Research and Practice of Cardiopulmonary Rehabilitation Training Programs

In today’s society, the incidence of cardiopulmonary diseases is increasing annually, seriously affecting patients’ quality of life. Therefore, developing a scientific and effective rehabilitation training program is of great significance. This study first analyzes the theoretical basis of cardiopulmonary rehabilitation training, including the effects of aerobic exercise, interval training, and strength training on cardiopulmonary function. Based on this, a comprehensive rehabilitation training program is designed, which includes personalized training plans, comprehensive interventions, multidisciplinary collaboration, patient education, and regular follow-up visits. The cardiopulmonary rehabilitation training plan developed in this study has certain scientific practicability, which provides a theoretical basis for cardiopulmonary rehabilitation training, and also provides a reference for medical institutions, rehabilitation centers and communities, which is helpful for promotion and application to a wider range of patients with cardiopulmonary diseases.

Intervention Effect of Lower Limb Rehabilitation Robot with Task-Oriented Training on Stroke Patients and Its Influence on KFAROM Score

Objective: To explore the effect of lower limb rehabilitation robot combined with task-oriented training on stroke patients and its influence on KFAROM score. Methods: 100 stroke patients with hemiplegia admitted to our hospital from January 2023 to December 2023 were randomly divided into two groups, the control group (50 cases) was given task-oriented training assisted by nurses, and the observation group (50 cases) was given lower limb rehabilitation robot with task-oriented training. Lower limb balance, lower limb muscle strength, motor function, ankle function, knee flexion range of motion and walking ability were observed. Results: After treatment, the scores of BBS, quadriceps femoris and hamstrings in the observation group were significantly higher than those in the control group (P Conclusion: In the clinical treatment of stroke patients, the combination of task-oriented training and lower limb rehabilitation robot can effectively improve the lower limb muscle strength, facilitate the recovery of balance function, and have a significant effect on the recovery of motor function, which can improve the walking ability of stroke patients and the range of motion of knee flexion, and achieve more ideal therapeutic effectiveness.

The occurrence characteristics of oil in shales matrix from organic geochemical screening data and pore structure properties:An experimental study Author links open overlay panel

The occurrence characteristics of shale oil are of great significance to the movability of shale oil.In this study,the occurrence characteristics of oil in the shale matrix at Funing Formation shale in Subei Basin were quantitatively evaluated by organic geochemistry and microscopic pore structure characterization experiments.The Multiple Isothermal Stages Pyrolysis(MIS)experiment results show that the content of total oil,adsorbed oil,and free oil in the shales are 3.15-11.25 mg/g,1.41-4.95 mg/g,and 1.74-6.51 mg/g,respectively.among which the silicon-rich shale has the best oil-bearing.The relative content of free oil shows an increasing trend in pores with pore diameters greater than 3 nm.When the relative content of free oil reaches 100%,the pore size of silicon-rich shale is about 200 nm,while that of calcium-rich shale,clay-rich shale,and siliceous mixed shale is about 10 nm.The occurrence law of adsorbed oil is opposite to that of free oil,which indicates that shale oil will occur in the pores and fractures in a free state in a more extensive pore size range(>200 nm).This study also enables us to further understand the occurrence characteristics of shale oil under the interaction of occurrence state and occurrence space.

Boosting Chemodynamic Therapy by the Synergistic Effect of Co‑Catalyze and Photothermal Effect Triggered by the Second Near‑Infrared Light

In spite of the tumor microenvironments responsive cancer therapy based on Fenton reaction(i.e.,chemodynamic therapy,CDT)has been attracted more attentions in recent years,the limited Fenton reaction efficiency is the important obstacle to further application in clinic.Herein,we synthesized novel FeO/MoS2 nanocomposites modified by bovine serum albumin(FeO/MoS2-BSA)with boosted Fenton reaction efficiency by the synergistic effect of co-catalyze and photothermal effect of MoS2 nanosheets triggered by the second near-infrared(NIR II)light.In the tumor microenvironments,the MoS2 nanosheets not only can accelerate the conversion of Fe3+ions to Fe2+ions by Mo4+ions on their surface to improve Fenton reaction efficiency,but also endow FeO/MoS2-BSA with good photothermal performances for photothermal-enhanced CDT and photothermal therapy(PTT).Consequently,benefiting from the synergetic-enhanced CDT/PTT,the tumors are eradicated completely in vivo.This work provides innovative synergistic strategy for constructing nanocomposites for highly efficient CDT.

