The potato rot nematode(Ditylenchus destructor) is a very economically important nematode in agronomic and horticultural plants worldwide. In this study, 43 populations of D. destructor were collected from different h...The potato rot nematode(Ditylenchus destructor) is a very economically important nematode in agronomic and horticultural plants worldwide. In this study, 43 populations of D. destructor were collected from different hosts across China, including 37 populations from Chinese herbal medicine plants. Obtained sequences of ITS-rDNA and D2–D3of 28S-rDNA genes of D. destructor were compared and analyzed. Nine types of significant length variations in ITS sequences were observed among all populations. The differences in ITS1 length were mainly caused by the presence of repetitive elements with substantial base substitutions. Reconstructions of ITS1 secondary structures showed that the minisatellites formed a stem structure. Ten haplotypes were observed in all populations based on mutations and variations of helix H9. Among them, 3 known haplotypes(A–C) were found in 7 populations isolated from potato,sweet potato, and Codonopsis pilosula, and 7 unique haplotypes were found in other 36 populations collected from C. pilosula and Angelica sinensis compared with 7 haplotypes(A–G) according to Subbotin' system. These unique haplotypes were different from haplotypes A–G, and we named them as haplotypes H–N. The present results showed that a total of 14 haplotypes(A–N) of ITS-rDNA have been found in D. destructor. Phylogenetic analyses of ITSrDNA and D2–D3 showed that all populations of D. destructor were clustered into two major clades: one clade only containing haplotype A from sweet potato and the other containing haplotypes B–N from other plants. For further verification, PCR-ITS-RFLP profiles were conducted on 7 new haplotypes. Collectively, our study suggests that D. destructor populations on Chinese medicinal materials are very different from those on other hosts and this work provides a paradigm for relevant researches.展开更多
Background There are few studies on the arterial compliance of noise exposure. The purpose of this study was to understand the relationship between hearing loss, blood pressure and arterial compliance of female worker...Background There are few studies on the arterial compliance of noise exposure. The purpose of this study was to understand the relationship between hearing loss, blood pressure and arterial compliance of female workers who exposed to occupational noise in a textile mill. Methods The noise levels in the workplace were measured with a HS6288 sound level meter. Cumulated noise exposure （CNE） was calculated according to the noise intensity and the exposure period. Hearing ability and arterial compliance were measured in 618 noise exposed workers. The database was set up with EpiData and the statistical analysis was performed with SAS software 9.1.3. Results The noise levels were 80.1dB（A） to 113.5dB（A）, of which the levels at 92.5% of the noise monitoring sites were over the national standard. The incidence of high frequency hearing loss （HFHL） was 24.43% and language frequency hearing impairment （LFHI） was 0.81%. The incidence of hypertension was 7.93%. Both systolic blood pressure （SBP） and diastolic blood pressure （DBP） in the high frequency hearing loss group were significantly higher than those in the normal hearing group （P〈0.05）, while C1 （large artery compliance） and C2 （small artery compliance） were significantly lower （P〈0.05）. The high frequency hearing threshold （HFHT） of the hypertension group was significantly higher than in the normal blood pressure group （P〈0.05）, while C1 and C2 were significantly lower （P〈0.05）.C1 and C2 had a negative correlation with HFHT, SBP, DBP, mean of arterial pressure （MAP）, pulse pressure （PP） and pulse rate （PR） （P〈0.05）. The multiple regression analyses showed that blood pressure and PR were the main influencing factors on C1 and C2. LFHT was an influence on C2 and HFHT on C1. Conclusions Textile mill noise pollution is very serious and has an obvious influence on worker＇s auditory function. The female workers with low artery compliance or with high blood pressure might be suffering from hearing loss; those with noise induced hearing loss might be suffering from hypertension if she is constantly exposed to loud industrial noise.展开更多
基金supported by the National Natural Science Foundation of China (31760507)the National Key R&D Program of China (2018YFC1706301)。
文摘The potato rot nematode(Ditylenchus destructor) is a very economically important nematode in agronomic and horticultural plants worldwide. In this study, 43 populations of D. destructor were collected from different hosts across China, including 37 populations from Chinese herbal medicine plants. Obtained sequences of ITS-rDNA and D2–D3of 28S-rDNA genes of D. destructor were compared and analyzed. Nine types of significant length variations in ITS sequences were observed among all populations. The differences in ITS1 length were mainly caused by the presence of repetitive elements with substantial base substitutions. Reconstructions of ITS1 secondary structures showed that the minisatellites formed a stem structure. Ten haplotypes were observed in all populations based on mutations and variations of helix H9. Among them, 3 known haplotypes(A–C) were found in 7 populations isolated from potato,sweet potato, and Codonopsis pilosula, and 7 unique haplotypes were found in other 36 populations collected from C. pilosula and Angelica sinensis compared with 7 haplotypes(A–G) according to Subbotin' system. These unique haplotypes were different from haplotypes A–G, and we named them as haplotypes H–N. The present results showed that a total of 14 haplotypes(A–N) of ITS-rDNA have been found in D. destructor. Phylogenetic analyses of ITSrDNA and D2–D3 showed that all populations of D. destructor were clustered into two major clades: one clade only containing haplotype A from sweet potato and the other containing haplotypes B–N from other plants. For further verification, PCR-ITS-RFLP profiles were conducted on 7 new haplotypes. Collectively, our study suggests that D. destructor populations on Chinese medicinal materials are very different from those on other hosts and this work provides a paradigm for relevant researches.
文摘Background There are few studies on the arterial compliance of noise exposure. The purpose of this study was to understand the relationship between hearing loss, blood pressure and arterial compliance of female workers who exposed to occupational noise in a textile mill. Methods The noise levels in the workplace were measured with a HS6288 sound level meter. Cumulated noise exposure （CNE） was calculated according to the noise intensity and the exposure period. Hearing ability and arterial compliance were measured in 618 noise exposed workers. The database was set up with EpiData and the statistical analysis was performed with SAS software 9.1.3. Results The noise levels were 80.1dB（A） to 113.5dB（A）, of which the levels at 92.5% of the noise monitoring sites were over the national standard. The incidence of high frequency hearing loss （HFHL） was 24.43% and language frequency hearing impairment （LFHI） was 0.81%. The incidence of hypertension was 7.93%. Both systolic blood pressure （SBP） and diastolic blood pressure （DBP） in the high frequency hearing loss group were significantly higher than those in the normal hearing group （P〈0.05）, while C1 （large artery compliance） and C2 （small artery compliance） were significantly lower （P〈0.05）. The high frequency hearing threshold （HFHT） of the hypertension group was significantly higher than in the normal blood pressure group （P〈0.05）, while C1 and C2 were significantly lower （P〈0.05）.C1 and C2 had a negative correlation with HFHT, SBP, DBP, mean of arterial pressure （MAP）, pulse pressure （PP） and pulse rate （PR） （P〈0.05）. The multiple regression analyses showed that blood pressure and PR were the main influencing factors on C1 and C2. LFHT was an influence on C2 and HFHT on C1. Conclusions Textile mill noise pollution is very serious and has an obvious influence on worker＇s auditory function. The female workers with low artery compliance or with high blood pressure might be suffering from hearing loss; those with noise induced hearing loss might be suffering from hypertension if she is constantly exposed to loud industrial noise.
