Hyaluronic acid(HA, 20–50 kDa) is a hydrophilic macromolecule with anti-wrinkle effects and moisturizing properties. However, its high molecular weight prevents it from penetrating into the deeper layers of the skin ...Hyaluronic acid(HA, 20–50 kDa) is a hydrophilic macromolecule with anti-wrinkle effects and moisturizing properties. However, its high molecular weight prevents it from penetrating into the deeper layers of the skin and, thus, limits its benefits to topical effects. Thus, the objective of this study is to prepare nanoparticles of quaternized cyclodextrin-grafted chitosan(QCD-g-CS) associated with HA in different molar ratios of QCD-g-CS and HA. The conjugation of the carboxylic moieties of HA and the amides of QCD-g-CS was confirmed by Fourier-transform infrared spectroscopy. Thus, the system was optimized to create nanoparticles with a small size(235.63 ± 21.89 nm), narrow polydispersity index(0.13 ± 0.02), and zeta potential of 16.07 ± 0.65 m V. The association efficiency and loading efficiency were determined by ultra-performance liquid chromatography as 86.77 ± 0.69% and 10.85 ± 0.09%, respectively. The spherical morphology of the obtained nanoparticles was confirmed by transmission electron microscopy. Moreover, the in-vitro hydrating ability was significantly higher( P < 0.001) than that of bulk HA(3.29 ± 0.41 and 1.71 ± 0.05 g water/g sample, respectively). The safety of these nanoparticles at concentrations in the range of 0.01–0.10 mg/ml was confirmed via tests on human skin fibroblasts. Together, these results demonstrate that the developed nanoparticles are promising for future applications in cosmetics.展开更多
Objective:Dendrobium spp.,the major globally commercializing tropical orchid,has been continuously used in Chinese medicine.However,preparation and standardization of this therapeutic orchid including its clinical evi...Objective:Dendrobium spp.,the major globally commercializing tropical orchid,has been continuously used in Chinese medicine.However,preparation and standardization of this therapeutic orchid including its clinical evidence for topical application are sparely to be exploited.Methods:Maceration of the white orchid(Dendrobium cv.Khao Sanan)in water was varied on the extraction time.The extraction yields and total polysaccharide content of the extracts were compared.The best extract condition was selected,standardized,and evaluated on safety and skin hydrating efficacy in human volunteers.Results:The extraction for 3 h gave the extract with polysaccharide content that significantly(P<0.001)greater than the other extraction times with the following specification:yield[(35.60±2.51)%],moisture content[(7.65±0.21)%],total polysaccharide,sugar,reducing sugar and ash content[(72.95±2.37)%,(34.38±0.00)%,(16.28±0.00)%,and(0.12±0.00)%],p H(4.42±0.01),viscosity[(15.07±0.12)cps]swelling and water absorption capacities[(10.00±0.00)%,(1.19±0.24)g/g].This antioxidative polysaccharide caused no skin irritation with a better skin hydrating efficacy than the untreated skin and the benchmark as examined in 22 Thai volunteers.Conclusion:White Dendrobium is ready to be supplied as a specialty ingredient with a safe and efficient profile for skin dryness therapy.This innovative application of the identified traditional Chinese medicinal herb flows in the main stream of the consumers’preferences and demand upon natural derived products.展开更多
基金Mae Fah Luang University is acknowledged for facilities and financially supported in partfunded by Thailand Graduate Institute of Science and Technology(TGIST),National Science and Technology De-velopment Agency(NSTDA),Thailand(Project No.SCA-CO-2558-1026-TH)
文摘Hyaluronic acid(HA, 20–50 kDa) is a hydrophilic macromolecule with anti-wrinkle effects and moisturizing properties. However, its high molecular weight prevents it from penetrating into the deeper layers of the skin and, thus, limits its benefits to topical effects. Thus, the objective of this study is to prepare nanoparticles of quaternized cyclodextrin-grafted chitosan(QCD-g-CS) associated with HA in different molar ratios of QCD-g-CS and HA. The conjugation of the carboxylic moieties of HA and the amides of QCD-g-CS was confirmed by Fourier-transform infrared spectroscopy. Thus, the system was optimized to create nanoparticles with a small size(235.63 ± 21.89 nm), narrow polydispersity index(0.13 ± 0.02), and zeta potential of 16.07 ± 0.65 m V. The association efficiency and loading efficiency were determined by ultra-performance liquid chromatography as 86.77 ± 0.69% and 10.85 ± 0.09%, respectively. The spherical morphology of the obtained nanoparticles was confirmed by transmission electron microscopy. Moreover, the in-vitro hydrating ability was significantly higher( P < 0.001) than that of bulk HA(3.29 ± 0.41 and 1.71 ± 0.05 g water/g sample, respectively). The safety of these nanoparticles at concentrations in the range of 0.01–0.10 mg/ml was confirmed via tests on human skin fibroblasts. Together, these results demonstrate that the developed nanoparticles are promising for future applications in cosmetics.
基金Mae Fah Luang University is acknowledged upon financial and facilities supports during this manuscript preparation.
文摘Objective:Dendrobium spp.,the major globally commercializing tropical orchid,has been continuously used in Chinese medicine.However,preparation and standardization of this therapeutic orchid including its clinical evidence for topical application are sparely to be exploited.Methods:Maceration of the white orchid(Dendrobium cv.Khao Sanan)in water was varied on the extraction time.The extraction yields and total polysaccharide content of the extracts were compared.The best extract condition was selected,standardized,and evaluated on safety and skin hydrating efficacy in human volunteers.Results:The extraction for 3 h gave the extract with polysaccharide content that significantly(P<0.001)greater than the other extraction times with the following specification:yield[(35.60±2.51)%],moisture content[(7.65±0.21)%],total polysaccharide,sugar,reducing sugar and ash content[(72.95±2.37)%,(34.38±0.00)%,(16.28±0.00)%,and(0.12±0.00)%],p H(4.42±0.01),viscosity[(15.07±0.12)cps]swelling and water absorption capacities[(10.00±0.00)%,(1.19±0.24)g/g].This antioxidative polysaccharide caused no skin irritation with a better skin hydrating efficacy than the untreated skin and the benchmark as examined in 22 Thai volunteers.Conclusion:White Dendrobium is ready to be supplied as a specialty ingredient with a safe and efficient profile for skin dryness therapy.This innovative application of the identified traditional Chinese medicinal herb flows in the main stream of the consumers’preferences and demand upon natural derived products.
