods:Clean male SD rats were selected as experimental animals and randomly divided into normal group,model group,PDTC group and AG490 group. Animal model of anti-tuberculosis drug-induced liver injury was established by intragastric administration isoniazid + rifampicin. PDTC group received intraperitoneal injection of PDTC,and AG490 group received intraperitoneal injection of AG490. Twenty-eight days after intervention,the rats were executed,and the liver injury indexes,inflammation indexes and oxidative stress indexes in serum as well as JAK2/STAT3 expression,liver injury indexes,inflammation indexes and oxidative stress indexes in liver tissue were determined. Results:p-JAK2,p-STAT3,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA expression in liver tissue as well as TBIL,ALT,AST,γ-GT,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA levels in serum of model group were significantly higher than those of normal group while p-JAK2,p-STAT3,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA expression in liver tissu as well as TBIL,ALT,AST,γ-GT,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA levels in serum of PDTC group and AG490 group were significantly lower than those of model group. Conclusions:PDTC can inhibit the inflammation and oxidative stress mediated by JAK2/STAT3 signaling pathway to alleviate the anti-tuberculosis drug-induced liver injury.展开更多
A 72-h high-resolution simulation of Supertyphoon Rammasun （2014） is performed using the Advanced Research Weather Research and Forecasting model. The model covers an initial 18-h spin-up, the 36-h rapid intensifica...A 72-h high-resolution simulation of Supertyphoon Rammasun （2014） is performed using the Advanced Research Weather Research and Forecasting model. The model covers an initial 18-h spin-up, the 36-h rapid intensification （RI） period in the northern South China Sea, and the 18-h period of weakening after landfall. The results show that the model reproduces the track, intensity, structure of the storm, and environmental circulations reasonably well. Analysis of the surface energetics under the storm indicates that the storm＇s intensification is closely related to the net energy gain rate （eg）, defined as the difference between the energy production （PD） due to surface entropy flux and the energy dissipation （Ds） due to surface friction near the radius of maximum wind （RMW）. Before and during the RI stage, the ~：g is high, indicating sufficient energy supply for the storm to intensify. However, the Sg decreases rapidly as the storm quickly intensifies, because the Ds increases more rapidly than the PD near the RMW. By the time the storm reaches its peak intensity, the Ds is about 20% larger than the PD near the RMW, leading to a local energetics deficit under the eyewall. During the mature stage, the PD and Ds can reach a balance within a radius of 86 km from the storm center （about 2.3 times the RMW）. This implies that the local PD under the eyewall is not large enough to balance the Ds, and the radially inward energy transport from outside the eyewall must play an important role in maintaining the storm＇s intensity, as well as its intensification.展开更多
Red tides are a major public hazard in the global oceans.The coast of the East China Sea is the sea area where red tide disasters are the most frequent and serious in China.In order to accurately grasp the occurrence ...Red tides are a major public hazard in the global oceans.The coast of the East China Sea is the sea area where red tide disasters are the most frequent and serious in China.In order to accurately grasp the occurrence of red tides in the coastal waters of the East China Sea,and to understand the microbial communities in the waters during the occurrence of red tides in the East China Sea,a special survey of red tides in the coastal waters of Zhejiang,China was carried out in June 2018.The results showed that nutrient concentrations of N and P were generally high in this area,DIN concentrations in most areas exceeded the permitted limit of Chinese seawater quality grade I.There were significant differences in dissolved oxygen,pH,COD,chlorophyll and phytoplankton abundance of red tides.During the investigation,red tides were found in the waters near the Yushan Islands.The content of chlorophyll a was 42.12mg/m3,the cell abundance of phytoplankton was 8.16×108/L,and the abundance of Prorocentrum edulis accounted for 98.5%.The Illumina MiSeq sequencing platform was used for 16s high-throughput sequencing of water microorganisms,and a total of 16 bacteria were identified.Proteobacteria is the first dominant phylum,followed by Cyanobacteria and Bacteroides.Some differences in bacterial community compositions between HAB and the nearby seawater were observed.The predominant bacteria in the red tide occurrence area were Proteobacteria,comprising 46.1%of the relative abundance;while the predominant bacteria in the nearby sea area,comprising 42.0%of the relative abundance.展开更多
