Optical microscopy has become an indispensable tool for visualizing sub-cellular structures andbiological processes.However,scattering in biological tissues is a major obstacle that preventshigh-resolution images from...Optical microscopy has become an indispensable tool for visualizing sub-cellular structures andbiological processes.However,scattering in biological tissues is a major obstacle that preventshigh-resolution images from being obtained from deep regions of tissue.We review commontechniques,such as multiphoton microscopy(MPM)and optical coherence microscopy(OCM),for diffraction limited imaging beyond an imaging depth of 0.5 mm.Novel implementations havebeen emerging in recent years giving higher imaging speed,deeper penetration,and better imagequality.Focal modulation microscopy(FMM)is a novel method that combines confocal spatialfltering with focal modulation to reject out-of-focus background.FMM has demonstrated animaging depth comparable to those of MPM and OCM,near-real-time image acquisition,and thecapability for multiple contrast mechanisms.展开更多
Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues.However,existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial r...Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues.However,existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial resolution.Here we re-port a novel design and implementation of a powerful laser speckle imaging platform to solve the two critical limitations.The core technique of our platform is a combination of line scan confocal microscopy with laser speckle autocorrelation imaging,which is termed Line Scan Laser Speckle Autocorrelation Imaging(LS-LSAI).The technical advantages of LS-LSAI include high spatial resolution(~4.4μm)for visualizing and quantifying blood flow in microvessels,as well as video-rate imaging speed for tracing dynamic flow.展开更多
文摘Optical microscopy has become an indispensable tool for visualizing sub-cellular structures andbiological processes.However,scattering in biological tissues is a major obstacle that preventshigh-resolution images from being obtained from deep regions of tissue.We review commontechniques,such as multiphoton microscopy(MPM)and optical coherence microscopy(OCM),for diffraction limited imaging beyond an imaging depth of 0.5 mm.Novel implementations havebeen emerging in recent years giving higher imaging speed,deeper penetration,and better imagequality.Focal modulation microscopy(FMM)is a novel method that combines confocal spatialfltering with focal modulation to reject out-of-focus background.FMM has demonstrated animaging depth comparable to those of MPM and OCM,near-real-time image acquisition,and thecapability for multiple contrast mechanisms.
基金supports from Ministry of Education-Singapore(MOE2019-T2-2-094,R-397-000-327-114).
文摘Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues.However,existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial resolution.Here we re-port a novel design and implementation of a powerful laser speckle imaging platform to solve the two critical limitations.The core technique of our platform is a combination of line scan confocal microscopy with laser speckle autocorrelation imaging,which is termed Line Scan Laser Speckle Autocorrelation Imaging(LS-LSAI).The technical advantages of LS-LSAI include high spatial resolution(~4.4μm)for visualizing and quantifying blood flow in microvessels,as well as video-rate imaging speed for tracing dynamic flow.
