Comparative analysis of the microbiome of sympatric wintering Bean Geese,Domestic Ducks,humans,and soil at Shengjin Lake of China reveals potential public risk to human health

The gut microbiota of migratory waterbirds is affected by various complex factors,including cross-species transmission,which increases the risk of pathogen spreading among sympatric animals and poses a potential public health risk to humans.In this study,we investigated the microbial communities of wintering Bean Geese(Anser fabalis),Domestic Ducks(A.platyrhynchos domesticus),humans,and soil using high-throughput sequencing of the 16S rRNA gene region in Shengjin Lake,China.In total,6,046,677 clean reads were obtained,representing 41,119 operational taxonomic units(OTUs)across the four groups.The dominant microbial phyla were the Proteobacteria,Firmicutes,Bacteroidota,and Actinobacteriota.The Sorensen similarity index and alpha and beta diversity results showed that the gut microbial communities of Bean Geese and Domestic Ducks were more similar to those of the other pairs.Network analysis revealed that Faecalibacterium prausnitzii,Pseudomonas fragi,and Bradyrhizobium elkanii were hubs of the three major modules.Fourteen common microbiomes were iden-tified in Bean Geese,Domestic Ducks,humans,and soil in Shengjin Lake.A total of 96 potential pathogens were identified among the four groups,with 20 specific potentially pathogenic microbiomes found in the gut of Bean Geese.Some of these pathogens are responsible for significant financial losses in the poultry industry and pose risks to human health.Klebsiella pneumoniae,Morganella morganii,Escherichia coli,and Ralstonia insidiosa are potential core pathogens found in the four groups at Shengjin Lake that can cause diseases in humans and an-imals and facilitate cross-species transmission through various media.Therefore,humans are at risk of con-tracting these pathogens from migratory birds because of their frequent contact with domestic poultry.However,further studies are required to explore the potential pathogenic species and transmission pathways among sympatric wintering Bean Geese,Domestic Ducks,humans,and soil.

Effects of PEAR1 gene polymorphism on big endothelin-1 levels in Chinese patients with acute myocardial infarction after percutaneous coronary intervention

Acute myocardial infarction (AMI) has been associated with poor prognosis,even after revascularization with percutaneous coronary intervention (PCI),likely due to coronary endothelial cell dysfunction and injury.^([1,2])Endothelin-1 (ET-1),a peptide that serves as a vasoconstrictor of smooth muscle cell proliferation,can reflect endothelial cell functional states.Due to low circulation levels and short plasma half-life time,measuring plasma ET-1 levels is difficult.In contrast,big ET-1.

A cloud optical and microphysical property product for the advanced geosynchronous radiation imager onboard China's Fengyun-4 satellites: The first version

风云四号作为中国新一代静止气象卫星,提供了高时空分辨率的监测产品。本文介绍风云四号搭载的先进地球同步轨道辐射成像仪AGRI的云光学和微物理特性产品.该产品包含了基于双光谱通道反演的云光学厚度和云粒子有效半径产品,以及基于机器学习的云识别和云相态产品。与时空匹配的主动卫星观测结果对比显示,该产品的云识别和云相态的准确率分别在95%和85%;该产品提供的云光学厚度和云有效粒径与经典的MODIS产品的相关系数达到0.76和0.63.团队将持续优化和更新该云光学和微物理特性定量产品,服务风云四号卫星定量应用。

Alleviation of the plastic deformation of gel ink under strong stress through an esterification of xanthan gum reinforcing its double helix structure

As a natural organic polymer,xanthan gum(XG)can alleviate the plastic deformation of gel ink under strong stress and realize the reasonable regulation of the rheological properties of gel ink.However,as the double-helix structure connected by hydrogen bonds cannot resist the mechanical environment of strong stress,XG shows poor shear resistance.In this study,a polymer gel with interpenetrating polymer network structure was prepared by esterifying XG,taking polystyrene maleic anhydride(SMA)as the modifier.In addition to retaining the excellent rheological properties of XG,the generated polymer gel also exhibited high shear resistance.The optimal addition amount of the esterification reaction modifier was determined as mXG:mSMA=5:3 according to the gel ink standard.With this amount,the viscosity of the modified xanthan gum(SXG)gel increased to 1578.8 mPa·s and 100.7 mPa·s at shear rates of 4 s1 and 383 s1,respectively,and the shear resistance increased more than 2 times compared to the unmodified one.It is because of the ester bond formed by esterification that the reaction strengthens the interaction between molecular segments,enabling the new gel to resist to strong mechanical stress.The new polymer gel studied in this paper and the proposed mechanism of action provide new insights for the development of high-end gel ink and also provide theoretical support for the study of rheological properties of non-Newtonian fluids.

