Bioinformatics comprehensive analysis confirmed the potential involvement of SLC22A1 in lower-grade glioma progression and prognosis

Background:Although it has been established that the human Solute Carrier Family 22(SLC22)functions as a cationic transporter,influencing cellular biological metabolism by modulating the uptake of various cations,its impact on cancer prognosis remains unclear.Methods:We conducted a comprehensive analysis utilizing data from The Cancer Genome Atlas(TCGA)and other databases to assess the prognostic value and functional implications across various tumors.Silence of SLC22A1 RNA in glioma U251 cells was performed to access the impact of SLC22A1 on lowergrade glioma(LGG)progression.Results:Our findings demonstrated a significant correlation between SLC22A1 expression and the survival time of patients with various cancers(p<0.05).Importantly,we found the potential involvement of SLC22A1 in occurrence and progress of certain cancers,with a pronounced impact on LGG.Further examination of the SLC22A1-LGG relationship revealed its status as an independent risk factor for LGG,suggesting its potential involvement in regulating diverse immune pathways and metabolic activities.Chinese Glioma Genome Atlas(CGGA)data supported the reliability of the risk score as a prognostic and recurrence indicator,emphasizing the accuracy of the nomograph(1,3,and 5-year-AUC>0.8).Cell proliferation and clone formation experiment proved that decreased expression of SLC22A1 in glioma U251 cells inhibited glioma cell growth.Conclusion:Our findings suggest SLC22A1 has huge prospects as a promising biomarker and therapeutic target for LGG prognosis.SLC22A1 has only been proved to support cellular function previously.Our findings demonstrated a robust connection between the tumor microenvironment and functional proteins that maintain basal cell metabolism,which gifts unique tumor immune characteristics of gliomas.Additionally,we provide a highly practical prediction model for estimating the survival rate of LGG patients.

Surface wettability and flow properties of non-metallic pipes in laminar flow

In this paper,three liquids flowing in five pipes with the same inner diameter of 14 mm were studied to determine the relationship between the surface wettability and flow properties in laminar flow(Re b 2000).This was motivated by oilfield observations of increased pressure drops in non-metallic pipes compared to those in metal pipes,which was contrary to expectations.A new expression for the frictional coefficient that considers the Reynolds number and contact angleθin laminar flow for non-metallic pipes was proposed based on the experimental results of single-phase flow using dimension and regression analyses.The solutions of the anomalous phenomenon were proposed from the perspectives of the pipe diameter,contact-angle difference,and the compatibility between flexible composite pipe and JLHW105 oil according to the new formula.The surprising finding was that the surface wettability could control the frictional resistance by the critical contact angle(39.9°)obtained at the same Reynolds number.If 0°bθ≤39.9°,the frictional coefficient increased as the contact angle increased.In contrast,if 39.9°bθb 180°,the frictional coefficient decreased with increasing contact angle.The influences of the pipe diameter and contactangle difference on the pressure drop difference of JLHW105 oil showed an inversely proportional relation.A series of materials and liquids were tested.The selection of pipe material for transporting a given fluid can be based on the contact angle,surface tension,and critical limit of the contact angle obtained.The research results are expected to provide some guidelines for the selection of the appropriate pipe material for a given set of fluids.

Beckmann重排合成N-(2-萘基)乙酰胺的综合实验设计

贝克曼重排反应实验是大学有机化学基础实验教学中的重要内容之一。目前实验教学中主要采用的方案是在浓硫酸或多聚磷酸的条件下回流反应。本实验通过三苯基膦和四氯化碳原位形成Appel盐引发反应,使贝克曼重排反应能够在中性、常温条件下进行。该实验方案避免了强腐蚀性酸和高温回流条件的使用,不仅具有操作简单、产物收率高、重复性好的特点,同时在实验安全方面表现出明显的优势。

