Network pharmacology and molecular docking identify mechanisms of medicinal plant-derived 1,2,3,4,6-penta-O-galloyl-beta-D-glucose treating gastric cancer

Background:1,2,3,4,6-penta-O-galloyl-beta-D-glucose(PGG)is a natural polyphenolic compound derived from multiple medicinal plants with favorable anticancer activity.Methods:In this study,the mechanisms of PGG against gastric cancer were explored through network pharmacology and molecular docking.First,the targets of PGG were searched in the Herbal Ingredients’Targets(HIT),Similarity Ensemble Approach(SEA),and Super-PRED databases.The potential targets related to gastric cancer were predicted from the Human Gene Database(GeneCards)and DisGeNET databases.The intersecting targets of PGG and gastric cancer were obtained by Venn diagram and then subjected to protein-protein interaction analysis to screen hub targets.Functional and pathway enrichment of hub targets were analyzed through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway databases.The differential expression and survival analysis of hub targets in gastric cancer were performed based on The Cancer Genome Atlas database.Finally,the affinity of PGG with hub targets was visualized by molecular docking.Results:Three hub targets were screened,including mitogen-activated protein kinase 14(MAPK14),BCL2 like 1(BCL2L1),and vascular endothelial growth factor A(VEGFA).MAPK14 had a higher expression,while BCL2L1 and VEGFA had lower expression in gastric cancer than in normal conditions.Enrichment analysis indicated enrichment of these hub targets in MAPK,neurotrophin,programmed death-ligand 1(PD-L1)checkpoint,phosphatidylinositol 3-kinases/protein kinase B(PI3K-Akt),Ras,and hypoxia-inducible factor-1(HIF-1)signaling pathways.Conclusion:Therefore,network pharmacology and molecular docking analyses revealed that PGG exerts a therapeutic efficacy on gastric cancer by multiple targets(MAPK14,BCL2L1,and VEGFA)and pathways(MAPK,PD-L1 checkpoint,PI3K-Akt,Ras,and HIF-1 pathways).

肿瘤合并新型冠状病毒感染的临床特征与应对策略

自2019年12月中旬开始,中国湖北省武汉市爆发了新型冠状病毒肺炎疫情,并迅速蔓延至中国其他省市及全球数十个国家和地区,成为国际重大突发公共卫生事件。新型冠状病毒主要通过飞沫和密切接触传播,人群普遍易感。肿瘤患者由于免疫功能受损,更是成为此次疫情中的高危人群。早期识别肿瘤并发新型冠状病毒肺炎患者,了解其分布特征,以便给予更有针对性的治疗,提高患者治愈率,更好地控制新型冠状病毒的流行和发展。本文通过对相关文献的综合分析,对肿瘤合并新型冠状病毒肺炎患者的临床特点进行了较为系统的综述,并为肿瘤患者如何应对此次疫情提出了相关建议。

A Novel Flux-weakening Control Method with Quadrature Voltage Constrain for Electrolytic Capacitorless PMSM Drives

In electrolytic capacitorless permanent magnet synchronous motor(PMSM) drives, the DC-link voltage will fluctuate in a wide range due to the use of slim film capacitor. When the flux-weakening current is lower than-ψf/Ld during the high speed operation, the flux-weakening control loop will transform to a positive feedback mode, which means the reduction of flux-weakening current will lead to the acceleration of the voltage saturation, thus the whole system will be unstable. In order to solve this issue, this paper proposes a novel flux-weakening method for electrolytic capacitorless motor drives to maintain a negative feedback characteristic of the control loop during high speed operation. Based on the analysis of the instability mechanism in flux-weakening region, a quadrature voltage constrain mechanism is constructed to stabilize the system.Meanwhile, the parameters of the controller are theoretically designed for easier industrial application. The proposed algorithm is implemented on a 1.5 kW electrolytic capacitorless PMSM drive to verify the effectiveness of the flux-weakening performance.

