Meromictic soda and saline lakes are unique ecosystems characterized by the stability of physical,chemical and biological parameters,and they are distributed all over the world.Lakes located in regions with average an...Meromictic soda and saline lakes are unique ecosystems characterized by the stability of physical,chemical and biological parameters,and they are distributed all over the world.Lakes located in regions with average annual negative air temperature are of particular interest because of the presence of two periods with intensive and dynamic processes:the so-called biological summer and the long ice season with the biological spring.Soda Lake Doroninskoe is located in Eastern Transbaikalia(51°14′N,112°14′E) in the permafrost zone in an extreme continental climate,and is covered by ice for seven months per year.The structure and diversity of the microbial communities throughout the water column of the lake was studied by 16 S r RNA gene amplicon metasequencing.Different species with specific functions were found to dominate at different depths.Metabolically flexible bacteria with a capacity to switch between anoxygenic photosynthesis and aerobic chemotrophic metabolism dominate in soda Lake Doroninskoe.展开更多
Extreme environmental conditions are key factors in the formation of the structure and diversity of microbial communities. In meromictic ecosystems, extreme conditions and a stable stratification of physical, chemical...Extreme environmental conditions are key factors in the formation of the structure and diversity of microbial communities. In meromictic ecosystems, extreme conditions and a stable stratification of physical, chemical and biological parameters lead to diversity and heterogeneity of microenvironments. Lake Doroninskoe is located in an extreme geographical area and diff ers from other known meromictic reservoirs of the world by a low level of illumination in the chemocline and a rare type of alkaline water in sedimentary rocks formed by evaporative concentration. To understand the key factors that shape the composition and structure of the microbial community, the macro-and micro-variations in space and time are of great importance. We investigated the short-term dynamics of the structure and diversity of microbial communities of the meromictic soda lake, Lake Doroninskoe, at day and night using highthroughput sequencing and bioinformatics. Metagenomic analysis of 16 S rRNA gene amplicons showed that the microbial communities had a high taxonomic diversity both at day and night. Sixteen bacterial and three archaeal phyla were identified. Proteobacteria were dominant and comprised 75% during the day, increasing to 90% at night. Metabolically stable denitrifying bacteria that were able to use a variety of alternative electron acceptors and electron donors were prevalent in Lake Doroninskoe. They belonged to the families Enterobacteriaceae(class Gammaproteobacteria) and Alcaligenaceae(class Betaproteobacteria). Statistically significant differences between day and night microbial communities were found. During the day, the microbial community was the most diverse. We discuss the peculiarities of the underexplored shortterm dynamics of the structure and diversity of the microbial communities of the meromictic soda Lake Doroninskoe, and propose topics for prospective studies.展开更多
1 Introduction Many soda and salt lakes are characterized by the formation of the meromictic conditions under which a part of the water column is not involved in the annual process of mixing(Mac Intyre,Melack,1982).Th...1 Introduction Many soda and salt lakes are characterized by the formation of the meromictic conditions under which a part of the water column is not involved in the annual process of mixing(Mac Intyre,Melack,1982).This creates an展开更多
1 Introduction Prokaryotes are key organisms in aquatic ecosystems as they play animportant role in the biogeochemical cycling of elements.Investigations on the relationships betweenthe diversity of microbial communit...1 Introduction Prokaryotes are key organisms in aquatic ecosystems as they play animportant role in the biogeochemical cycling of elements.Investigations on the relationships betweenthe diversity of microbial community and environmentalfactors offer useful information that bothleads to understanding of the process of element展开更多
1 Introduction Regosol in Eastern Siberia is intrazonal soil.In Priolkhonie region it occurs among chestnut soils,salt marshes and solonetzes.There are small information about Tagotskij hydrolaccolith in the research ...1 Introduction Regosol in Eastern Siberia is intrazonal soil.In Priolkhonie region it occurs among chestnut soils,salt marshes and solonetzes.There are small information about Tagotskij hydrolaccolith in the research articles of geologists,archaeologists,and paleolimnologists when展开更多
文摘Meromictic soda and saline lakes are unique ecosystems characterized by the stability of physical,chemical and biological parameters,and they are distributed all over the world.Lakes located in regions with average annual negative air temperature are of particular interest because of the presence of two periods with intensive and dynamic processes:the so-called biological summer and the long ice season with the biological spring.Soda Lake Doroninskoe is located in Eastern Transbaikalia(51°14′N,112°14′E) in the permafrost zone in an extreme continental climate,and is covered by ice for seven months per year.The structure and diversity of the microbial communities throughout the water column of the lake was studied by 16 S r RNA gene amplicon metasequencing.Different species with specific functions were found to dominate at different depths.Metabolically flexible bacteria with a capacity to switch between anoxygenic photosynthesis and aerobic chemotrophic metabolism dominate in soda Lake Doroninskoe.
基金conducted according to the project IX.137.1.1“Biodiversity of natural and natural-technogenic ecosystems of Transbaikalia(Cenrtal Asia)as indicators of regional climate changes”(АААА-А17-117011210078-9)
文摘Extreme environmental conditions are key factors in the formation of the structure and diversity of microbial communities. In meromictic ecosystems, extreme conditions and a stable stratification of physical, chemical and biological parameters lead to diversity and heterogeneity of microenvironments. Lake Doroninskoe is located in an extreme geographical area and diff ers from other known meromictic reservoirs of the world by a low level of illumination in the chemocline and a rare type of alkaline water in sedimentary rocks formed by evaporative concentration. To understand the key factors that shape the composition and structure of the microbial community, the macro-and micro-variations in space and time are of great importance. We investigated the short-term dynamics of the structure and diversity of microbial communities of the meromictic soda lake, Lake Doroninskoe, at day and night using highthroughput sequencing and bioinformatics. Metagenomic analysis of 16 S rRNA gene amplicons showed that the microbial communities had a high taxonomic diversity both at day and night. Sixteen bacterial and three archaeal phyla were identified. Proteobacteria were dominant and comprised 75% during the day, increasing to 90% at night. Metabolically stable denitrifying bacteria that were able to use a variety of alternative electron acceptors and electron donors were prevalent in Lake Doroninskoe. They belonged to the families Enterobacteriaceae(class Gammaproteobacteria) and Alcaligenaceae(class Betaproteobacteria). Statistically significant differences between day and night microbial communities were found. During the day, the microbial community was the most diverse. We discuss the peculiarities of the underexplored shortterm dynamics of the structure and diversity of the microbial communities of the meromictic soda Lake Doroninskoe, and propose topics for prospective studies.
文摘1 Introduction Many soda and salt lakes are characterized by the formation of the meromictic conditions under which a part of the water column is not involved in the annual process of mixing(Mac Intyre,Melack,1982).This creates an
基金performed as part of theintegration project of SB RAS № 56
文摘1 Introduction Prokaryotes are key organisms in aquatic ecosystems as they play animportant role in the biogeochemical cycling of elements.Investigations on the relationships betweenthe diversity of microbial community and environmentalfactors offer useful information that bothleads to understanding of the process of element
文摘1 Introduction Regosol in Eastern Siberia is intrazonal soil.In Priolkhonie region it occurs among chestnut soils,salt marshes and solonetzes.There are small information about Tagotskij hydrolaccolith in the research articles of geologists,archaeologists,and paleolimnologists when