Transient receptor potential vanillic acid receptor 1(TRPV1)is a member of the(TRP)ion channel family of transient receptor potentials.Studies have shown that TRPV1 plays an important regulatory role in gastrointestin...Transient receptor potential vanillic acid receptor 1(TRPV1)is a member of the(TRP)ion channel family of transient receptor potentials.Studies have shown that TRPV1 plays an important regulatory role in gastrointestinal diseases,especially in inflammatory pain,visceral pain and cancer pain-mediated diseases.In recent years,the active components of traditional Chinese medicine have been widely studied as drugs for the treatment of gastrointestinal diseases,in which there is no lack of traditional Chinese medicine to explore the regulation of TRPV1 channels in order to be used as a new therapeutic target in the treatment of gastrointestinal diseases.This article reviews the research progress on the relationship between traditional Chinese medicine and gastrointestinal diseases which regulate TRPV1 signal pathway as a therapeutic target.展开更多
Objective:To make Galangin-PLGA nanoparticles(GL-PLGA NPs)were prepared using the biodegradable material polylactic-coglycolic acid(PLGA)as the carrier material,and the formulation was optimized.Methods:Nanoparticles ...Objective:To make Galangin-PLGA nanoparticles(GL-PLGA NPs)were prepared using the biodegradable material polylactic-coglycolic acid(PLGA)as the carrier material,and the formulation was optimized.Methods:Nanoparticles were prepared by modified emulsification-solvent evaporation method and the content of galangin was determined by HPLC.With particle size and entrapment efficiency as the indexes,single factor and orthogonal test were used to optimize the formulation,and the quality of the optimized GL-PLGA NPs was evaluated from the surface configuration,particle size distribution,Zeta potential and entrapment efficiency.Results:The optimal prescription conditions of GL-PLGA NPs were:the volume ratio of organic phase to aqueous phase was 1:8,the concentration of PVA was 1.5%,the concentration of PLGA was 1.0%,and the drug concentration was 0.2%.Under these conditions,the average particle size of GL-PLGA NPs was 249±1.32nm,polydispersity index(PDI)was 0.059,Zeta potential was-4.86mV,and the entrapment efficiency was 75.3%.The results of electron microscopy showed that the nanoparticles were spherical,uniform particle size and good dispersion.Conclusion:The preparation method is simple and stable,and GL-PLGA NPs with suitable particle size and high entrapment efficiency can be obtained.展开更多
基金supported by 111 Project (No.D21025)Open Fund Project of State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation (Nos.PLN2021-01,PLN2021-02,PLN2021-03)+2 种基金High-end Foreign Expert Introduction Program (No.G2021036005L)National Key Research and Development Program (No.2021YFC2800903)National Natural Science Foundation of China (No.U20B6005-05)。
文摘During the operational process of natural gas gathering and transmission pipelines,the formation of hydrates is highly probable,leading to uncontrolled movement and aggregation of hydrates.The continuous migration and accumulation of hydrates further contribute to the obstruction of natural gas pipelines,resulting in production reduction,shutdowns,and pressure build-ups.Consequently,a cascade of risks is prone to occur.To address this issue,this study focuses on the operational process of natural gas gathering and transmission pipelines,where a comprehensive framework is established.This framework includes theoretical models for pipeline temperature distribution,pipeline pressure distribution,multiphase flow within the pipeline,hydrate blockage,and numerical solution methods.By analyzing the influence of inlet temperature,inlet pressure,and terminal pressure on hydrate formation within the pipeline,the sensitivity patterns of hydrate blockage risks are derived.The research indicates that reducing inlet pressure and terminal pressure could lead to a decreased maximum hydrate formation rate,potentially mitigating pipeline blockage during natural gas transportation.Furthermore,an increase in inlet temperature and terminal pressure,and a decrease in inlet pressure,results in a displacement of the most probable location for hydrate blockage towards the terminal station.However,it is crucial to note that operating under low-pressure conditions significantly elevates energy consumption within the gathering system,contradicting the operational goal of energy efficiency and reduction of energy consumption.Consequently,for high-pressure gathering pipelines,measures such as raising the inlet temperature or employing inhibitors,electrical heat tracing,and thermal insulation should be adopted to prevent hydrate formation during natural gas transportation.Moreover,considering abnormal conditions such as gas well production and pipeline network shutdowns,which could potentially trigger hydrate formation,the installation of methanol injection connectors remains necessary to ensure production safety.
