Nowadays, despite the high prevalence of hypertension, the awareness rate and control rate of this disease are still very low. In addition, the prevalence of hypertension in old population is very high, and most of ca...Nowadays, despite the high prevalence of hypertension, the awareness rate and control rate of this disease are still very low. In addition, the prevalence of hypertension in old population is very high, and most of cases are isolated systolic hypertension （ISH）. We investigated people ranging in age from 80 to 99 years in 28 cadre retirement centers in Beijing to study the prevalence and incidence of target organ injury and analyze the relative factors of ISH.展开更多
Background Stent thrombosis is one of severe complications after sirolimus-eluting stent implantation. Rapamycin （sirolimus） promotes arterial thrombosis in in vivo studies. However, the underlying molecular and tra...Background Stent thrombosis is one of severe complications after sirolimus-eluting stent implantation. Rapamycin （sirolimus） promotes arterial thrombosis in in vivo studies. However, the underlying molecular and transcriptional mechanisms of this adverse effect have not been thoroughly investigated. This study was designed to examine the effects of rapamycin on the expression of the gene, Kruppel-like factor 2 （KLF2）, and its transcriptional targets in mice. Methods Mice were randomly divided into four groups： the control group （intraperitoneal injection with 2.5% of dimethyl sulfoxide （DMSO） only）, rapamycin group （intraperitoneal injection with 2 mg/kg of rapamycin only）, Ad-LacZ ＋ rapamycin group （carotid arterial incubation with Ad-LacZ plus intraperitoneal injection with 2 mg/kg of rapamycin 10 days later）, and Ad-KLF2 ＋ rapamycin group （carotid arterial incubation with Ad-KLF2 plus intraperitoneal injection with 2 mg/kg rapamycin 10 days later）. The carotid arterial thrombosis formation was induced by FeCI3 and the time of arterial thrombosis was determined. Finally, the RNA and protein of carotid arteries were extracted for KLF2, tissue factor （TF）, plasminogen activator inhibitor-1 （PAl-l）, endothelial nitric oxide synthase （eNOS）, thrombomodulin （TM） mRNA and protein analysis. Results Compared with controls, treatment with rapamycin inhibited KLF2, eNOS and TM mRNA and protein expression, and enhanced TF and PAl-1 mRNA and protein expression, and shortened time to thrombotic occlusion from （1282＋347） seconds to （715＋120） seconds （P 〈0.01） in vivo. Overexpression of KLF2 strongly reversed rapamycin-induced effects on KLF2, eNOS, TM, TF and PAl-1 expression. KLF2 overexpression increased the time to thrombotic occlusion to control levels in vivo. Conclusions Rapamycin induced an inhibition of KLF2 expression and an imbalance of anti- and pro-thrombotic gene expression, which promoted arterial thrombosis in vivo. Overexpression of KLF2 increased KLF2 expression and reversed time to thrombosis in vivo.展开更多
文摘Nowadays, despite the high prevalence of hypertension, the awareness rate and control rate of this disease are still very low. In addition, the prevalence of hypertension in old population is very high, and most of cases are isolated systolic hypertension （ISH）. We investigated people ranging in age from 80 to 99 years in 28 cadre retirement centers in Beijing to study the prevalence and incidence of target organ injury and analyze the relative factors of ISH.
基金This siudy waspartly supported by Beijing NOVA Program （No. 2007B002） to Dr. NIE Xiao-min, National Natural Science Foundation of China （No. 81070171）, and Specialized Research Fund for the Doctoral Program of Higher Education of China （No. 20111106110013） to Dr. LI Jian-jun.
文摘Background Stent thrombosis is one of severe complications after sirolimus-eluting stent implantation. Rapamycin （sirolimus） promotes arterial thrombosis in in vivo studies. However, the underlying molecular and transcriptional mechanisms of this adverse effect have not been thoroughly investigated. This study was designed to examine the effects of rapamycin on the expression of the gene, Kruppel-like factor 2 （KLF2）, and its transcriptional targets in mice. Methods Mice were randomly divided into four groups： the control group （intraperitoneal injection with 2.5% of dimethyl sulfoxide （DMSO） only）, rapamycin group （intraperitoneal injection with 2 mg/kg of rapamycin only）, Ad-LacZ ＋ rapamycin group （carotid arterial incubation with Ad-LacZ plus intraperitoneal injection with 2 mg/kg of rapamycin 10 days later）, and Ad-KLF2 ＋ rapamycin group （carotid arterial incubation with Ad-KLF2 plus intraperitoneal injection with 2 mg/kg rapamycin 10 days later）. The carotid arterial thrombosis formation was induced by FeCI3 and the time of arterial thrombosis was determined. Finally, the RNA and protein of carotid arteries were extracted for KLF2, tissue factor （TF）, plasminogen activator inhibitor-1 （PAl-l）, endothelial nitric oxide synthase （eNOS）, thrombomodulin （TM） mRNA and protein analysis. Results Compared with controls, treatment with rapamycin inhibited KLF2, eNOS and TM mRNA and protein expression, and enhanced TF and PAl-1 mRNA and protein expression, and shortened time to thrombotic occlusion from （1282＋347） seconds to （715＋120） seconds （P 〈0.01） in vivo. Overexpression of KLF2 strongly reversed rapamycin-induced effects on KLF2, eNOS, TM, TF and PAl-1 expression. KLF2 overexpression increased the time to thrombotic occlusion to control levels in vivo. Conclusions Rapamycin induced an inhibition of KLF2 expression and an imbalance of anti- and pro-thrombotic gene expression, which promoted arterial thrombosis in vivo. Overexpression of KLF2 increased KLF2 expression and reversed time to thrombosis in vivo.
