The motion of a lure in water is investigated experimentally and numerically.The lure motion in water of apassing water tank is observed,and the periodic motion is found.From the Fourier analysis,it is found that the ...The motion of a lure in water is investigated experimentally and numerically.The lure motion in water of apassing water tank is observed,and the periodic motion is found.From the Fourier analysis,it is found that the frequency with the largest amplitude in the lateral direction depends on the lip width of the lure.To understand the lure dynamics,a numerical simulation of the flow field around the lure is performed.The shape is measured using an X-ray computer tomography and converted into a voxel model.From visualization,it is found that vortex sheds from its lip correspond to the vibration frequency of the lure.展开更多
Optical microscopy is an indispensable tool in biomedical sciences,but its reach in deep tissues is limited due to aberrations and scattering.This problem can be overcome by wavefront-shaping techniques,albeit at limi...Optical microscopy is an indispensable tool in biomedical sciences,but its reach in deep tissues is limited due to aberrations and scattering.This problem can be overcome by wavefront-shaping techniques,albeit at limited fields of view(FOVs).Inspired by astronomical imaging,conjugate wavefront shaping can lead to an increased field of view in microscopy,but this correction is limited to a set depth and cannot be dynamically adapted.Here,we present a conjugate wavefront-shaping scheme based on focus scanning holographic aberration probing(F-SHARP).We combine it with a compact implementation that can be readily adapted to a variety of commercial and home-built two-photon microscopes.We demonstrate the power of the method by imaging with high resolution over extended FOV(>80μm)deeper than 400μm inside a mouse brain through a thinned skull.展开更多
基金supported in part by Tokyo Denki University Science Promotion Fund(Q12K-04)
文摘The motion of a lure in water is investigated experimentally and numerically.The lure motion in water of apassing water tank is observed,and the periodic motion is found.From the Fourier analysis,it is found that the frequency with the largest amplitude in the lateral direction depends on the lip width of the lure.To understand the lure dynamics,a numerical simulation of the flow field around the lure is performed.The shape is measured using an X-ray computer tomography and converted into a voxel model.From visualization,it is found that vortex sheds from its lip correspond to the vibration frequency of the lure.
基金funding by the German Science Foundation DFG(projects 326649520 and 327654276/SFB 1315)the Human Frontiers Science Programme+1 种基金a Starting Grant by the European Research Council(ERC-2016-StG-714560)the Alfried Krupp Prize for Young University Teachers,awarded by the Alfried Krupp von Bohlen und Halbach-Stiftung.
文摘Optical microscopy is an indispensable tool in biomedical sciences,but its reach in deep tissues is limited due to aberrations and scattering.This problem can be overcome by wavefront-shaping techniques,albeit at limited fields of view(FOVs).Inspired by astronomical imaging,conjugate wavefront shaping can lead to an increased field of view in microscopy,but this correction is limited to a set depth and cannot be dynamically adapted.Here,we present a conjugate wavefront-shaping scheme based on focus scanning holographic aberration probing(F-SHARP).We combine it with a compact implementation that can be readily adapted to a variety of commercial and home-built two-photon microscopes.We demonstrate the power of the method by imaging with high resolution over extended FOV(>80μm)deeper than 400μm inside a mouse brain through a thinned skull.