基金supported by the Henan Provincial Science and Technology Research Project under Grant(152102310295).
文摘A novel approach for analyzing coupled vibrations between vehicles and bridges is presented,taking into account spatiotemporal effects and mechanical phenomena resulting fromvehicle braking.Efficient modeling and solution of bridge vibrations induced by vehicle deceleration are realized using this method.The method’s validity and reliability are substantiated through numerical examples.A simply supported beam bridge with a corrugated steel web is taken as an example and the effects of parameters such as the initial vehicle speed,braking acceleration,braking location,and road surface roughness on the mid-span displacement and impact factor of the bridge are analyzed.The results show that vehicle braking significantly amplifies mid-span displacement and impact factor responses in comparison to uniform vehicular motion across the bridge.Notably,the influence of wheelto-bridge friction forces is of particular significance and cannot be overlooked.When the vehicle initiates braking near the middle of the span,both the mid-span displacement and impact factor of the bridge exhibit substantial increases,further escalating with higher braking acceleration.Under favorable road surface conditions,the midspan displacement and the impact factor during vehicle braking may exceed the design values stipulated by codes.It is important to note that road surface roughness exerts a more pronounced effect on the impact factor of the bridge in comparison to the effects of vehicle braking.
基金The authors express their gratitude to all the hospital staff who assisted with data retrieval from the electronic medical record database and routine telephone follow-up.Additionally,the authors would like to extend their appreciation to Wenhui Liu for providing statistical guidance at the School of Public Health,Shandong University.The authors declare that they have no conflicts of interests to disclose.
文摘OBJECTIVES To investigate the prevalence of polypharmacy and potentially inappropriate medication(PIM)in elderly patients with heart failure(HF)and their impact on readmission and mortality.METHODS We conducted a study of 274 participants aged 60 years or older with HF.The prevalence of polypharmacy(defined as the use of five or more medications)was calculated,and the 2019 American Geriatrics Society Beers criteria were applied to access PIMs.Medications and PIMs were characterized at admission and discharge,and changes in prescriptions during hospitalization were compared.The impact of polypharmacy and PIM on readmission and mortality were investigated.RESULTS The median age of this study population was 68 years old.The median number of prescribed drugs was 7 at admission and 10 at discharge.At discharge,99.27%of all patients were taking five or more drugs.The incidence of composite endpoint and cardiovascular readmission increased with the number of polypharmacy within 6 months.The use of guideline-directed medical therapy reduced the incidence of composite endpoint events and cardiovascular readmission,while the use of noncardiovascular medications increased the composite endpoint events.The frequency of PIMs was 93.79%at discharge.The incidence of composite endpoint events increased with the number of PIMs.“PIMs in older adults with caution”increased cardiovascular readmission and“PIMs based on kidney function”increased cardiovascular mortality.Several comorbidities were associated with cardiovascular mortality or non-cardiovascular readmission.CONCLUSIONS Polypharmacy and PIM were highly prevalent in elderly patients with HF,and their use was associated with an increased risk of composite endpoint events,readmission and mortality.Non-cardiovascular medications,“PIMs in older adults with caution”,“PIMs based on kidney function”and several comorbidities were important factors associated with hospital readmission and mortality.Our findings highlight the importance of medication optimization in the management of HF in elderly patients.
