Parasitic contamination of surface and deep soil in different areas of Sari in north of Iran

Objective:To study the parasitic contamination of soil in selected areas of Sari,north of Iran.Methods:A cross-sectional study was conducted to identify all available parasites in surface and deep soil.In this study 580 soil samples(278 deep soil and 302 topsoil samples)from 21 different locations were collected from pathways,parks,greenhouses,estates around the city,cemetery,main squares,farmlands,fenced gardens and seashores.Depending on the soil type,two samples were prepared,from surface and deep soil at the depth of 3 to 5 cm.After performing various stages of preparation,including cleaning and washing,smoothing and flotation,parasitic elements were examined microscopically and quantitative parasite counting was done using a McMaster slide.Results:The results showed that the highest rate of parasitic contamination was related to nematodes larvae(26.11%).Other contaminants such as Entamoeba and Acanthamoeba cysts,vacuolization Blastocystis hominis form,oocyte containing sporocysts,Toxascaris eggs,nematoda larvae,Hymenolepis eggs,Ascaris eggs,Fasciola eggs,hookworm eggs,Toxocara eggs,insects'larvae and other ciliated and flagellated organisms were also observed.The results of this study showed that the highest contamination was found in public garden(25.80%)both in surface(29.30%)and in deep soil(21.12%),while the lowest level of contamination was observed in seashore surface soil(4.90%).Conclusions:The results showed that soil can provide a potential medium for the spread of soil transmitted parasitic diseases in the environment;therefore,preventive programs are needed.