尚玺胶囊的抗炎作用及其对血清IL-1β、TNF-α和炎症组织中PGE_(2)的影响

目的评估尚玺胶囊的抗炎作用,探讨其对血清IL-1β、TNF-α和炎症组织中PGE_(2)的影响。方法选择角叉菜胶诱导的大鼠急性炎症模型,测定了大鼠的足周径和足肿胀抑制率。随后,采用生化法测定了血清中超氧化物歧化酶(Superoxide dismutase,SOD)活力、丙二醛(Malondialdehyde,MDA)含量;采用酶联免疫吸附实验(ELISA)检测血清中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)-含量和炎症足爪中前列腺素E_(2)(PGE_(2))含量。结果相较于对照组,给予尚玺胶囊后,炎症足爪肿胀度在1至4小时内显著降低。当尚玺胶囊的给药剂量为300mg·kg^(-1)时,2小时后足肿胀抑制率达到峰值(7.58%),接近于白藜芦醇的抑制率(8.30%);另外,血清中MDA、IL-1β和TNF-α的含量下降、SOD含量升高,炎症组织中PGE_(2)的含量也显著降低。结论尚玺胶囊在300 mg/kg的剂量下能减轻动物的急性炎症反应,其作用机制可能与降低血清IL-1β、TNF-α水平、减少炎症组织中PGE_(2)合成和抑制组织炎症中脂质过氧化产物的形成有关。

野山参中1个新的聚炔类化合物

目的研究野山参Panax ginseng根与根茎的化学成分。方法利用HPD-400大孔吸附树脂和反相硅胶柱色谱以及半制备高效液相色谱等方法进行分离纯化,通过质谱、核磁共振波谱(^(1)H-和^(13)C-NMR以及HSQC、HMBC实验)等手段鉴定化合物结构。结果从野山参70%乙醇提取物经HPD-400大孔吸附树脂90%乙醇洗脱部位分离得到5个化合物,分别鉴定为8Z-癸烷-4,6-二炔-1-O-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃葡萄糖苷(1)、三七皂苷Rt(2)、20(E)-人参皂苷F_(4)(3)、人参皂苷Rg_(6)(4)、20(E)-人参皂苷Rg_(9)(5)。结论化合物1为新的聚炔类化合物,命名为人参炔氧苷A;化合物2~5为首次从野山参中分离得到。

Research progress on naturally-occurring and semi-synthetic ocotillol-type ginsenosides in the genus Panax L.(Araliaceae)

Ocotillol(OT)-type ginsenosides,one subtype of ginsenosides,consist of a dammarane skeleton and a tetrahydrofuran ring.Most naturally-occurring OT-type ginsenosides exist in Panax species,particularly in Panax quinquefolius,which may be attributed to the warm and humid climate of its native areas.Till now,merely 28 types of naturally-occurring OT-type ginsenosides have been isolated.In contrast,semi-synthesized OT-type ginsenosides are attracted considerable attentions.These ginsenosides can be obtained through oxidation and cyclization of side chains of dammarane-type ginsenosides,and other methods,which may change their physical and chemical properties and further improve their bioavailabilities.It is also notable that the pharmacological activities of ginsenosides are closely related to the stereoisomers caused by the configuration at C-20.Semi-synthesis of OT-type ginsenosides can facilitate our understanding of the biosynthesis,transformation and metabolism of OT-type ginsenosides in the body.This review will systematically summarize the research progress on naturally-occurring and semi-synthetic OT-type ginsenosides,which provides a theoretical basis for their bioactivity-guided research.