Isolation and Identification of Multi-Drug Resistant Strains of Non-Lactose Fermenting Bacteria from Clinical Refuses in Major Hospitals of Khyber Pakhtunkhwa, Pakistan

Purpose: The present studied was performed in order to investigate the drug resistance of different non-lactose fermenting gram negative bacteria from clinical isolates. The bacteria were morphologically characterized through culturing and gram staining techniques were used for the identification of different bacterial strains. Methods: A total of 324 samples were collected from patients, after they were diagnosed by physicians at different hospitals at district Peshawar. Samples were morphologically identified by blood agar, MacConkey agar and Eosine Methylene Blue, identified by gram staining techniques. Modified Kirby-Bauer Disc diffusion method was used to test the in-vitro susceptibility of the identified isolates to different antibiotics. Results: The non-lactose fermenting gram negative bacteria were isolated from samples of blood (33.30%), pus/ wound (33.30%), urine (23.30%) and from ascetic/pleural fluids (10.20%). The study revealed that Pseudomonas aeroginosa showed high resistance against Gentamicin (74%) and Aztreonam (74%), followed by Ciprofloxacin (59.20%) and Amikacin (33.30). Tazocin was active as low resistance (18.50%) is shown. More resistance was seen in Morganella morganii against Aztreonam (77.7%) followed by Gentamicin (62.90%), Ciprofloxacin (40.70%). Tazocin show low resistance (3.70%). Multidrug resistant Proteus mirabillis was highly resistance to Gentamicin (66.60%), followed by Aztreonam (62.90%), Amikacin (55.50%), Ciprofloxacin (40.20%) and low resistance to Tazocin was (22.20%). Salmonella typhi demonstrated high resistance against Amikacin (62.90%), followed by Aztreonam (48.10%), Tazocin (40.70%). Gentamicin showed low resistance (29.60%), and hence it is more active against S. typhi. Conclusions: It can be concluded from the present study that different species of non-lactose fermenting gram negative bacteria have shown a different resistivity pattern. This study is a gate way for better and suitable management strategy for the infections caused by non-Lactose fermenting bacteria in the sampling region.

Contribution of soil and foliar fertilization of nitrogen and sulfur on physiological and quality assessment of wheat (<i>Triticum aestivum</i>L.)

Nitrogen and sulfur supplies have a strong influence on the physical characteristics of crop as well as on the quality and quantity of wheat storage proteins, which play an important role in bread-making process. In order to evaluate the contribution of soil and foliar fertilization of nitrogen and sulfur on physiological and quality assessment of wheat, a field trail was carried out having randomized complete block design with four replications and eight different treatments of nitrogen and sulfur combinations were allotted to plots at different growth stages. Results indicated that highest protein content (12.82%), maximum moisture content (10.9%), maximum crop growth rate and maximum absolute growth rate were recorded when the wheat crop was fertilized with T8 [Nitrogen @ 60 kg·ha-1 at sowing + 40 kg·ha-1 at tillering + 10 kg·ha-1 at anthesis (spray) + 10 kg·ha-1 after anthesis (spray)] + [Sulfur @ 15 kg·ha-1 at sowing + 10 kg·ha-1 at anthesis (spray) + 5 kg·ha-1 after anthesis (spray)], while control practice resulted low moisture content, low protein, minimum crop growth rate and low absolute growth rate. Among physiological components of wheat cultivars, leaf area index was enhanced when fertilization was done with T5 (Sulfur @ 15 kg·ha-1 at sowing + 10 kg·ha-1 at anthesis + 5 kg·ha-1 after anthesis). In all the recorded observations,concerning experiment wheat cultivar Pirsabaq2005 showed appreciable response as compared with other variety (Khyber-87). Thus it is possible to obtain maximum physiological traits as well as bread-making quality of wheat through soil and foliar application of nitrogen and sulfur.