Impact of Land-Use Change on Soil Microbial Community Composition and Organic Carbon Content in the Dry Tropics

Restoration of forests poses a major challenge globally, particularly in the tropics, as the forests in these regions are more vulnerable to land-use change. We studied land-use change from natural forest(NF) to degraded forest(DF), and subsequently to either Jatropha curcas plantation(JP) or agroecosystem(AG), in the dry tropics of Uttar Pradesh, India, with respect to its impacts on soil microbial community composition as indicated by phospholipid fatty acid(PLFA) biomarkers and soil organic carbon(SOC) content. The trend of bacterial PLFAs across all land-use types was in the order: NF > JP > DF> AG. In NF, there was dominance of gram-negative bacterial(G^-) PLFAs over the corresponding gram-positive bacterial(G^+) PLFAs. The levels of G^- PLFAs in AG and JP differed significantly from those in DF, whereas those of G^+ PLFAs were relatively similar in these three land-use types. Fungal PLFAs,however, followed a different trend: NF > JP > DF = AG. Total PLFAs, fungal/bacterial(F/B) PLFA ratio, and SOC content followed trends similar to that of bacterial PLFAs. Across all land-use types, there were strong positive relationships between SOC content and G-, bacterial, fungal, and total microbial PLFAs and F/B PLFA ratio. Compared with bacterial PLFAs, fungal PLFAs appeared to be more responsive to land-use change. The F/B PLFA ratio, fungal PLFAs, and bacterial PLFAs explained 91%, 94%,and 73% of the variability in SOC content, respectively. The higher F/B PLFA ratio in JP favored more soil C storage, leading to faster ecosystem recovery compared to either AG or DF. The F/B PLFA ratio could be used as an early indicator of ecosystem recovery in response to disturbance, particularly in relation to land-use change.

Microbial Biomass Dynamics in a Tropical Agroecosystem： Influence of Herbicide and Soil Amendments

The influences of herbicide alone and in combination with the soil amendments with contrasting resource qualities on dynamics of soil microbial biomass C （MBC）, N （MBN）, and P （MBP） were studied through two annual cycles in rice-wheat-summer fallow crop sequence in a tropical dryland agroecosystem. The experiment included application of herbicide （butachlor） alone or in combination with various soil amendments having equivalent amount of N in the forms of chemical fertilizer, wheat straw, Sesbania aculeata, and farm yard manure （FYM）. Soil microbial biomass showed distinct temporal variations in both crop cycles, decreased from vegetative to grain-forming stage, and then increased to maximum at crop maturity stage. Soil MBC was the highest in herbicide ＋ Sesbania aculeata treatment followed by herbicide ＋ FYM, herbicide ＋ wheat straw, herbicide ＋ chemical fertilizer, and herbicide alone treatments in decreasing order during the rice-growing period. During wheat-growing period and summer fallow, soil MBC attained maximum for herbicide ＋ wheat straw treatment whereas herbicide ＋ FYM, herbicide ＋ Sesbania, and herbicide ＋ chemical fertilizer treatments showed similar levels. The overall trend of soil MBN was similar to those of soil MBC and MBP except that soil MBN was higher in herbicide ＋ chemical fertilizer treatment over the herbicide ＋ wheat straw treatment during rice-growing period. In spite of the addition of equivalent amount of N through exogenous soil amendments in combination with the herbicide, soil microbial biomass responded differentially to the treatments. The resource quality of the amendments had more pronounced impact on the dynamics of soil microbial biomass, which may have implications for long-term sustainability of rainfed agroecosystems in dry tropics.