四苯乙烯及其衍生物超声辅助合成与光电性能研究

四苯乙烯及其衍生物被广泛报道具有典型的聚集诱导发光(AIE)性能,在有机发光二极管、荧光离子探针、生物化学成像等诸多领域有着广阔的应用前景。以此为背景,可以将相关前沿议题转化为本科综合实验教学方案。本文针对现有的实验教学方案设计进行了探究和改进,在原有使用McMurry偶联反应制备四苯乙烯的实验方案基础上,利用生色团上不同取代基的推拉电子效应设计平行对照试验,合成溴代、甲氧基取代的四苯乙烯衍生物,实现了荧光发射光谱最大峰波长的显著移动,使得在紫外灯下即可观察到荧光从蓝色到黄绿色的变化。利用超声辅助合成的方法,成功将反应时间从原有的6–8 h压缩至2 h,使之更适应本科教学实验所需的时间空间。除光学性能表征外,本实验还综合了循环伏安法表征产物的能带结构。本实验针对原有方案进行了诸多改进,引入平行实验锻炼科学思维、别出心裁地引入超声辅助合成法,极大地缩短了反应时间,展现出较强的前沿性、绿色性和高效性。

Porous Pyrene Organic Cage with Unusual Absorption Bathochromic-Shift Enables Visible Light Photocatalysis

We have constructed a novel porous pyrene-based organic cage,PyTC1,through the condensation reaction of cyclohexanediamine with 5,5′-(pyrene-1,6-diyl)diisophthalaldehyde.Single-crystal X-ray diffraction analysis reveals the effective intercage C–H...πinteraction between cyclohexanediimine and pyrene segments.Such a soft intercage C–H...πinteraction,rather than a classic J-aggregate with slippedπ–π-stacking configuration,induced an unusual bathochromic shift of pyrene-based chromophore absorption from an ultraviolet region of PyTC1 in solution to the visible light region of PyTC1 in solid-state.

Synthesis of high-purity Ti2SC powder by microwave hybrid heating

A novel simple method is presented to synthesize high-purity Ti2SC powder using Ti/C/S and Ti/C/TiS2 systems by microwave hybrid heating at different temperatures in argon atmosphere.It was confirmed that the synthesis temperature is strongly dependent on the starting composition.For Ti/C/S system,Ti2SC with small amounts of TiS and TiC was synthesized at 1200 ℃.For Ti/C/TiS2 system,high-purity Ti2SC was synthesized at 800 ℃ and above.The synthesis of Ti2SC powder at low temperature was attributed to the combination of microwave effect by microwave hybrid heating and the introduction of TiS2 as sulfur source.Scanning electron microscopy (SEM) analysis indicated that the layered structure of Ti2SC particles is perfectly formed at 1100 ℃,and the crystal particle size approaches to homogeneity which is about 2-5 μm.It was presumed that the formation mechanism of Ti/C/TiS2 system is that TiS2 firstly reacts with Ti to form Ti-S intermetallics,then Ti-S intermetallics reacts with un-reacted Ti and graphite to produce Ti2SC.

Precise fabrication of ternary ordered covalent organic frameworks for photocatalysis

Despite the great success in the synthesis of binary covalent organic frameworks(COFs)in the past decade,the fabrication of structurally ordered ternary COFs remains a big challenge,due to the inevitable competition and exchange of monomers.Herein,two ternary ordered COFs(2Me-OMe-COF and Me-2OMe-COF)are successfully synthesized by two-step polymerization based on irreversible covalent bonds.In contrast to the ternary random COFs synthesized by one-pot method,ternary ordered COFs possess definite structures at the molecular level.Accordingly,we systematically compare the performance of the two ternary ordered COFs in photocatalytic H_(2)production with two corresponding binary COFs as controlled comparison.Significantly,they exhibit very different photocatalytic performance,highlighting the importance of component and structural order for their properties.This work provides a unique strategy to the targeted polymerization and synthesis of ternary ordered,or even multivariate ordered COFs.