Since ancient times,humans have bred several plants that we rely on today.However,little is known about the divergence of most of these plants.In the present study,we investigated the divergence of Mibuna(Brassica rap...Since ancient times,humans have bred several plants that we rely on today.However,little is known about the divergence of most of these plants.In the present study,we investigated the divergence of Mibuna(Brassica rapa L.subsp.nipposinica L.H.Bailey),a traditional leafy vegetable in Kyoto(Japan),by combining genetic analysis and a survey of ancient literature.Mibuna is considered to have been bred 200 years ago from Mizuna,another traditional leafy vegetable in Kyoto.Mibuna has simple spatulate leaves,whereas Mizuna has characteristic serrated leaves.The quantitative trait loci(QTL)and gene expression analyses suggested that the downregulation of BrTCP15 expression contributed to the change in the leaf shape from serrated to simple spatulate.Interestingly,the SNP analysis indicated that the genomic region containing the BrTCP15 locus was transferred to Mibuna by introgression.Furthermore,we conducted a survey of ancient literature to reveal the divergence of Mibuna and found that hybridization between Mizuna and a simple-leaved turnip might have occurred in the past.Indeed,the genomic analysis of multiple turnip cultivars showed that one of the cultivars,Murasakihime,has almost the same sequence in the BrTCP15 region as Mibuna.These results suggest that the hybridization between Mizuna and turnip has resulted in the establishment of Mibuna.展开更多
基金This research was supported by grants in aid from the Japan Society for the Promotion of Science(KAKENHI grant numbers 16H01472 and 18H04787)the MEXT-Supported Program for the Strategic Research Foundation at Private Universities(grant number S1511023)from the Ministry of Education,Culture,Sports,Science,and Technology of Japan to S.K+2 种基金This work was also financially supported by the Sasakawa Scientific Research Grant from The Japan Science Society to Y.KThis study was supported by a Joint Usage/Research program of the Center for Ecological Research,Kyoto University,the Ministry of Education,Culture,Sports,Science,and Technology of Japan(MEXT)[Grant-in-Aid for Scientific Research on Innovative Areas(19H04870)]Research Funding for the Computational Software Supporting Program from Meiji University.
文摘Since ancient times,humans have bred several plants that we rely on today.However,little is known about the divergence of most of these plants.In the present study,we investigated the divergence of Mibuna(Brassica rapa L.subsp.nipposinica L.H.Bailey),a traditional leafy vegetable in Kyoto(Japan),by combining genetic analysis and a survey of ancient literature.Mibuna is considered to have been bred 200 years ago from Mizuna,another traditional leafy vegetable in Kyoto.Mibuna has simple spatulate leaves,whereas Mizuna has characteristic serrated leaves.The quantitative trait loci(QTL)and gene expression analyses suggested that the downregulation of BrTCP15 expression contributed to the change in the leaf shape from serrated to simple spatulate.Interestingly,the SNP analysis indicated that the genomic region containing the BrTCP15 locus was transferred to Mibuna by introgression.Furthermore,we conducted a survey of ancient literature to reveal the divergence of Mibuna and found that hybridization between Mizuna and a simple-leaved turnip might have occurred in the past.Indeed,the genomic analysis of multiple turnip cultivars showed that one of the cultivars,Murasakihime,has almost the same sequence in the BrTCP15 region as Mibuna.These results suggest that the hybridization between Mizuna and turnip has resulted in the establishment of Mibuna.
