Objective The aim of the study was to investigate whether ethanolic extract of propolis inhibits the growth and induces apoptosis of HL-60 cells. Methods HL-60 cells were treated for 24, 48, 72 h with various concentr...Objective The aim of the study was to investigate whether ethanolic extract of propolis inhibits the growth and induces apoptosis of HL-60 cells. Methods HL-60 cells were treated for 24, 48, 72 h with various concentrations ethanolic extracts of propolis(0, 50, 100, and 200 μg/m L). The proliferation of HL-60 cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. Subsequently, Hochest 33258 staining and terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) were used to test the apoptosis of HL-60 cells. We observed the expression levels of Bax and Bcl-2 in HL-60 cells by immunohistochemistry. Results MTT assay showed that various concentrations of ethanolic extract of propolis had significant inhibitory effect on HL-60 cell proliferation(P < 0.05). Typical morphologic changes could be observed by fluorescence microscope and TUNEL. By immunohistochemistry, we found the expression level of Bax was up-regulated, whereas that of Bc1-2 was down-regulated(P < 0.05). Conclusion Ethanolic extract of propolis inhibits leukemia cell proliferation and induces apoptosis in vitro. Its mechanism may be related to the regulation of Bax and Bcl-2 expression and up-regulation of Bcl-2/Bax ratio.展开更多
基金Supported by grants from the Shandong Province Natural Science Fund Project(No.ZR2013HL004)Colleges and Universities in Shandong Province Science and Technology Plan(No.J10LF57)Binzhou Medical College Students of Science and Technology Innovation Fund Project(No.BY2014DKCX003)
文摘Objective The aim of the study was to investigate whether ethanolic extract of propolis inhibits the growth and induces apoptosis of HL-60 cells. Methods HL-60 cells were treated for 24, 48, 72 h with various concentrations ethanolic extracts of propolis(0, 50, 100, and 200 μg/m L). The proliferation of HL-60 cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. Subsequently, Hochest 33258 staining and terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) were used to test the apoptosis of HL-60 cells. We observed the expression levels of Bax and Bcl-2 in HL-60 cells by immunohistochemistry. Results MTT assay showed that various concentrations of ethanolic extract of propolis had significant inhibitory effect on HL-60 cell proliferation(P < 0.05). Typical morphologic changes could be observed by fluorescence microscope and TUNEL. By immunohistochemistry, we found the expression level of Bax was up-regulated, whereas that of Bc1-2 was down-regulated(P < 0.05). Conclusion Ethanolic extract of propolis inhibits leukemia cell proliferation and induces apoptosis in vitro. Its mechanism may be related to the regulation of Bax and Bcl-2 expression and up-regulation of Bcl-2/Bax ratio.
