Impact of alcoholic extract from sea cucumber(Holothuria parva)on letrozole-induced polycystic ovary syndrome in adult female rats

Objective:To explore the potential therapeutic impact of an alcoholic extract derived from the sea cucumber(Holothuria parva)on letrozole-induced polycystic ovary syndrome(PCOS)in adult female Sprague-Dawley rats.Methods:Sixteen female rats,aged 8 to 10 weeks,with a mean weight of(200±20)g,were randomly assigned to four groups:the control,PCOS,and two treatment groups receiving sea cucumber extract and metformin,respectively.PCOS was induced by administering letrozole at a dose of 1 mg/kg to initiate the treatment period at 60 days of age.The study spanned four weeks,during which ovarian and uterine tissues were collected for histological examination,and blood samples were collected for hormonal levels.Results:Significant decreases in luteinizing hormone/follicle stimulating hormone(LH/FSH)and testosterone and increases progesterone levels among groups treated with sea cucumber extract were observed.While no significant differences were observed in follicle-stimulating hormone(FSH)and testosterone levels,the distinctive variations in key hormones suggested a potential role for sea cucumber alcoholic extract in ameliorating PCOS symptoms.Conclusions:This research highlights the moderate efficacy of sea cucumber extract as a therapeutic intervention for PCOS,providing a novel perspective in the search for effective remedies.The observed hormonal alterations,particularly in LH,estradiol,and progesterone,underscore the need for further exploration into the underlying mechanisms and for optimizing the application of sea cucumber extract in PCOS management.

A comparison of the effects of honey,fish oil and their combination on wound healing in rat

Objective:To evaluate and compare the effects of honey and fish oil on wound healing in rat.Methods:A total of 80 adult female Sprague-Dawley rats were randomly divided into four groups as negative control group,Groups II,III and IV.The rats in the four groups were treated with honey,fish oil and honey+fish oil,respectively.Rats were anesthetized,hair was removed from the back,then a wound was made on the back.Visual observation,histopathological examination and biomechanical study were performed on days 3,7,14 and 21 after operation.The data were analyzed using One-way ANOVA.Results:Wound area in group IV was lower than that in other groups.Promotion of wound contraction and epithelialization in group IV was better than that in other groups.Biomechanical parameters in group IV was significantly more than other rats.Conclusions:A combination of honey and fish oil on wounds can enhance healing better than honey and fish oil separately.