基金National Natural Science Foundation of China(Nos.42030109,42074032)Program for Hubei Provincial Science and Technology Innovation Talants(No.2022EJD010)。
文摘As one of the Analysis Centers(AC)of the International GNSS Service(IGS),Wuhan University(WHU)has been contributing to the IGS by providing ultra-rapid as well as rapid orbit and clock solutions for the established GPS and GLONASS since 2012.In the same year,the IGS initiated the Multi-GNSS Experiment(MGEX)to support the analysis of the emerging GNSS systems and prepare the IGS for Multi-GNSS,which includes GPS,GLONASS,the European Galileo system,the Chinese Beidou Navigation Satellite System(BDS),the Japanese Quasi-Zenith Satellite System(QZSS)and the Indian Regional Navigation Satellite System(IRNSS/NaVIC).The major products,i.e.,orbits,Earth Orientation Parameters(EOPs),satellite clock as well as attitude have also been provided by WHU since 2012.More recently,WHU has engaged the third reprocessing of IGS for generating the highly accurate station coordinates as inputs for establishment of the International Terrestrial Reference Frame(ITRF)2020 during 2019—2020.This article presents the recent major advancements of the IGS AC at Wuhan University,including precise products,real-time products,bias products,antenna phase center calibration,and the non-linear motion modeling for GNSS Reference Stations.
基金supported by Surface Project of Shandong Provincial Natural Science Foundation(No.ZR2014HM081)
文摘Objective:To study the effect of pyrrolidine dithiocarbamate(PDTC) on the anti-tuberculosis drug-induced liver injury and the molecular mechanism. Methods:Clean male SD rats were selected as experimental animals and randomly divided into normal group,model group,PDTC group and AG490 group. Animal model of anti-tuberculosis drug-induced liver injury was established by intragastric administration isoniazid + rifampicin. PDTC group received intraperitoneal injection of PDTC,and AG490 group received intraperitoneal injection of AG490. Twenty-eight days after intervention,the rats were executed,and the liver injury indexes,inflammation indexes and oxidative stress indexes in serum as well as JAK2/STAT3 expression,liver injury indexes,inflammation indexes and oxidative stress indexes in liver tissue were determined. Results:p-JAK2,p-STAT3,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA expression in liver tissue as well as TBIL,ALT,AST,γ-GT,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA levels in serum of model group were significantly higher than those of normal group while p-JAK2,p-STAT3,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA expression in liver tissu as well as TBIL,ALT,AST,γ-GT,TNF-α,IL-1β,IL-6,ROS,8-OHdG and MDA levels in serum of PDTC group and AG490 group were significantly lower than those of model group. Conclusions:PDTC can inhibit the inflammation and oxidative stress mediated by JAK2/STAT3 signaling pathway to alleviate the anti-tuberculosis drug-induced liver injury.
基金supported by the National Basic Research and Development Project (973 program) of China (Grant No. 2015CB452805)the National Natural Science Foundation of China (Grant No. 41375068)partly supported by the National Science Foundation (Grant No. AGS-1326524)
文摘A 72-h high-resolution simulation of Supertyphoon Rammasun （2014） is performed using the Advanced Research Weather Research and Forecasting model. The model covers an initial 18-h spin-up, the 36-h rapid intensification （RI） period in the northern South China Sea, and the 18-h period of weakening after landfall. The results show that the model reproduces the track, intensity, structure of the storm, and environmental circulations reasonably well. Analysis of the surface energetics under the storm indicates that the storm＇s intensification is closely related to the net energy gain rate （eg）, defined as the difference between the energy production （PD） due to surface entropy flux and the energy dissipation （Ds） due to surface friction near the radius of maximum wind （RMW）. Before and during the RI stage, the ~：g is high, indicating sufficient energy supply for the storm to intensify. However, the Sg decreases rapidly as the storm quickly intensifies, because the Ds increases more rapidly than the PD near the RMW. By the time the storm reaches its peak intensity, the Ds is about 20% larger than the PD near the RMW, leading to a local energetics deficit under the eyewall. During the mature stage, the PD and Ds can reach a balance within a radius of 86 km from the storm center （about 2.3 times the RMW）. This implies that the local PD under the eyewall is not large enough to balance the Ds, and the radially inward energy transport from outside the eyewall must play an important role in maintaining the storm＇s intensity, as well as its intensification.
