Calcium-independent phospholipase A<sub>2</sub> (iPLA<sub>2</sub>) belongs to the group VI family of phospholipase superfamily (PLA<sub>2</sub>) that catalyses the hydrolysis of gly...Calcium-independent phospholipase A<sub>2</sub> (iPLA<sub>2</sub>) belongs to the group VI family of phospholipase superfamily (PLA<sub>2</sub>) that catalyses the hydrolysis of glycerophospholipids at the sn-2 ester bond, producing unesterified fatty acids and 2-lysophospholipids. Research interests on iPLA<sub>2</sub> have not been as significant as those on secretary PLA<sub>2</sub> and cytosolic PLA<sub>2</sub>. However, more efforts have been made recently on understanding the expression, regulation and biological function of iPLA<sub>2</sub>. iPLA<sub>2</sub> plays important roles in several biological processes, including signal transduction, phospholipid remodelling, eicosanoid formation, cell proliferation, cell differentiation and apoptosis. Modulation of iPLA<sub>2</sub> activity can have prominent effects on cellular metabolism, central nervous system and cardiovascular functions. Thus, dysregulation iPLA<sub>2</sub> can play a vital role in the pathogenesis of several diseases. The aim of this review is to provide the current understanding of the structure, function and regulation of group VI iPLA<sub>2</sub> and highlight its potential mechanisms of action in mediating several neurological disorders and cancer.展开更多
Autoantibodies （aAbs） are generated by the immune system in response to a number of stimuli, one of which is believed to be aberrant protein targeting to the plasma surface. In this study, the presence of human cyto...Autoantibodies （aAbs） are generated by the immune system in response to a number of stimuli, one of which is believed to be aberrant protein targeting to the plasma surface. In this study, the presence of human cytochrome P450 enzyme CYP4Z1 on the outer surface of the plasma membrane of MCF-7 breast cancer cells is demonstrated by immunofluorescence. Moreover, anti- CYP4Z1 aAbs could clearly be detected in breast cancer patient sera by both immunoprecipitation and western blot analysis, while weak or no signals were obtained when testing controls.展开更多
文摘Calcium-independent phospholipase A<sub>2</sub> (iPLA<sub>2</sub>) belongs to the group VI family of phospholipase superfamily (PLA<sub>2</sub>) that catalyses the hydrolysis of glycerophospholipids at the sn-2 ester bond, producing unesterified fatty acids and 2-lysophospholipids. Research interests on iPLA<sub>2</sub> have not been as significant as those on secretary PLA<sub>2</sub> and cytosolic PLA<sub>2</sub>. However, more efforts have been made recently on understanding the expression, regulation and biological function of iPLA<sub>2</sub>. iPLA<sub>2</sub> plays important roles in several biological processes, including signal transduction, phospholipid remodelling, eicosanoid formation, cell proliferation, cell differentiation and apoptosis. Modulation of iPLA<sub>2</sub> activity can have prominent effects on cellular metabolism, central nervous system and cardiovascular functions. Thus, dysregulation iPLA<sub>2</sub> can play a vital role in the pathogenesis of several diseases. The aim of this review is to provide the current understanding of the structure, function and regulation of group VI iPLA<sub>2</sub> and highlight its potential mechanisms of action in mediating several neurological disorders and cancer.
文摘Autoantibodies （aAbs） are generated by the immune system in response to a number of stimuli, one of which is believed to be aberrant protein targeting to the plasma surface. In this study, the presence of human cytochrome P450 enzyme CYP4Z1 on the outer surface of the plasma membrane of MCF-7 breast cancer cells is demonstrated by immunofluorescence. Moreover, anti- CYP4Z1 aAbs could clearly be detected in breast cancer patient sera by both immunoprecipitation and western blot analysis, while weak or no signals were obtained when testing controls.