Neonatal rhesus monkeys as an animal model for rotavirus infection

AIM To establish a rotavirus(RV)-induced diarrhea model using RV SA11 in neonatal rhesus monkeys for the study of the pathogenic and immune mechanisms of RV infection and evaluation of candidate vaccines.METHODS Neonatal rhesus monkeys with an average age of 15-20 d and an average weight of 500 g ± 150 g received intragastric administration of varying doses of SA11 RV( 107 PFUs/mL, 106 PFUs/mL, or 105 PFUs/mL, 10 mL/animal) to determine whether the SA11 strain can effectively infect these animals by observing their clinical symptoms, fecal shedding of virus antigen by ELISA, distribution of RV antigen in the organs by immunofluorescence, variations of viral RNA load in the organs by qRT-PCR, histopathological changes in the small intestine by HE staining, and apoptosis of small intestinal epithelial cells by TUNEL assay.RESULTS The RV monkey model showed typical clinical diarrhea symptoms in the 108 PFUs SA11 group, where we observed diarrhea 1-4 d post infection(dpi) and viral antigen shed in the feces from 1-7 dpi. RV was found in jejunal epithelial cells. We observed a viral load of approximately 5.85 × 103 copies per 100 mg in the jejunum at 2 dpi, which was increased to 1.09 × 105 copies per 100 mg at 3 dpi. A relatively high viral load was also seen in mesenteric lymph nodes at 2 dpi and 3 dpi. The following histopathological changes were observed in the small intestine following intragastric administration of SA11 RV: vacuolization, edema, and atrophy. Apoptosis in the jejunal villus epithelium was also detectable at 3 dpi.CONCLUSION Our results indicate that we have successfully established a RV SA11 strain diarrhea model in neonatal rhesus monkeys. Future studies will elucidate the mechanisms underlying the pathogenesis of RV infection, and we will use the model to evaluate the protective effect of candidate vaccines.

Isolation and characterization of a new candidate human inactivated rotavirus vaccine strain from hospitalized children in Yunnan,China:2010-2013

AIM To determine the distribution of rotavirus VP7 gene in hospitalized children in Yunnan, China. METHODS A total of 366 stool specimens were collected from hospitalized children in hospitals in Yunnan Province from September 2010 to December 2013. The genomic RNA electropherotypes and the G genotypes of the rotaviruses were determined. A phylogenetic analysis of the VP7 gene was performed. Rotavirus isolation was performed, and characterized by plaque, minimum essential medium, and all genes sequence analysis. Quantification of antibodies for inactivated vaccine prepared with ZTR-68 was examined by enzyme-linked immunosorbent assay and microneutralization assay.RESULTS Group A human rotavirus was detected in 177 of 366(48.4%) stool samples using a colloidal gold device assay. The temporal distribution of rotavirus cases showed significant correlation with the mean air temperature. Rotaviruses were isolated from 13% of the rotavirus-positive samples. The predominant genotype was G1(43.5%), followed by G3(21.7%), G9(17.4%), G2(4.3%), G4(8.7%), and mixed(4.3%) among a total of 23 rotavirus isolates. A rotavirus strain was isolated from a rotavirus-positive stool sample of a 4-month-old child in The First People's Hospital of Zhaotong(2010) for use as a candidate human inactivated rotavirus vaccine strain and for further research, and was designated ZTR-68. The genotype of 11 gene segments of strain ZTR-68(RVA/Human-wt/CHN/ZTR-68/2010/G1P[8]) was characterized. The genotype constellation of strain ZTR-68 was identified as G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. The VP7 and VP4 genotypes of strain ZTR-68 were similar to Wa-like strains.CONCLUSIONS A high prevalence of the G1, G2, and G3 genotypes was detected from 2010 to 2012. However, a dominant prevalence of the G9 genotype was identified as the cause of gastroenteritis in children in Yunnan, China, in 2013. A candidate human inactivated rotavirus vaccine strain, designated ZTR-68 was isolated, characterized, and showed immunogenicity. Our data will be useful for the future formulation and development of a vaccine in China.