Investigation on taste characteristics and sensory perception of soft-boiled chicken during oral processing based on electronic tongue and electronic nose

The sensory perception of food is a dynamic process,which is closely related to the release of flavor substances during oral processing.It’s not only affected by the food material,but also subjected to the individual oral environment.To explore the oral processing characteristics of soft-boiled chicken,the sensory properties,texture,particle size,viscosity,characteristic values of electronic nose and tongue of different chicken samples were investigated.The correlation analysis showed that the physical characteristics especially the cohesiveness,springiness,resilience of the sample determined oral processing behavior.The addition of chicken skin played a role in lubrication during oral processing.The particle size of the bolus was heightened at the early stage,and the fluidity was enhanced in the end,which reduced the chewing time to the swallowing point and raised the aromatic compounds signal of electronic nose.But the effect of chicken skin on chicken thigh with relatively high fat content,was opposite in electronic nose,which had a certain masking effect on the perception of umami and sweet taste.In conclusion,fat played a critical role in chicken oral processing and chicken thigh had obvious advantages in comprehensive evaluation of soft-boiled chicken,which was more popular among people.

FY-3E:The First Operational Meteorological Satellite Mission in an Early Morning Orbit

Fengyun-3 E(FY-3E),the world’s first early-morning-orbit meteorological satellite for civil use,was launched successfully at the Jiuquan Satellite Launch Center on 5 July 2021.The FY-3E satellite will fill the vacancy of the global early-morning-orbit satellite observation,working together with the FY-3C and FY-3D satellites to achieve the data coverage of early morning,morning,and afternoon orbits.The combination of these three satellites will provide global data coverage for numerical weather prediction(NWP)at 6-hour intervals,effectively improving the accuracy and time efficiency of global NWP,which is of great significance to perfect the global earth observing system.In this article,the background and meteorological requirements for the early-morning-orbit satellite are reviewed,and the specifications of the FY-3E satellite,as well as the characteristics of the onboard instrumentation for earth observations,are also introduced.In addition,the ground segment and the retrieved geophysical products are also presented.It is believed that the NWP communities will significantly benefit from an optimal temporal distribution of observations provided by the early morning,mid-morning,and afternoon satellite missions.Further benefits are expected in numerous applications such as the monitoring of severe weather/climate events,the development of improved sampling designs of the diurnal cycle for accurate climate data records,more efficient monitoring of air quality by thermal infrared remote sensing,and the quasicontinuous monitoring of the sun for space weather and climate.

Inhibition of SW620 human colon cancer cells by upregulating mi RNA-145

AIM: To investigate the targeted inhibition of proliferation and migration of SW620 human colon cancer cells by upregulating mi RNA-145(mi R-145).METHODS: Forty-five samples of colon cancer tissues and 45 normal control samples were obtained from the biological database of the First Affiliated Hospital of Liaoning Medical University. We performed quantitative analysis of mi R-145 and N-ras expression in tissues; reverse transcriptase polymerase chain reaction analysis of mi R-145 expression in SW620 colon cancer cells and normal colonic epithelial cells; construction of mi R-145 lentiviral vector and determination of mi R-145 expression in SW620 cells transduced with mi R-145 vector; analysis of the effect of mi R-145 overexpression on SW620 cell proliferation; analysis of the effect of mi R-145 overexpression on SW620 cell migration using a wound healing assay; and analysis of the effect ofmi R-145 on N-ras expression using Western blotting. RESULTS: mi R-145 expression was significantly downregulated in colon cancer tissues, with its expression in normal colonic tissues being 4-5-fold higher(two sample t test, P < 0.05), whereas N-ras expression showed the opposite trend. mi R-145 expression in SW620 cells was downregulated, which was significantly lower compared to that in colonic epithelial cells(two sample t test, P < 0.05). mi R-145 vector and control were successfully packaged; expression of mi R-145 in SW620 cells transduced with mi R-145 was 8.2-fold of that in control cells(two sample t test, P < 0.05). The proliferation of mi R-145-transduced SW620 cells was significantly decreased compared to control cells(two sample t test, P < 0.05). At 48 h in the wound healing experiment, the migration indexes and controls were(97.27% ± 9.25%) and(70.22% ± 6.53%), respectively(two sample t test, P < 0.05). N-ras expression in mi R-145-tranduced SW620 cells was significantly lower than others(one-way analysis of variance, P < 0.05). CONCLUSION: mi R-145 is important in inhibiting colon cancer cell proliferation and migration. This is a good foundation for development of colon cancer therapy by targeting tumor suppressor mi R-145.

