Objective： This study examined the antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava against methicillin-resistant Staphylococcus aureus （MRSA） and used a standardized purification pro...Objective： This study examined the antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava against methicillin-resistant Staphylococcus aureus （MRSA） and used a standardized purification protocol to determine the presence and abundance of bioactive compounds in the leaf extracts. Methods： In vitro antimicrobial activities of the ethanolic extracts of C sativa, T. orientalis and P. guajava were tested against MRSA. The presence of bioactive molecules in these three leaves was evaluated using biochemical assays and high-performance thin-layer chromatography （HPTLC）. Results： Resistance to methicillin, penicillin, oxacillin and cefoxitin was observed in each of the clinical and nonclinical MRSA isolates. However, they were still vulnerable to vancomydn. Used individually, the 50% extract of each plant leaf inhibited MRSA growth. A profound synergism was observed when C sativa was used in combination with T. orientalis （1：1 ） and when P. guajava was used in combination with T. orientalis （1：1 ）. This was shown by larger zones of inhibition. This synergism was probably due to the combined inhibitory effect of phenolics present in the leaf extracts （i.e., quercetin and gallic acid） and catechin, as detected by HPTLC. Conclusion： The leaf extracts of C sativa, T. orientalis and P. guajava had potential for the control of both hospital- and community-acquired MRSA. Moreover, the inhibitory effect was enhanced when extracts were used in combination.展开更多
基金Saaii Educational Foundation, Kanpur-209203,India,for their financial assistance
文摘Objective： This study examined the antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava against methicillin-resistant Staphylococcus aureus （MRSA） and used a standardized purification protocol to determine the presence and abundance of bioactive compounds in the leaf extracts. Methods： In vitro antimicrobial activities of the ethanolic extracts of C sativa, T. orientalis and P. guajava were tested against MRSA. The presence of bioactive molecules in these three leaves was evaluated using biochemical assays and high-performance thin-layer chromatography （HPTLC）. Results： Resistance to methicillin, penicillin, oxacillin and cefoxitin was observed in each of the clinical and nonclinical MRSA isolates. However, they were still vulnerable to vancomydn. Used individually, the 50% extract of each plant leaf inhibited MRSA growth. A profound synergism was observed when C sativa was used in combination with T. orientalis （1：1 ） and when P. guajava was used in combination with T. orientalis （1：1 ）. This was shown by larger zones of inhibition. This synergism was probably due to the combined inhibitory effect of phenolics present in the leaf extracts （i.e., quercetin and gallic acid） and catechin, as detected by HPTLC. Conclusion： The leaf extracts of C sativa, T. orientalis and P. guajava had potential for the control of both hospital- and community-acquired MRSA. Moreover, the inhibitory effect was enhanced when extracts were used in combination.