Liposome cocktail activator modulates hepatocytes and remodels the microenvironment to mitigate acute liver failure

Acute liver failure(ALF)is a mortal and critical hepatic disease,in which oxidative stress,inflammation storm and hepatocyte death are crucial in the pathogenesis.Hence,in contrast to the control of a single link,a combination therapy targeting multiple pathogenic links of the disease will be a favorable means to control the progression of the disease.In this study,we constructed dimethyl itaconate-loaded liposomes modified with dodecyl gallate as a cocktail activator to investigate its functional role in acetaminophen(APAP)-induced ALF.Our results demonstrated that the cocktail activator acted on hepatocytes and triggered cocktail efficacy,thereby simultaneously attenuating APAP-induced hepatocyte damage and remodeling the damage microenvironment.The cocktail activator could effectively scavenge reactive oxygen species,inhibit excessive inflammatory responses and reduce cell death in impaired hepatocytes for detoxification.More importantly,the cocktail activator could remodel the damage microenvironment,thus further promoting hepatocyte expansion and specifically switching macrophages from the M1 to M2 phenotype for a favorable liver regeneration of ALF.Furthermore,in APAP-induced ALF mouse model,the cocktail activator improved liver function,alleviated histopathological damage and increased survival rate.In summary,these findings indicate that the cocktail activator may provide a promising therapeutic approach for ALF treatment as a nanomedicine.

A Phase Estimation Algorithm for Quantum Speed-Up Multi-Party Computing

Security and privacy issues have attracted the attention of researchers in the field of IoT as the information processing scale grows in sensor networks.Quantum computing,theoretically known as an absolutely secure way to store and transmit information as well as a speed-up way to accelerate local or distributed classical algorithms that are hard to solve with polynomial complexity in computation or communication.In this paper,we focus on the phase estimation method that is crucial to the realization of a general multi-party computing model,which is able to be accelerated by quantum algorithms.A novel multi-party phase estimation algorithm and the related quantum circuit are proposed by using a distributed Oracle operator with iterations.The proved theoretical communication complexity of this algorithm shows it can give the phase estimation before applying multi-party computing efficiently without increasing any additional complexity.Moreover,a practical problem of multi-party dating investigated shows it can make a successful estimation of the number of solution in advance with zero communication complexity by utilizing its special statistic feature.Sufficient simulations present the correctness,validity and efficiency of the proposed estimation method.

Collusion detector based on G-N algorithm for trust model

In the open network environment, malicious attacks to the trust model have become increasingly serious. Compared with single node attacks, collusion attacks do more harm to the trust model. To solve this problem, a collusion detector based on the GN algorithm for the trust evaluation model is proposed in the open Internet environment. By analyzing the behavioral characteristics of collusion groups, the concept of flatting is defined and the G-N community mining algorithm is used to divide suspicious communities. On this basis, a collusion community detector method is proposed based on the breaking strength of suspicious communities. Simulation results show that the model has high recognition accuracy in identifying collusion nodes, so as to effectively defend against malicious attacks of collusion nodes.

不同暗瞳直径屈光不正患者ICL植入术后视觉质量比较

目的:比较不同暗瞳直径下有晶状体眼后房型人工晶状体(ICL)植入术后的视觉质量。方法:前瞻性临床研究。连续选取2022年10月至2023年6月在东莞爱尔眼科医院屈光手术中心行ICL V4c植入手术的屈光不正患者90例(90眼)。根据暗瞳直径分为3组:A组≤6.5 mm,B组>6.5~7.3 mm,C组>7.3 mm,每组均30例(30眼),均选取右眼数据。观察并记录患者术前、术后3个月的裸眼视力(UCVA)、等效球镜度(SE)。使用OPD-ScanⅢ像差分析仪测量4 mm和7 mm瞳孔直径下的客观视觉质量参数,包括全眼高阶像差的Zenike多项式的均方根(RMS)值、斯特列尔比(SR)、调制传递函数(MTF)曲线与正常眼区域之间的比例(AR),其中全眼高阶像差包括总高阶像差(HOAs)、彗差、三叶草像差、球差。组内手术前后参数比较采用配对t检验或秩和检验,组间数据比较采用单因素方差分析或Kruskal-Wallis检验。术前暗瞳直径与AR的相关性采用Pearson相关检验分析,与余参数的相关性分析采用Spearman秩相关检验。结果:A、B、C组组内术后3个月的UCVA、SE均较术前改善(均P<0.001)。在4 mm瞳孔直径下,3组组内手术前后高阶像差比较差异均无统计学意义(均P>0.05);在7 mm瞳孔直径下,A组术后HOAs、三叶草像差与术前差异有统计学意义(Z=-2.37,P=0.018;t=-2.59,P=0.015),B组和C组术后HOAs、彗差、三叶草像差、球差与术前比较差异均有统计学意义(均P<0.001)。在7 mm瞳孔直径下,3组组间ICL植入术后的HOAs、彗差、球差及其变化值差异均有统计学意义(H=31.74~56.71,均P<0.001);在4 mm瞳孔直径下,以上指标及其变化值差异均无统计学意义(均P>0.05)。在4 mm和7 mm瞳孔直径下,术后3个月3组组内SR、AR均较术前明显升高(均P<0.001)。在7 mm瞳孔直径下,3组组间术后SR、AR差异均有统计学意义(H=4.84,P=0.010;F=5.08,P=0.008);在4 mm瞳孔直径下,组间以上指标差异均无统计学意义(均P>0.05)。在4 mm瞳孔直径下,术后的视觉质量参数与术前暗瞳直径均无相关性(均P>0.05);在7 mm瞳孔直径下SR、AR与术前暗瞳直径呈负相关(r=-0.25,P=0.018;r=-0.32,P=0.002),HOAs、彗差、球差与术前暗瞳直径呈正相关(r=0.70、0.61、0.80,均P<0.001)。结论:ICL植入术后早期明、暗环境下视觉质量均较术前改善。暗视环境下,HOAs、彗差、球差、SR、AR与术前暗瞳直径有关,三叶草像差与术前暗瞳直径无关且手术前后变化最小。

