The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlin...The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlings of V.faba planted in this peat soil were treated with either distilled water(CK)or 25 mg·L^(−1)(G25)of functionalized graphene solution.Results showed that the height and root length of V.faba seedlings in the G25 group were significantly larger than those in CK group.The microbial com-munity was analyzed by amplifying and sequencing the 16S rRNA gene V_(3)-V_(4) region of bacteria and internal transcribed spacer re-gion of fungi in rhizosphere soil using Illumina MiSeq technology.Alpha and beta diversity analysis indicated that functionalized graphene increased the richness and diversity of bacteria and fungi in the V.faba rhizosphere peat soil.The abundances of three ni-trogen cycling-related bacteria,Hydrogenophaga,Sphingomonas and Nitrosomonadaceae,were also altered after treatment with the functionalized graphene.The relative abundance of Basilicum,related to soil phosphorus solubilization,decreased in the fungal com-munity,while the relative abundance of Clonostachys and Dimorphospora,which exhibited strong biological control over numerous fungal plant pathogens,nematodes and insects,increased in the soil after functionalized graphene treatment.Redundancy analysis re-vealed that the potential of hydrogen(pH),organic matter,and total phosphorus contributed the most to the changes in bacterial and fungal community composition in the rhizosphere soil.Overall,our findings suggested that the addition of functionalized graphene altered the relative abundances of nitrogen and phosphorus cycling-related microorganisms in peat soil,promoting changes in the physicochemical properties of the soil and ultimately leading to the improved growth of V.faba plants.展开更多
目的探讨重庆北碚地区中老年男性、女性腰椎骨密度(bone mineral density,BMD)的变化规律,以及脊柱脆性骨折的发病特点。方法本研究选取了两组人群:①常住重庆北碚地区的社区健康人群,年龄40~80岁,共计2126例;②明确诊断为脊柱脆性骨折...目的探讨重庆北碚地区中老年男性、女性腰椎骨密度(bone mineral density,BMD)的变化规律,以及脊柱脆性骨折的发病特点。方法本研究选取了两组人群:①常住重庆北碚地区的社区健康人群,年龄40~80岁,共计2126例;②明确诊断为脊柱脆性骨折的人群,共计137例。两组人群均进行三维定量CT(3D-QCT)BMD测量。将社区健康人群分为中年组(40~59岁)、老年组(60~80岁),比较中老年男性、女性BMD的变化规律;脊柱脆性骨折行CT和(或)MRI检查,至少明确出现一个椎体骨折,MRI明确出现多次骨折,按统计数据绘制ROC曲线,查找脊柱脆性骨折的BMD临界值,分析脊柱脆性骨折的发病特点。结果社区健康人群组:男性、女性的BMD变化与年龄增长呈负相关(r=-0.747、-0.712,P<0.05)。低骨量高发年龄男女性均在50岁以后,女性50~59岁(发生率52.5%),男性50~69岁(发生率50.2%);骨质疏松症(osteoporosis,OP)好发于女性60岁、男性70岁以后。脊柱脆性骨折组:发生于OP人群中女性占83.33%、男性占67.92%;发生于低骨量人群中女性占16.7%、男性占32.1%。多椎体(多次)脆性骨折发生率为31.39%(43/137),其中OP占79.07%、低骨量占20.9%;OP+脆性骨折人群中,多椎体(多次)脆性骨折女性占30%、男性占36.11%;脆性骨折BMD最佳临界值女性为50.05 mg/cm3、男性71.18 mg/cm3。结论脆性骨折高发于OP人群、次发生于低骨量人群,OP+脆性骨折患者较低骨量+脆性骨折患者再骨折的风险大。因此,50岁以后应常规检测BMD,关注低骨量,提前干预OP有助于提高脆性骨折的二级预防。展开更多
文摘The effect of functionalized graphene on the growth and development of Vicia faba L.was investigated by analyzing its impact on the composition and diversity of the microbial community in rhizosphere peat soil.Seedlings of V.faba planted in this peat soil were treated with either distilled water(CK)or 25 mg·L^(−1)(G25)of functionalized graphene solution.Results showed that the height and root length of V.faba seedlings in the G25 group were significantly larger than those in CK group.The microbial com-munity was analyzed by amplifying and sequencing the 16S rRNA gene V_(3)-V_(4) region of bacteria and internal transcribed spacer re-gion of fungi in rhizosphere soil using Illumina MiSeq technology.Alpha and beta diversity analysis indicated that functionalized graphene increased the richness and diversity of bacteria and fungi in the V.faba rhizosphere peat soil.The abundances of three ni-trogen cycling-related bacteria,Hydrogenophaga,Sphingomonas and Nitrosomonadaceae,were also altered after treatment with the functionalized graphene.The relative abundance of Basilicum,related to soil phosphorus solubilization,decreased in the fungal com-munity,while the relative abundance of Clonostachys and Dimorphospora,which exhibited strong biological control over numerous fungal plant pathogens,nematodes and insects,increased in the soil after functionalized graphene treatment.Redundancy analysis re-vealed that the potential of hydrogen(pH),organic matter,and total phosphorus contributed the most to the changes in bacterial and fungal community composition in the rhizosphere soil.Overall,our findings suggested that the addition of functionalized graphene altered the relative abundances of nitrogen and phosphorus cycling-related microorganisms in peat soil,promoting changes in the physicochemical properties of the soil and ultimately leading to the improved growth of V.faba plants.
文摘目的探讨重庆北碚地区中老年男性、女性腰椎骨密度(bone mineral density,BMD)的变化规律,以及脊柱脆性骨折的发病特点。方法本研究选取了两组人群:①常住重庆北碚地区的社区健康人群,年龄40~80岁,共计2126例;②明确诊断为脊柱脆性骨折的人群,共计137例。两组人群均进行三维定量CT(3D-QCT)BMD测量。将社区健康人群分为中年组(40~59岁)、老年组(60~80岁),比较中老年男性、女性BMD的变化规律;脊柱脆性骨折行CT和(或)MRI检查,至少明确出现一个椎体骨折,MRI明确出现多次骨折,按统计数据绘制ROC曲线,查找脊柱脆性骨折的BMD临界值,分析脊柱脆性骨折的发病特点。结果社区健康人群组:男性、女性的BMD变化与年龄增长呈负相关(r=-0.747、-0.712,P<0.05)。低骨量高发年龄男女性均在50岁以后,女性50~59岁(发生率52.5%),男性50~69岁(发生率50.2%);骨质疏松症(osteoporosis,OP)好发于女性60岁、男性70岁以后。脊柱脆性骨折组:发生于OP人群中女性占83.33%、男性占67.92%;发生于低骨量人群中女性占16.7%、男性占32.1%。多椎体(多次)脆性骨折发生率为31.39%(43/137),其中OP占79.07%、低骨量占20.9%;OP+脆性骨折人群中,多椎体(多次)脆性骨折女性占30%、男性占36.11%;脆性骨折BMD最佳临界值女性为50.05 mg/cm3、男性71.18 mg/cm3。结论脆性骨折高发于OP人群、次发生于低骨量人群,OP+脆性骨折患者较低骨量+脆性骨折患者再骨折的风险大。因此,50岁以后应常规检测BMD,关注低骨量,提前干预OP有助于提高脆性骨折的二级预防。
