We report the isolation of AtL1, a 249 bp non-LTR retrotransposon fragment from Arabidopsis thaliana by fingerprinting mRNAs extracted from A. thaliana plants, ecotype Columbia, in different heat stress conditions. So...We report the isolation of AtL1, a 249 bp non-LTR retrotransposon fragment from Arabidopsis thaliana by fingerprinting mRNAs extracted from A. thaliana plants, ecotype Columbia, in different heat stress conditions. Southern blot and PCR analysis suggested that AtL1 occurs as a single- or low-copy insert in the genome of A. thaliana ecotype Columbia. The presence of AtL1 in the genome of different Arabidopsis ecotypes was confirmed by PCR amplification and sequencing thus excluding all possible contamination. A preliminary scan of the AtL1 nucleotide sequence against the EMBL and NCBI databases revealed a high degree of similarity to a group of LINE type L1 retrotransposons of mammals and with a cDNA sequence of Artemisia annua. A phylogenetic analysis of LINE elements from animals and plants placed AtL1 and A. annua sequences in close proximity to some mammalian sequences but distant from the other plants LINE elements including those from Arabidopsis.展开更多
Drought stress imposes a major constraint over a crop yield and can be expected to grow in importance if the climate change predicted comes about.Improved methods are needed to facilitate crop management via the promp...Drought stress imposes a major constraint over a crop yield and can be expected to grow in importance if the climate change predicted comes about.Improved methods are needed to facilitate crop management via the prompt detection of the onset of stress.Here,we report the use of an in vivo OECT(organic electrochemical transistor)sensor,termed as bioristor,in the context of the drought response of the tomato plant.The device was integrated within the plant’s stem,thereby allowing for the continuous monitoring of the plant’s physiological status throughout its life cycle.Bioristor was able to detect changes of ion concentration in the sap upon drought,in particular,those dissolved and transported through the transpiration stream,thus efficiently detecting the occurrence of drought stress immediately after the priming of the defence responses.The bioristor’s acquired data were coupled with those obtained in a high-throughput phenotyping platform revealing the extreme complementarity of these methods to investigate the mechanisms triggered by the plant during the drought stress event.展开更多
文摘We report the isolation of AtL1, a 249 bp non-LTR retrotransposon fragment from Arabidopsis thaliana by fingerprinting mRNAs extracted from A. thaliana plants, ecotype Columbia, in different heat stress conditions. Southern blot and PCR analysis suggested that AtL1 occurs as a single- or low-copy insert in the genome of A. thaliana ecotype Columbia. The presence of AtL1 in the genome of different Arabidopsis ecotypes was confirmed by PCR amplification and sequencing thus excluding all possible contamination. A preliminary scan of the AtL1 nucleotide sequence against the EMBL and NCBI databases revealed a high degree of similarity to a group of LINE type L1 retrotransposons of mammals and with a cDNA sequence of Artemisia annua. A phylogenetic analysis of LINE elements from animals and plants placed AtL1 and A. annua sequences in close proximity to some mammalian sequences but distant from the other plants LINE elements including those from Arabidopsis.
基金This work was partially funded by RGV FAO DM 10271.
文摘Drought stress imposes a major constraint over a crop yield and can be expected to grow in importance if the climate change predicted comes about.Improved methods are needed to facilitate crop management via the prompt detection of the onset of stress.Here,we report the use of an in vivo OECT(organic electrochemical transistor)sensor,termed as bioristor,in the context of the drought response of the tomato plant.The device was integrated within the plant’s stem,thereby allowing for the continuous monitoring of the plant’s physiological status throughout its life cycle.Bioristor was able to detect changes of ion concentration in the sap upon drought,in particular,those dissolved and transported through the transpiration stream,thus efficiently detecting the occurrence of drought stress immediately after the priming of the defence responses.The bioristor’s acquired data were coupled with those obtained in a high-throughput phenotyping platform revealing the extreme complementarity of these methods to investigate the mechanisms triggered by the plant during the drought stress event.