Long-term clinical observation of patients with acute and chronic complete spinal cord injury after transplantation of Neuro Regen scaffold

Spinal cord injury(SCI)often results in an inhibitory environment at the injury site.In our previous studies,transplantation of a scaffold combined with stem cells was proven to induce neural regeneration in animal models of complete SCI.Based on these preclinical studies,collagen scaffolds loaded with the patients’own bone marrow mononuclear cells or human umbilical cord mesenchymal stem cells were transplanted into SCI patients.Fifteen patients with acute complete SCI and 51 patients with chronic complete SCI were enrolled and followed up for 2 to 5 years.No serious adverse events related to functional scaffold transplantation were observed.Among the patients with acute SCI,five patients achieved expansion of their sensory positions and six patients recovered sensation in the bowel or bladder.Additionally,four patients regained voluntary walking ability accompanied by reconnection of neural signal transduction.Among patients with chronic SCI,16 patients achieved expansion of their sensation level and 30 patients experienced enhanced reflexive defecation sensation or increased skin sweating below the injury site.Nearly half of the patients with chronic cervical SCI developed enhanced finger activity.These long-term follow-up results suggest that functional scaffold transplantation may represent a feasible treatment for patients with complete SCI.

A nanotheranostic agent based on Nd^(3+)-doped YVO_(4) with blood-brain-barrier permeability for NIR-Ⅱ fluorescence imaging/magnetic resonance imaging and boosted sonodynamic therapy of orthotopic glioma

The specific diagnosis and treatment of gliomas is a primary challenge in clinic due to their high invasiveness and blood-brain barrier(BBB)obstruction.It is highly desirable to find a multifunctional agent with good BBB penetration for precise theranostics.Herein,we design and construct a core-shell structured nanotheranostic agent(YVO_(4):Nd^(3+)-HMME@MnO_(2)-LF,marked as YHM)with YVO_(4)∶Nd^(3+) particles as the core and MnO_(2) nanosheets as the shell.Sonosensitizer hematoporphyrinmonomethyl ether(HMME)and lactoferrin(LF)were further loaded and modified on the surface,giving it a good ability to cross the BBB,near-infrared fluorescence imaging in the second window(NIR-Ⅱ)/magnetic resonance imaging(MRI)bimodality,and highly efficient sonodynamic therapy(SDT)of orthotopic gliomas.The YVO_(4):Nd^(3+)(25%)core exhibited good NIR-Ⅱ fluorescence properties,enabling YHM to act as promising probes for NIR-Ⅱ fluorescence imaging of vessels and orthotopic gliomas.MnO_(2) shell can not only provide O2 in the tumor microenvironments(TME)to significantly improve the healing efficacy of SDT,but also release Mn^(2+) ions to achieve T1-weight MRI in situ.Non-invasive SDT can effectively restrain tumor growth.This work not only demonstrates that multifunctional YHM is promising for diagnosis and treatment of orthotopic glioma,but also provides insights into exploring the theranostic agents based on rare earth-doped yttrium vanadate nanoparticles.

