Lots of plant transcription factors have been identified in the recent decade, notably the knotted-1 related transcription factor family, the MADS-box transcription factors family, and factors involved in induction of...Lots of plant transcription factors have been identified in the recent decade, notably the knotted-1 related transcription factor family, the MADS-box transcription factors family, and factors involved in induction of floral meristem and those controlling cellular differentiation in plants. These transcription factors not only play an important role in the growth, development,展开更多
The intron is an important component of eu-karyotic gene. Extensive studies have been conducted to get a better understanding of its structure and function. This paper presents a brief review of the structure and func...The intron is an important component of eu-karyotic gene. Extensive studies have been conducted to get a better understanding of its structure and function. This paper presents a brief review of the structure and function of introns in higher plant genes. It is shown that higher plant introns possess structural properties shared by all eukaryotic introns, however, they also exhibit a striking degree of diversity. The process of intron splicing in higher plant genes involves interaction between multiple cis -acting elements and trans-acting factors, such as 5’ splicing site, 3’ splicing site and many protein factors. The process of intron splicing is an important level at which gene expression is regulated. Especially alternative splicing of intron can regulate time and space of gene expression. In addition, some introns in higher plant genes also regulate gene expression by affecting the pattern of gene expression, enhancing the level of gene expression and driving the gene expression.展开更多
mRNA differential display was used in analyzing the expression of carrot somatic embryo radicle gene in different developmental stages brought about by controlled cultivation. As shown by experiment, marked difference...mRNA differential display was used in analyzing the expression of carrot somatic embryo radicle gene in different developmental stages brought about by controlled cultivation. As shown by experiment, marked differences in electrophoresis patterns of DNA exist between the normally developing carrot radicle and those with their development inhibited. 12 series of specific bands have been cloned, and structural analysis of them is under way.展开更多
The photoperiod_sensitive genic male sterile rice (PGMR) is particularly useful to take advantage of heterosis in rice. mRNA differential display was used to isolate the fertility_relative genes in rice. After establi...The photoperiod_sensitive genic male sterile rice (PGMR) is particularly useful to take advantage of heterosis in rice. mRNA differential display was used to isolate the fertility_relative genes in rice. After establishing an optimized mRNA differential display system, one of the differential cDNA fragments that maybe related to the development and maturation of rice panicle was cloned from a PGMR Nongken 58S.展开更多
文摘Lots of plant transcription factors have been identified in the recent decade, notably the knotted-1 related transcription factor family, the MADS-box transcription factors family, and factors involved in induction of floral meristem and those controlling cellular differentiation in plants. These transcription factors not only play an important role in the growth, development,
文摘The intron is an important component of eu-karyotic gene. Extensive studies have been conducted to get a better understanding of its structure and function. This paper presents a brief review of the structure and function of introns in higher plant genes. It is shown that higher plant introns possess structural properties shared by all eukaryotic introns, however, they also exhibit a striking degree of diversity. The process of intron splicing in higher plant genes involves interaction between multiple cis -acting elements and trans-acting factors, such as 5’ splicing site, 3’ splicing site and many protein factors. The process of intron splicing is an important level at which gene expression is regulated. Especially alternative splicing of intron can regulate time and space of gene expression. In addition, some introns in higher plant genes also regulate gene expression by affecting the pattern of gene expression, enhancing the level of gene expression and driving the gene expression.
文摘mRNA differential display was used in analyzing the expression of carrot somatic embryo radicle gene in different developmental stages brought about by controlled cultivation. As shown by experiment, marked differences in electrophoresis patterns of DNA exist between the normally developing carrot radicle and those with their development inhibited. 12 series of specific bands have been cloned, and structural analysis of them is under way.
文摘The photoperiod_sensitive genic male sterile rice (PGMR) is particularly useful to take advantage of heterosis in rice. mRNA differential display was used to isolate the fertility_relative genes in rice. After establishing an optimized mRNA differential display system, one of the differential cDNA fragments that maybe related to the development and maturation of rice panicle was cloned from a PGMR Nongken 58S.
