We compared efficiencies of different PMD compensation feedback methods against transmission signal bandwidth, including NRZ, RZ, CRZ format under various duty cycles. We found that the critical factor determining the...We compared efficiencies of different PMD compensation feedback methods against transmission signal bandwidth, including NRZ, RZ, CRZ format under various duty cycles. We found that the critical factor determining the efficiency of PMD compensation is not the modulation format, but the spectral bandwidth of the transmission signal.展开更多
The combination of disease-specific human induced pluripotent stem cells(iPSC)and directed cell differentiation offers an ideal platform for modeling and studying many inherited human diseases.Wilson’s disease(WD)is ...The combination of disease-specific human induced pluripotent stem cells(iPSC)and directed cell differentiation offers an ideal platform for modeling and studying many inherited human diseases.Wilson’s disease(WD)is a monogenic disorder of toxic copper accumulation caused by pathologic mutations of the ATP7B gene.WD affects multiple organs with primary manifestations in the liver and central nervous system(CNS).In order to better investigate the cellular pathogenesis of WD and to develop novel therapies against various WD syndromes,we sought to establish a comprehensive platform to differentiate WD patient iPSC into both hepatic and neural lineages.Here we report the generation of patient iPSC bearing a Caucasian population hotspot mutation of ATP7B.Combining with directed cell differentiation strategies,we successfully differentiated WD iPSC into hepatocyte-like cells,neural stem cells and neurons.Gene expression analysis and cDNA sequencing confirmed the expression of the mutant ATP7B gene in all differentiated cells.Hence we established a platform for studying both hepatic and neural abnormalities of WD,which may provide a new tool for tissue-specific disease modeling and drug screening in the future.展开更多
文摘We compared efficiencies of different PMD compensation feedback methods against transmission signal bandwidth, including NRZ, RZ, CRZ format under various duty cycles. We found that the critical factor determining the efficiency of PMD compensation is not the modulation format, but the spectral bandwidth of the transmission signal.
基金supported by Sanofi-Aventis,The Helmsley Charitable Trust and The Ellison Medical Foundationsupported by"Thousand Young Talents"program of China+3 种基金National Laboratory of Biomacromolecules,Strategic Priority Research Program of the Chinese Academy of Sciencesa CIRM training grant fellowship(No.TG2-01158)a Glenn foundation grantpartially supported by an AFAR/Ellison Medical Foundation postdoctoral fellowship.
文摘The combination of disease-specific human induced pluripotent stem cells(iPSC)and directed cell differentiation offers an ideal platform for modeling and studying many inherited human diseases.Wilson’s disease(WD)is a monogenic disorder of toxic copper accumulation caused by pathologic mutations of the ATP7B gene.WD affects multiple organs with primary manifestations in the liver and central nervous system(CNS).In order to better investigate the cellular pathogenesis of WD and to develop novel therapies against various WD syndromes,we sought to establish a comprehensive platform to differentiate WD patient iPSC into both hepatic and neural lineages.Here we report the generation of patient iPSC bearing a Caucasian population hotspot mutation of ATP7B.Combining with directed cell differentiation strategies,we successfully differentiated WD iPSC into hepatocyte-like cells,neural stem cells and neurons.Gene expression analysis and cDNA sequencing confirmed the expression of the mutant ATP7B gene in all differentiated cells.Hence we established a platform for studying both hepatic and neural abnormalities of WD,which may provide a new tool for tissue-specific disease modeling and drug screening in the future.