Facile Synthesis of FePS3 Nanosheets@MXene Composite as a High-Performance Anode Material for Sodium Storage

Searching for advanced anode materials with excellent electrochemical properties in sodium-ion battery is essential and imperative for next-generation energy storage system to solve the energy shortage problem.In this work,two-dimensional(2D)ultrathin FePS3 nanosheets,a typical ternary metal phosphosulfide,are first prepared by ultrasonic exfoliation.The novel 2D/2D heterojunction of FePS3 nanosheets@MXene composite is then successfully synthesized by in situ mixing ultrathin MXene nanosheets with FePS3 nanosheets.The resultant FePS3 nanosheets@MXene hybrids can increase the electronic conductivity and specific surface area,assuring excellent surface and interfacial charge transfer abilities.Furthermore,the unique heterojunction endows FePS3 nanosheets@MXene composite to promote the diffusion of Na^+ and alleviate the drastic change in volume in the cyclic process,enhancing the sodium storage capability.Consequently,the few-layered FePS3 nanosheets uniformly coated by ultrathin MXene provide an exceptional reversible capacity of 676.1 mAh g^−1 at the current of 100 mA g^−1 after 90 cycles,which is equivalent to around 90.6% of the second-cycle capacity(746.4 mAh g^−1).This work provides an original protocol for constructing 2D/2D material and demonstrates the FePS3@MXene composite as a potential anode material with excellent property for sodium-ion batteries.

Effects of chronic obstructive pulmonary disease on longterm prognosis of patients with coronary heart disease postpercutaneous coronary intervention

BACKGROUND Chronic obstructive pulmonary disease(COPD)and cardiovascular diseases are often comorbid conditions,their co-occurrence yields worse outcomes than either condition alone.This study aimed to investigate COPD impacts on the five-year prognosis of patients with coronary heart disease(CHD)after percutaneous coronary intervention(PCI).METHODS Patients with CHD who underwent PCI in 2013 were recruited,and divided into COPD group and non-COPD group.Adverse events occurring among those groups were recorded during the five-year follow-up period after PCI,including all-cause death and cardiogenic death,myocardial infarction,repeated revascularization,as well as stroke and bleeding events.Major adverse cardiac and cerebral events were a composite of all-cause death,myocardial infarction,repeated revascularization and stroke.RESULTS A total of 9843 patients were consecutively enrolled,of which 229 patients(2.3%)had COPD.Compared to non-COPD patients,COPD patients were older,along with poorer estimated glomerular filtration rate and lower left ventricular ejection fraction.Five-year follow-up results showed that incidences of all-cause death and cardiogenic death,as well as major adverse cardiac and cerebral events,for the COPD group were significantly higher than for non-COPD group(10.5%vs.3.9%,7.4%vs.2.3%,and 30.1%vs.22.6%,respectively).COPD was found under multivariate Cox regression analysis,adjusted for confounding factors,to be an independent predictor of all-cause death[odds ratio(OR)=1.76,95%CI:1.15-2.70,P=0.009]and cardiogenic death(OR=2.02,95%CI:1.21-3.39,P=0.007).CONCLUSIONS COPD is an independent predictive factor for clinical mortality,in which CHD patients with COPD are associated with worse prognosis than CHD patients with non-COPD.

Rapid europium-sensitized fluorescent determination of ulifloxacin,the active metabolite of prulifloxacin,in human serum and urine

A new fluorescent method was developed based on the ulifloxacin-europium（Ⅲ）-sodium dodecylbenzene sulfonate system for the determination of ulifloxacin,the active metabolite of prulifloxacin.Sodium dodecylbenzene sulfonate formed a ternary complex with ulifloxacin-europium（Ⅲ）and significantly enhanced the characteristic fluorescence of europium（Ⅲ）.The enhanced fluorescence intensity showed a good linear relationship with the concentration of ulifloxacin in the range of 5.0×10^-8-2.0×10^-6M with a detection limit of 2.0×10^-10 M（3σ）.This method is rapid and sensitive,and has been successfully applied to the determination of ulifloxacin in human urine and serum samples.