基金National Key Research and Development Program“High-resolution Numerical Simulation and Prediction of Ecological Environment in the Yellow and East China Seas”(Project No.:2016YFC1401603)Scientific Research Project of Department of Ecology and Environment of Zhejiang Province(Project No.:2016A012).
文摘Red tides are a major public hazard in the global oceans.The coast of the East China Sea is the sea area where red tide disasters are the most frequent and serious in China.In order to accurately grasp the occurrence of red tides in the coastal waters of the East China Sea,and to understand the microbial communities in the waters during the occurrence of red tides in the East China Sea,a special survey of red tides in the coastal waters of Zhejiang,China was carried out in June 2018.The results showed that nutrient concentrations of N and P were generally high in this area,DIN concentrations in most areas exceeded the permitted limit of Chinese seawater quality grade I.There were significant differences in dissolved oxygen,pH,COD,chlorophyll and phytoplankton abundance of red tides.During the investigation,red tides were found in the waters near the Yushan Islands.The content of chlorophyll a was 42.12mg/m3,the cell abundance of phytoplankton was 8.16×108/L,and the abundance of Prorocentrum edulis accounted for 98.5%.The Illumina MiSeq sequencing platform was used for 16s high-throughput sequencing of water microorganisms,and a total of 16 bacteria were identified.Proteobacteria is the first dominant phylum,followed by Cyanobacteria and Bacteroides.Some differences in bacterial community compositions between HAB and the nearby seawater were observed.The predominant bacteria in the red tide occurrence area were Proteobacteria,comprising 46.1%of the relative abundance;while the predominant bacteria in the nearby sea area,comprising 42.0%of the relative abundance.
基金National Natural Science Foundation of China(No.81660649)。
文摘Transient receptor potential vanillic acid receptor 1(TRPV1)is a member of the(TRP)ion channel family of transient receptor potentials.Studies have shown that TRPV1 plays an important regulatory role in gastrointestinal diseases,especially in inflammatory pain,visceral pain and cancer pain-mediated diseases.In recent years,the active components of traditional Chinese medicine have been widely studied as drugs for the treatment of gastrointestinal diseases,in which there is no lack of traditional Chinese medicine to explore the regulation of TRPV1 channels in order to be used as a new therapeutic target in the treatment of gastrointestinal diseases.This article reviews the research progress on the relationship between traditional Chinese medicine and gastrointestinal diseases which regulate TRPV1 signal pathway as a therapeutic target.
基金National natural science foundation of China(No.81660649)Innovation and entrepreneurship training program for undergraduates of Hainan medical university in 2019(No.X201911810073)。
文摘Objective:To make Galangin-PLGA nanoparticles(GL-PLGA NPs)were prepared using the biodegradable material polylactic-coglycolic acid(PLGA)as the carrier material,and the formulation was optimized.Methods:Nanoparticles were prepared by modified emulsification-solvent evaporation method and the content of galangin was determined by HPLC.With particle size and entrapment efficiency as the indexes,single factor and orthogonal test were used to optimize the formulation,and the quality of the optimized GL-PLGA NPs was evaluated from the surface configuration,particle size distribution,Zeta potential and entrapment efficiency.Results:The optimal prescription conditions of GL-PLGA NPs were:the volume ratio of organic phase to aqueous phase was 1:8,the concentration of PVA was 1.5%,the concentration of PLGA was 1.0%,and the drug concentration was 0.2%.Under these conditions,the average particle size of GL-PLGA NPs was 249±1.32nm,polydispersity index(PDI)was 0.059,Zeta potential was-4.86mV,and the entrapment efficiency was 75.3%.The results of electron microscopy showed that the nanoparticles were spherical,uniform particle size and good dispersion.Conclusion:The preparation method is simple and stable,and GL-PLGA NPs with suitable particle size and high entrapment efficiency can be obtained.
