Directly monitoring mitophagy-specific viscosity dynamic in living cells is of great significance but remains challenging.Herein,this study reported a novel mitochondria-targeted fluorescent probe DPACDY based on vibr...Directly monitoring mitophagy-specific viscosity dynamic in living cells is of great significance but remains challenging.Herein,this study reported a novel mitochondria-targeted fluorescent probe DPACDY based on vibration-induced emission(VIE)for monitoring viscosity changes during mitochondrial autophagy.This probe contained N,N’-diphenyl-dihydrodibenzo[a,c]phenazine(DPAC)as the VIE core and two positively charged pyridinium moieties for mitochondria anchoring.As the ambient viscosity increased,the vibration of DPAC-DY could be hindered,and subsequently resulting in the enhancement of fluorescence emission.In vitro and intracellular experiments indicated that the probe DPAC-DY showed highly sensitive response to viscosity due to VIE mechanism.Importantly,by virtue of this probe,in situ and real-time visualization of the specific viscosity dynamics during the mitochondrial autophagy process was achieved.Thus,this work provides a novel strategy for VIE-based viscosity response sensors applied to specific organelles and offers a platform for in-depth study of mitochondrial viscosity-related diseases.展开更多
Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient ser...Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases.展开更多
Well-developed mitochondria-targeted nanocarriers for function regulation are highly desirable.Numerous studies have been conducted on the treatment of mitochondria-related diseases;however,further improvements are re...Well-developed mitochondria-targeted nanocarriers for function regulation are highly desirable.Numerous studies have been conducted on the treatment of mitochondria-related diseases;however,further improvements are required to develop more effective drug delivery methods.Herein,we comprehensively introduce recent developments progress in rational design of mitochondria-targeted nanocarriers,and discuss the different strategies of available nanocarriers for targeting mitochondria.We also highlight the advantages and disadvantages of various carrier systems that are currently in use.Finally,perspective on new generation for mitochondria-targeted delivery systems in the emerging area of drug-based therapeutics is also discussed.展开更多
基金the National Natural Science Foundation of China(Nos.81672508 and 61601218)Key University Science Research Project of Jiangsu Province(No.19KJA520005)+3 种基金Jiangsu Provincial Foundation for Distinguished Young Scholars(No.BK20170041)Natural Science Basic Research Program of Shaanxi(No.2019JM-016)China-Sweden Joint Mobility Project(No.51811530018)Fundamental Research Funds for the Central Universities。
文摘Directly monitoring mitophagy-specific viscosity dynamic in living cells is of great significance but remains challenging.Herein,this study reported a novel mitochondria-targeted fluorescent probe DPACDY based on vibration-induced emission(VIE)for monitoring viscosity changes during mitochondrial autophagy.This probe contained N,N’-diphenyl-dihydrodibenzo[a,c]phenazine(DPAC)as the VIE core and two positively charged pyridinium moieties for mitochondria anchoring.As the ambient viscosity increased,the vibration of DPAC-DY could be hindered,and subsequently resulting in the enhancement of fluorescence emission.In vitro and intracellular experiments indicated that the probe DPAC-DY showed highly sensitive response to viscosity due to VIE mechanism.Importantly,by virtue of this probe,in situ and real-time visualization of the specific viscosity dynamics during the mitochondrial autophagy process was achieved.Thus,this work provides a novel strategy for VIE-based viscosity response sensors applied to specific organelles and offers a platform for in-depth study of mitochondrial viscosity-related diseases.
基金supported by the National Key Research and Development Project (No.2020YFA0709900)the National Natural Science Foundation of China (Nos.81871265,81672508,81702617)+4 种基金Key University Science Research Project of Jiangsu Province (No.19KJA520005)Natural Science Basic Research Program of Shaanxi Province (No.2019JM-016)National Natural Science Foundation of Jiangxi Province (No.20202BABL206043)Science and Technology Project of Jiangxi Provincial Health Commission (No.20204495)Open Research Fund of Anhui Key Laboratory of Tobacco。
文摘Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases.
基金financial support from the National Key Research and Development Project (No. 2020YFA0709900)the Key University Science Research Project of Jiangsu Province (No. 19KJA520005)+1 种基金the National Natural Science Foundation of China (No. 22077101)the Open Project Program of State Key Laboratory of Coordination Chemistry (No. 202008)
文摘Well-developed mitochondria-targeted nanocarriers for function regulation are highly desirable.Numerous studies have been conducted on the treatment of mitochondria-related diseases;however,further improvements are required to develop more effective drug delivery methods.Herein,we comprehensively introduce recent developments progress in rational design of mitochondria-targeted nanocarriers,and discuss the different strategies of available nanocarriers for targeting mitochondria.We also highlight the advantages and disadvantages of various carrier systems that are currently in use.Finally,perspective on new generation for mitochondria-targeted delivery systems in the emerging area of drug-based therapeutics is also discussed.