Evaluation of Laparoscopy Combined with Intraoperative Gastroscopic Local Gastrectomy in the Treatment of Gastric Neuroendocrine Tumors

Objective:To analyze the clinical effect of laparoscopy combined with intraoperative gastroscopic local gastrectomy in the treatment of gastric neuroendocrine tumors.Methods:A total of 100 patients with gastric neuroendocrine tumors that were treated in the Affiliated Hospital of Chifeng University from January 2016 to March 2021 were selected as the research subjects・They were divided into two groups by the digital table method.The control group underwent laparoscopic partial gastrectomy while the research group underwent laparoscopy combined with intraoperative gastroscopic partial gastrectomy.The curative effects of the two groups were compared.Results:There was no significant difference in the number of surgical lymph node dissections between the two groups(p>0.05);the amount of bleeding in the research group was lower than that in the control group;the operation time,time taken to get out of bed,gastrointestinal fimction recovery time,time taken for first eating,and hospital stay were shorter than those in the control group(p<0.05);the incidence of complications and the recurrence rate in the research group were lower than those in the control group(p<0.05).Conclusion:Laparoscopy combined with intraoperative gastroscopic local gastrectomy in the treatment of gastric neuroendocrine tumors has significant clinical effect and high safety.

Comparative Analysis on the Curative Effect of Endoscopic Submucosal Dissection and Mucosal Resection in the Treatment of Gastric Neuroendocrine Tumor

Objective:To study the therapeutic effect of endoscopic submucosal dissection and mucosal resection on gastric neuroendocrine tumor.Methods:A hundred patients with gastric neuroendocrine tumor that were treated in the Affiliated Hospital of Chifeng University from January 2016 to May 2021 were randomly selected for this research.They were divided into two groups,which were the control group(endoscopic mucosal resection)and the study group(endoscopic submucosal dissection),by the digital table method.The curative effects of the two groups were observed and compared.Results:Before operation,there were no significant differences in serum CgA,TNF-α,and IL-6 between the two groups,p>0.05.After surgical treatment,the operation time and hospital stay of the patients in the study group were shorter than those in the control group,the amount of surgical bleeding was also less compared to the control group,and the complete tumor resection rate was higher than that in the control group(p<0.05);the levels of IL-6 and CgA of the study group were lower than those in the control group,while the levels of TNF-α were higher than those of the control group,p<0.05;the postoperative complication rate of the study group was lower than that of the reference group(p<0.05).Conclusion:Endoscopic submucosal dissection is more effective for gastric neuroendocrine tumors.The resection rate of the tumor is high,and the operation risk is low.

Short telomeres impede germ cell specification by upregulating MAPK and TGFβsignaling

Functional telomeres protect chromosome ends and play important roles in stem cell maintenance and differentiation. Short telomeres negatively impact germ cell development and can contribute to age-associated infertility. Moreover, telomere syndrome resulting from mutations of telomerase or telomere-associated genes exhibits short telomeres and reduced fertility. It remains elusive whether and how telomere lengths affect germ cell specification. We report that functional telomere is required for the coordinated germ cell and somatic cell fate decisions. Using telomerase gene Terc deficient mice as a model, we show that short telomeres restrain germ cell specification of epiblast cells but promote differentiation towards somatic lineage. Short telomeres increase chromatin accessibility to elevate TGFβ and MAPK/ERK signaling for somatic cell differentiation. Notably,elevated Fst expression in TGFβ pathway represses the BMP4-pSmad signaling pathway, thus reducing germ cell formation. Reelongation of telomeres by targeted knock-in of Terc restores normal chromatin accessibility to suppress TGFβ and MAPK signaling, thereby facilitating germ cell formation. Taken together, our data reveal that functional telomeres are required for germ cell specification by repressing TGFβ and MAPK signaling.

Review of Parameter Identification and Sensorless Control Methods for Synchronous Reluctance Machines

Synchronous reluctance machines(SynRMs)have drawn increasing attention in recent years owing to their advantages such as low cost,simple structure,ease of manufacture,and high robustness.The main obstacle to the promotion of SynRMs is severe parameter nonlinearity,which deteriorates drive performance.Sensorless control methods for SynRMs are critical technologies that can broaden the industrial applications of SynRMs.Various methods of parameter identification and sensorless control strategies are reviewed and discussed,including self-commissioning,which is analyzed in detail.Furthermore,sensorless control strategies that can improve the industrial application of SynRMs are described.Finally,future research trends concerning SynRMs are analyzed and discussed.

A Two-color Single-molecule Sequencing Platform and Its Clinical Applications

DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years.In this study,we developed a single-molecule desktop sequencer,GenoCare 1600(GenoCare),which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry,making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use.Using the GenoCare platform,we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%.We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019(COVID-19)samples from throat swabs.Our findings indicate that the GenoCare platform allows for microbial quantitation,sensitive identification of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus,and accurate detection of virus mutations,as confirmed by Sanger sequencing,demonstrating its remarkable potential in clinical application.