Investigation on fungal populations and mycotoxins of mouldy rapeseed was conducted in this paper. A total of 578 rapeseed samples were collected from 11 Chinese provinces in 2015, and 141 samples were contaminted by ...Investigation on fungal populations and mycotoxins of mouldy rapeseed was conducted in this paper. A total of 578 rapeseed samples were collected from 11 Chinese provinces in 2015, and 141 samples were contaminted by mycete. Mouldy rapeseed samples with exceeding specified standard were found in all areas. The highest percentages of samples were from Hunan, Jiangxi and Hubei provinces with above standard rate of 31.91%, 24.54% and 20.45% respectively. Mycete in 29 severely mouldy samples were isolatedand determined by using dilution method. Eighty-three dominant isolates were found,among which fungal strain number ranged from 1 to 8. Aspergillus flavus, Penicillium steckii and Penicillium citrinum were dominant myco-populations identified by morphological and molecular biological methods. Rapeseed from the Southern provinces including Jiangxi,Hunan and Guangxi were contaminated by more isolates. Mycotoxins content were evaluated by LC–MS/MS. AFB1, BEA and FB1 were the top 3 prevalent mycotoxins. BEA and FB1 contaminations in rapeseed were relatively low. This investigation provid support for protecting public health and ensuring consumption security of rapeseed.展开更多
文摘Investigation on fungal populations and mycotoxins of mouldy rapeseed was conducted in this paper. A total of 578 rapeseed samples were collected from 11 Chinese provinces in 2015, and 141 samples were contaminted by mycete. Mouldy rapeseed samples with exceeding specified standard were found in all areas. The highest percentages of samples were from Hunan, Jiangxi and Hubei provinces with above standard rate of 31.91%, 24.54% and 20.45% respectively. Mycete in 29 severely mouldy samples were isolatedand determined by using dilution method. Eighty-three dominant isolates were found,among which fungal strain number ranged from 1 to 8. Aspergillus flavus, Penicillium steckii and Penicillium citrinum were dominant myco-populations identified by morphological and molecular biological methods. Rapeseed from the Southern provinces including Jiangxi,Hunan and Guangxi were contaminated by more isolates. Mycotoxins content were evaluated by LC–MS/MS. AFB1, BEA and FB1 were the top 3 prevalent mycotoxins. BEA and FB1 contaminations in rapeseed were relatively low. This investigation provid support for protecting public health and ensuring consumption security of rapeseed.
