精准皮肤诊疗室——皮肤镜显微镜工作平台

建立皮肤镜-显微镜工作平台,将临床诊断、实验室检查和治疗等环节有机整合,是皮肤疾病精准诊疗的关键。"精准皮肤诊疗室"将诊断室、病原体检查室、病理室等相关功能集中、浓缩与结合,一体化同步完成,主要设备为皮肤镜和显微镜,初衷和最终目的是建立一支学习性临床研究团队。"精准皮肤诊疗室"从概念到实践不断完善其内涵。

Characterization of chickpea germplasm conserved in the Indian National Genebank and development of a core set using qualitative and quantitative trait data

Chickpea is the third most important pulse crop as a source of dietary protein. Ever-increasing demand in Asian countries calls for breeding superior desi-type varieties, in turn necessitating the availability of characterized germplasm to breeders. The Indian National Genebank,located at the National Bureau of Plant Genetic Resources, New Delhi, conserves 14,651 accessions of chickpea. The entire set was characterized in a single large-scale experiment.High variation was observed for eight quantitative and 12 qualitative agro-morphological traits. Allelic richness procedure was employed to assemble a core set comprising 1103 accessions, 70.0% of which were of Indian origin. Comparable values of total variation explained by the first three principal components in the entire collection(51.1%) and the core(52.4%)together with conservation of nine pairwise r values among quantitative traits in the core collection and a coincidence rate around 99.7% indicated that the chickpea core was indeed an excellent representation of the entire chickpea collection in the National Genebank. The chickpea core exhibited greater diversity than the entire collection in agro-morphological traits, as assessed by higher variance and Shannon–Weaver diversity indices, indicating that the chickpea core maximized the phenotypic diversity available in the Indian chickpea germplasm. The chickpea core, comprising mainly indigenous desi genotypes, is expected to be an excellent resource for chickpea breeders. Information on the chickpea core can be accessed at http://www.nbpgr.ernet.in/pgrportal.

Mild oxidative stress is beneficial for sperm telomere length maintenance

AIM:To evaluate telomere length in sperm DNA and its correlation with oxidative stress(normal,mild,severe).METHODS:The study included infertile men(n=112)and age matched fertile controls(n=102).The average telomere length from the sperm DNA was measured using a quantitative real time PCR based assay.Seminal reactive oxygen species(ROS)and 8-Isoprostane(8-IP)levels were measured by chemiluminescence assay and ELISA respectively.RESULTS:Average sperm telomere length in infertile men and controls was 0.609±0.15 and 0.789±0.060,respectively(P<0.0001).Seminal ROS levels in infertile was higher[66.61±28.32 relative light units(RLU)/s/million sperm]than in controls(14.04±10.67RLU/s/million sperm)(P<0.0001).The 8-IP level in infertile men was significantly higher(421.55±131.29pg/mL)than in controls(275.94±48.13 pg/mL)(P<0.001).When correlated to oxidative stress,in normal range of oxidative stress(ROS,0-21.3 RLU/s/million sperm)the average telomere length in cases was 0.663±0.14,in mild oxidative stress(ROS,21.3-35 RLU/s/million sperm)it was elevated(0.684±0.12)and in severe oxidative stress(ROS>35 RLU/s/million sperm)average telomere length was decreased to 0.595±0.15.CONCLUSION:Mild oxidative stress results in lengthening of telomere length,but severe oxidative stress results in shorter telomeres.Although telomere maintenance is a complex trait,the study shows that mild oxidative stress is beneficial in telomere length maintenance and thus a delicate balance needs to be established to maximize the beneficial effects of free radicals and prevent harmful effects of supra physiological levels.Detailed molecular evaluation of telomere structure,its correlation with oxidative stress would aid in elucidating the cause of accelerated telomere length attrition.

Impact of geographical and seasonal temperature on sperm parameters in Indian men who were partners in subfertile couples-A retrospective analysis

Objective:To evaluate the impact of seasonal and geographical temperature on sperm parameters in Indian men who were partners in subfertile couples.Methods:A retrospective review of the records of semen analysis from January 2009 to December 2017 that was performed as part of primary evaluation of infertile couple was undertaken.Mean values of various semen parameters were compared between seasons using one-way analysis of variance test followed by Bonferroni pair wise post-hoc comparison test.Bivariate Pearson correlation coefficient was applied to measure the correlation between age,sperm parameters and annual temperature/seasons.The logistic regression analysis was further carried out to identify significant variables contributing to normal progressive motility of sperm.Results:A total of 3433 reports of semen samples were analysed.There was no significant seasonal variation in the mean volume,concentration,and total motility levels(P>0.05)except the mean values of progressive motility(P<0.001).The mean values of progressive motility during pre-monsoon were significantly higher compared to the corresponding values during monsoon and autumn seasons(P<0.01).In all the seasons,total motility had significant positive correlation with sperm concentration and progressive motility(P<0.05).Pre-monsoon emerged as the significant favourable season to get normal progressive motility level(OR 1.32,95%CI 1.04-1.68)compared to winter.The chances of getting normal progressive motility reduced due to longer abstinence period(OR 0.87,95%CI 0.80-0.93).When the temperature was more than 28℃,the likelihood of getting normal progressive motility decreased(OR 0.70,95%CI 0.51-0.95).Conclusions:The progressive motility of sperms significantly decreases from pre-monsoon to autumn with recovery towards winter,which substantiates the effect of rising temperature on the progressive motility of sperm.This is clinically important to counsel infertile patients,so that they can plan their treatment accordingly.