AIM： To explore the effects of conditioned media on the proliferation of corneal endothelial cells （CECa） and to compare the efficiency of different conditioned media （CM）. METHODS： Rat CECs, corneal stromal cel...AIM： To explore the effects of conditioned media on the proliferation of corneal endothelial cells （CECa） and to compare the efficiency of different conditioned media （CM）. METHODS： Rat CECs, corneal stromal cells （CSCs）, bone marrow -derived endothelial progenitor cells （BEPCs）, and bone marrow-derived mesenchymal stem cells （BMSCs） were isolated and cultured in vitra CM was collected from CSCs, BEPCs, and BMSCSo CECs were cultivated in different culture media. Cell morphology was recorded, and gene and protein expression were analyzed.~ RESULTS： After grown in CM for 5d, CECs in each experimental group remained polygonal, in a cobblestone- like monolayer arrangement. Immunocytofluorescence revealed positive expression of Na＋/K＋-ATP, aquaporin 1 （AQP1）, and zonula occludens 1 （ZO-1）. Based on quantitative polymerase chain reaction （qPCR） analysis, Na ＋/K ＋-ATP expression in CSC-CM was notably upregulated by 1.3-fold （＋0.036） （P〈0.05, n=3）. The expression levels of ZO-1, neuron specific enolase （NSE）, Vimentin, paired homebox 6 （PAX6）, and procollagen type VII （COL8A1） were notably upregulated in each experimental group. Each CM had a positive effect on CEC proliferation, and CSC-CM had the strongest effect on proliferation.~ CONCLUSION： CSC-CM, BEPC-CM, and BMSC-CM not only stimulated the proliferation of CECs, but also maintained the characteristic differentiated phenotypes necessary for endothelial functions. CSC-CM had the most notable effect on CEC proliferation. KEYWORDS： conditioned medium; corneal endothelial cell; corneal stromal cell; bone marrow-derived endothelial progenitor cell; proliferation展开更多
Extensive acidic soils,which suffer from accelerated soil acidification,are found in southern China.Soil acidity,aluminum toxicity,and nutrient deficiencies severely limited crop productivity in acidic soils.It has be...Extensive acidic soils,which suffer from accelerated soil acidification,are found in southern China.Soil acidity,aluminum toxicity,and nutrient deficiencies severely limited crop productivity in acidic soils.It has been widely reported that crop residue biochars can ameliorate acidic soils and increase crop productivity.Here,we summarized the positive effects and mechanisms involved in the correction of soil acidity,the alleviation of aluminum toxicity and the increase of soil pH buffering capacity by crop residue biochars.The carbonate,oxygen-containing functional groups and silicates in biochars are the major components responsible for their efficacy in amending acidic soils and resisting soil re-acidification.We conclude that application of crop residue biochars may be a better option than traditional liming to ameliorate acidic soils.Nonetheless,further researches into soil acidification are still required to address some issues that are controversial and poorly understood.展开更多
基金Supported by National Nature Science Foundation of China(No.81370992,No.81570812,No. 81500765)Shanghai Municipal Commission of Health and Family Planning For Shanghai Young Doctor Training Program(No.20144Y0221)
文摘AIM： To explore the effects of conditioned media on the proliferation of corneal endothelial cells （CECa） and to compare the efficiency of different conditioned media （CM）. METHODS： Rat CECs, corneal stromal cells （CSCs）, bone marrow -derived endothelial progenitor cells （BEPCs）, and bone marrow-derived mesenchymal stem cells （BMSCs） were isolated and cultured in vitra CM was collected from CSCs, BEPCs, and BMSCSo CECs were cultivated in different culture media. Cell morphology was recorded, and gene and protein expression were analyzed.~ RESULTS： After grown in CM for 5d, CECs in each experimental group remained polygonal, in a cobblestone- like monolayer arrangement. Immunocytofluorescence revealed positive expression of Na＋/K＋-ATP, aquaporin 1 （AQP1）, and zonula occludens 1 （ZO-1）. Based on quantitative polymerase chain reaction （qPCR） analysis, Na ＋/K ＋-ATP expression in CSC-CM was notably upregulated by 1.3-fold （＋0.036） （P〈0.05, n=3）. The expression levels of ZO-1, neuron specific enolase （NSE）, Vimentin, paired homebox 6 （PAX6）, and procollagen type VII （COL8A1） were notably upregulated in each experimental group. Each CM had a positive effect on CEC proliferation, and CSC-CM had the strongest effect on proliferation.~ CONCLUSION： CSC-CM, BEPC-CM, and BMSC-CM not only stimulated the proliferation of CECs, but also maintained the characteristic differentiated phenotypes necessary for endothelial functions. CSC-CM had the most notable effect on CEC proliferation. KEYWORDS： conditioned medium; corneal endothelial cell; corneal stromal cell; bone marrow-derived endothelial progenitor cell; proliferation
基金funded by the National Key Research and Development of China(2016YFD0200302)the National Key Basic Research Program of China(2014CB441003)
文摘Extensive acidic soils,which suffer from accelerated soil acidification,are found in southern China.Soil acidity,aluminum toxicity,and nutrient deficiencies severely limited crop productivity in acidic soils.It has been widely reported that crop residue biochars can ameliorate acidic soils and increase crop productivity.Here,we summarized the positive effects and mechanisms involved in the correction of soil acidity,the alleviation of aluminum toxicity and the increase of soil pH buffering capacity by crop residue biochars.The carbonate,oxygen-containing functional groups and silicates in biochars are the major components responsible for their efficacy in amending acidic soils and resisting soil re-acidification.We conclude that application of crop residue biochars may be a better option than traditional liming to ameliorate acidic soils.Nonetheless,further researches into soil acidification are still required to address some issues that are controversial and poorly understood.