Two-dimensional square transition metal dichalcogenides with lateral heterostructures

Fabrication of lateral heterostructures （LHS） is promising for a wide range of next-generation devices and could sufficiently unlock the potential of two-dimensional materials.Herein,we demonstrate the design of lateral heterostructures based on new building materials,namely 1S-MX2 LHS,using first-principles calculations.1S-MX2 LHS exhibits excellent stability,demonstrating high feasibility in the experiment.The desired bandgap opening can endure application at room temperature and was confirmed in 1S-MX2 LHS with spin-orbit coupling （SOC）.A strain strategy further resulted in efficient bandgap engineering and an intriguing phase transition.We also found that black phosphorus can serve as a competent substrate to support 1S-MX2 LHS with a coveted type-Ⅱ band alignment,allowing versatile functionalized bidirectional heterostructures with built-in device functions.Furthermore,the robust electronic features could be maintained in the 1S-MX2 LHS with larger components.Our findings will not only renew interest in LHS studies by enriching their categories and properties,but also highlight the promise of these lateral heterostructures as appealing materials for future integrated devices.

Novel multifunctional theranostic nanoagents based on Ho^(3+) for CT/MRI dual-modality imaging-guided photothermal therapy

Because of the intrinsic defects of traditional treatment of cancer,it is quite needed to construct novel theranostic nanoagents that can not only improve the accuracy of imaging diagnosis but also achieve highly efficient therapy of cancer.Herein,we fabricated polydopamine-functionalized ammonium holmium fluoride nanocomposites(AHF@PDA)for dual-modality bioimaging(magnetic resonance imaging(MRI)and computed tomography(CT))owing to the high X-ray attenuation feature and magnetic property of Ho^(3+).Moreover,PDA shell endows AHF@PDA with excellent photothermal conversion performances and robust biocompatibility,leading to good treatment effect in vitro and in vivo.All above positive results certify that AHF@PDA have good potential as theranostic agents for clinical application in the future.

Carrier-free programmed spherical nucleic acid for effective ischemic stroke therapy via self-delivery antisense oligonucleotide

Antisense oligonucleotide(ASO)for anti-apoptosis is emerging as a highly promising therapeutic agents for ischemic stroke with complex pathological environment.However,its therapeutic efficacy is seriously limited by a number of challenges including inefficient internalization,low blood-brain barrier(BBB)penetration,poor stability,and potential toxicity of the carrier.Herein,a carrier-free programmed spherical nucleic acid nanostructure is developed for effective ischemic stroke therapy via integrating multifunctional modules into one DNA structure.By co-encoding caspase-3-ASO and transferrin receptor(TfR)aptamer into circle template,the spherical nucleic acid nanostructure(TD)was obtained via self-assembly.The experimental results demonstrated that the developed TD displayed efficient BBB penetration capability(6.4 times)and satisfactory caspase-3 silence effect(2.3 times)due to the dense DNA packaging in TD.Taken together,our study demonstrated that the carrier-free programmed spherical nucleic acid nanostructure could significantly improve the therapeutic efficacy of ischemic stroke and was a promising therapeutic tool for various brain damage-related diseases.

JUNO sensitivity on proton decay p→νK^(+)searches

The Jiangmen Underground Neutrino Observatory(JUNO)is a large liquid scintillator detector designed to explore many topics in fundamental physics.In this study,the potential of searching for proton decay in the p→νK^(+)mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification.Moreover,the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals.Based on these advantages,the detection efficiency for the proton decay via p→νK^(+)is 36.9%±4.9%with a background level of 0.2±0.05(syst)±0.2(stat)events after 10 years of data collection.The estimated sensitivity based on 200 kton-years of exposure is 9.6×1033 years,which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies.

食用蔬菜能吸收和积累微塑料

微塑料(100 nm～5 mm)作为一种新型环境污染物,具有潜在的动植物和人体健康风险,其污染已成为高度关注的全球环境问题.当前已有不少关于微塑料在水生生物体内积累的报道,但对于陆地生态系统的研究则相对匮乏,高等植物对微塑料的吸收和积累更未见报道.本文基于室内培养实验报道了微塑料在生菜(Lactuca sativa)体内的吸收、传输及分布.通过激光扫描共聚焦荧光显微镜和扫描电子显微镜观察发现,聚苯乙烯微球(0.2μm)可被生菜根部大量吸收和富集,并从根部迁移到地上部,积累和分布在可被直接食用的茎叶之中.研究结果为开展土壤-植物系统中微塑料积累机制及食物链传递与健康风险研究提供了新依据.