Using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to analyze differentially expressed brain polypeptides in scrapie strain 22L-infected BALB/c mice

Differentialiy expressed polypeptides in the brain of a BALB/c mouse model infected with scrapie strain 22L were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results showed that 21 peptides were down-regulated, with peptides of mass-to-charge ratio 758.772 5 and mass-to-charge ratio 5 432.206 9, demonstrating the most significant decreases. These finding suggest that these peptides are candidate biomarkers and may play an important role in the pathogenesis of prion disease.

Effects of monoclonal antibodies against human stathmin 1 combined paclitaxel on proliferation of human hepatocellular carcinoma cell lines

Objective： We investigated the effects of monoclonal antibodies against stathmin 1 combined paclitaxel on the proliferation of HCC cells. Methods： HepG2 cells were treated with monoclonal antibodies against stathmin 1, paclitaxel alone or their combination, with the untreated cells used as the control, 24, 48, 72, 96 h later, the cell growth condition was observed by invert microscope and inhabitation rate was studied by MTT assay; The apoptosis was analyzed by flow cytometry with Annexin V/PI. Results： The population decreased and shape, size changed after treating with different concentration of experimental groups. Monoclonal antibodies against stathmin 1 and paclitaxel used alone or in combination both inhibited the proliferation of HepG2 cells, the inhibition ratio of their combination was more higher （P 〈 0.05）, and a synergistic effect of the two agents was noted in their combined action （P 〈 0.05）. Combined treatment of the cells resulted in significantly higher apoptosis rate than that in the other groups （P 〈 0.05）. Conclusion： Monoclonal antibodies against stathmin 1 and paclitaxel used alone or in combination both can inhibit proliferation of HepG2 cells and induce apoptosis. A synergistic effect is obsewed between the monoclonal antibodies against stathmin 1 and paclitaxel in their inhibition of HepG2 cell proliferation.

Usefulness of white blood cell count to mean platelet volume ratio for predicting long-term prognosis after primary percutaneous coronary intervention in patients with acute coronary syndrome

Background and Objective The white blood cell count to mean platelet volume ratio(WMR)has recently been described as a predictor of cardiovascular events in patients who undergo percutaneous coronary intervention(PCI).The aim of this study was to investigate the usefulness of admission WMR in predicting outcomes in patients with acute coronary syndrome(ACS).

Functional-selected LiF-intercalated-graphene enabling ultra-stable lithium sulfur battery

Using a functionally selective solid electrolyte interphase(SEI)as an anodic protection layer can effectively avoid the subsequent settlement of uneven lithium electrodeposits for lithium sulfur(Li-S)batteries.To address the issues of single functional,mechanical crushing and peeling of the conventional rigid LiF SEI,a unique functional-selected rigid-flexible-coupled LiF-intercalated-graphene(LiF-GN)SEI as anodic protection is constructed,which is verified by in-operando X-ray photoelectron spectroscopy(XPS)spectra.Owing to the synergistic effect of the LiF and graphene layer,this intercalated functionalselected SEI architecture exhibits a dramatic elastic modulus(rigid-flexible coupling with a shallow Young’s modulus(~430 MPa)and a tremendous Young’s modulus of~20 GPa),high mechanical strength,and can be repulsive to polysulfides,accompanied unprecedented trafficability of Li ions.Consequently,the forceful exclusion of polysulfides from the LiF-GN SEI,as confirmed by means of in-situ UV/vis analysis,Li2 S nucleation tests,and visual permeation experiments,is of profound significance for the effective protection of Li anodes and enables Li-S batteries to achieve remarkable electrochemical performance(ultralow capacity decay rate of 0.022%during 300 cycles at 1 C and high discharge capacity of 1092 mAh/g at 0.5 C).

A Computational Study on Lateral Flight Stability of the Cranefly in Hover

The dynamic flight stability of hovering insects includes the longitudinal and lateral motion.Research results have shown that for the majority of hovering insects the same longitudinal natural modes are identified and the hovering flight in longitudinal is unstable.However,in lateral,the modal structure for hovering insects could be different and the stability property of lateral disturbance motion is not as robust as that of longitudinal motion.The cranefly possesses larger aspect ratio and lower Reynolds number,and such differences in morphology and kinematics may make the lateral dynamic stability different.In this paper,the lateral flight stability of the cranefly in hover is investigated by numerical simulation.Firstly,the stability derivatives are acquired by solving the incompressible Navier–Stokes equations.Subsequently,the dynamic stability characteristics are checked by analyzing the eigenvalues and eigenvectors of the linearized system.Computational results indicate that the lateral dynamic modal structure of cranefly is different from most other insects,consisting of three natural modes,and the weakly oscillatory mode illustrates the hovering lateral flight is nearly neutral.This neutral stability is mainly caused by the negative derivative of roll-moment vs.sideslip-velocity,which can be attributed to the weaker‘changingLEV-axial-velocity’effect.These results suggest that insects in nature may exhibit different dynamic stabilities with different morphological and kinematic parameters,which should be considered in the designs of flapping wing air vehicles.