Sub-percent precision measurement of neutrino oscillation parameters with JUNO

JUNO is a multi-purpose neutrino observatory under construction in the south of China.This publication presents new sensitivity estimates for the measurement of the △m_(31)^(2),△m_(21)^(2),sin^(2)θ_(12),and sin^(2)θ_(13) oscillation parameters using reactor antineutrinos,which is one of the primary physics goals of the experiment.The sensitivities are obtained using the best knowledge available to date on the location and overburden of the experimental site,the nuclear reactors in the surrounding area and beyond,the detector response uncertainties,and the reactor antineutrino spectral shape constraints expected from the TAO satellite detector.It is found that the △m_(21)^(2) and sin^(2)θ_(12) oscillation parameters will be determined to 0.5%precision or better in six years of data collection.In the same period,the △m_(31)^(2) parameter will be determined to about 0.2%precision for each mass ordering hypothesis.The new precision represents approximately an order of magnitude improvement over existing constraints for these three parameters.

Adenomyosis-derived extracellular vesicles endow endometrial epithelial cells with an invasive phenotype through epithelial-mesenchymal transition

Extracellular vesicles from highly metastatic tumor cells have been shown to mediate epithelial-mesenchymal transition(EMT)-related events in recipient cells.In endometrial epithelial cells,EMT processes are known to be involved in the development of adenomyosis.We aimed to investigate whether adenomyosis-derived extracellular vesicles(AMEVs)are able to induce an EMT process in endometrial epithelial cells.In this study,AMEVs were isolated from patients with adenomyosis and characterized by transmission electron microscopy,Western blot,and nanoparticle tracking.Primary endometrial epithelial cells(EECs)were derived from normal endometrium tissues from patients with leiomyoma and co-cultured with AMEVs in vitro.AMEV uptake was examined by fluorescence confocal microscopy.The invasion of EECs was confirmed by Transwell assay.Immunohistochemistry,Western blot,and qRT-PCR were performed on EECs to illustrate the expression levels of cytokeratin 19,E-cadherin,vimentin,and zinc finger E-boxbinding homeobox 1(ZEB1).The results indicated that the cellular fluorescence intensity gradually increased after 48 h of co-culture,but decreased after 72 h.After co-culturing with AMEVs for 72 h,EECs expressed significantly lower levels of cytokeratin 19 and E-cadherin,and significantly higher levels of vimentin and ZEB1.Together these results demonstrated that AMEVs induce an EMT process and enhance the invasion of EECs.These changes may contribute to the pathogenesis and progression of adenomyosis.

Improvement in Mechanical Properties and Biocompatibility ofBiosynthetic Bacterial Cellulose/Lotus Root Starch Composites

Bacterial cellulose/lotus root starch(BC/LRS) composites were prepared by cultivating Acetobacter xylinum in nutrient media containing gelatinized lotus root starch. Low concentrations of gelatinized LRS had increased BC production with the maximum value at 6.67 g/L when 5 g/L of LRS was added in the culture media and the composites had thicker and denser fibrils compared with those of BC with low concentrations of LRS(2.5 and 5 g/L). When the concentration of LRS was increased above 7.5 g/L, the morphology of the BC/LRS composites contained more fibril layers that were linked with LRS. The results from X-ray diffraction(XRD) demonstrated that there was no significant difference in structure between BC and BC/LRS composites except a slight increase in crystallinity for BC/LRS composites as the concentration of LRS was lifted up. The tensile tests were performed to display BC/LRS composites prepared with LRS concentration at 2.5 and 5 g/L in media had the tensile strength of 54 and 60 MPa, respectively, which indicated an improvement in mechanical property compared to the unmodified BC(45 MPa). Live/dead assay with chondrocytes seeded on BC/LRS composite revealed higher cell viability ranging from 85% to 90% than BC. Furthermore, cell morphology with typical spindle shape was observed on the surfaces of BC/LRS composite by confocal microscope. Through the overall results, it shows that this study has provided a guidance to prepare BC/LRS composites with better cell biocompatibility and higher mechanical strength than those of BC for the potential use in cartilage tissue engineering.