The Relationship between lipoprotein(a) and clinical outcomes in ST elevation myocardial infarction underwent percutaneous coronary intervention

Background and Objective Elevated serum levels of lipoprotein(Lp[a]) has recently been proposed as a marker of cardiovascular risk.We aimed to determine the role of Lp(a) on long-term clinical outcomes in patients with ST elevation myocardial infarction (STEMI) after percutaneous coronary intervention (PCI).

Analyses of therapeutic effects using rludarabine and cytarabine on acute myeloid leukemia at different stages during treatment

Objective: To evaluate the therapeutic effect of the fludarabine and cytarabine (FA) regimen on acute myeloid leukemia (AML) at different phases during treatment. Methods: A total of 185 patients with AML were divided into 4 groups based on the outcome of previous treatments. Patients in Group 1 had no remission after the first course of induction chemotherapy (n = 55). Patients in Group 2 had no remission after no less than two courses of induction chemotherapy (n = 41). Patients in Group 3 had early relapse (n = 40). Patients in Group 4 had late relapse (n = 49). Patients in groups 2, 3 and 4 had refractory AML or AML with relapse. We assessed the efficacy and toxicity of FA combination chemotherapy in each of these 4 groups. Results: The complete remission (CR) rates of Groups 1, 2, 3 and 4 were 74.5% (41/55), 45.9% (19/41), 17.5% (7/40) and 38.8% (19/49), respectively. The CR rate was higher in Group 1 than in the other 3 groups (34.6%, 45/130) (P = 0.000). A significant correlation was found between CR rate and the number of chemotherapeutic courses (P = 0.023). The main adverse reactions included bone marrow suppression and secondary infection. Conclusion: FA regimen is a good choice for patients with AML, especially those who have failed to achieve CR after the first course of induction chemotherapy.

Effects of post-mortem aging process on characteristic water-soluble taste-active precursors in yellow-feathered broilers

Chilled chicken has become the mainstream of chicken consumption.In order to explore the effect of post-mortem aging on water-soluble flavor precursors of chicken,pH,adenosine triphosphate(ATP)degradation,flavor nucleosides,free amino acids and water-soluble low molecular weight peptides were determined using Qingyuan partridge yellow-feathered broilers as material during 0-4℃ post-mortem aging in 48 h.The results showed that the pH value fell to the limit pH 5.64(4 h)in chicken breast and 6.21(3 h)in thigh.Regardless of chicken breast or thigh,ATP dropped rapidly within 3 h.It was found that the K-value in chicken thigh was the lowest at 2 h indicating the freshness was the best.Considering the equivalent umami concentration(EUC),the value at 3 h and 4 h was relatively high,but the corresponding electronic tongue umami value was not high,which further showed that the water-soluble low molecular taste peptide played an important role on the post-mortem aging process.Combined with cluster analysis and partial least squares discriminant analysis(PLS-DA),it was preliminarily inferred that the optimal time for chilled chicken during 0-4℃ post-mortem aging was 2 h,which could provide a theoretical basis for the further processing of fresh chicken.

Dynamically lithium-compensated polymer artificial SEI to assist highly stable lithium-rich manganese-based anode-free lithium metal batteries

Owing to the unique structure,anode-free lithium metal batteries(AFLMBs)have higher energy density and lower production cost than traditional lithium metal batteries(LMBs)or lithium-ion batteries(LIBs),However,AFLMBs suffer from an inherently finite Li reservoir and exhibit poor cycle stability,low Coulombic efficiency(CE)and severe dendrite growth.In this work,polydiallyl lithium disulfide(PDS-Li)was successfully synthesized and coated on Cu current collector by electrochemical polymerization.The PDS-Li acts as an additional lithium resource to compensate for the irreversible loss of lithium during cycling.In addition,the special structure and lithiophilicity of PDS-Li contribute to lower nucleation overpotential and uniform lithium deposition.When coupled with Li-rich manganese-based(LRM)cathode of Li1.2Mn0.54Ni0.13Co0.13O2,the anode-free full cell exhibits significantly improved cycle stability over 100 cycles and capacity retention of 63.3%and 57%after 80 and 100 cycles,respectively.We believe that PDS-Li can be used to ensure stable cycling performance and high-energy-density in AFLMBs.