Three simple,specific,accurate and precise spectrophotometric methods are developed for simultaneous determination of amlodipine besylate(AM)and atenolol(AT)in tablets.The first method is dual wavelength spectrophotom...Three simple,specific,accurate and precise spectrophotometric methods are developed for simultaneous determination of amlodipine besylate(AM)and atenolol(AT)in tablets.The first method is dual wavelength spectrophotometry(DW).The second method is ratio subtraction(RS)which depends on subtraction of the plateau values from the ratio spectrum,coupled to first derivative of ratio spectra(1 DD).The third method applies bivariate calibration method using 210and 225nm as an optimum pair of wavelength for amlodipine and atenolol.The calibration curves are linear over the concentration range of 4~40μg·mL-1 for both drugs.The specificity of the developed methods is investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form.The two methods are validated as per ICH guidelines and can be applied for routine quality control testing.展开更多
A simple,specific,accurate and precise spectrophotometric stability indicating method is developed for determination of itraconazole in the presence of its oxidative degradation product and in pharmaceutical formulati...A simple,specific,accurate and precise spectrophotometric stability indicating method is developed for determination of itraconazole in the presence of its oxidative degradation product and in pharmaceutical formulations.A newly developed spectrophotometric method called ratio difference method by measuring the difference in amplitudes between 230 and 265nm of ratio spectra.The calibration curve is linear over the concentration range of 5~25μg·mL-1 with mean percentage recovery of 99.81±1.002.Selective quantification of itraconazole,singly in bulk form,pharmaceutical formulations and in the presence of its oxidative degradation product is demonstrated.The results have been statistically compared with a pharmacopeial method.展开更多
A simple,specific,accurate and precise spectrophotometric stability indicating method is developed for determination of bambuterol hydrochloride(BH)in the presence of its degradation product terbutaline(TERB)and in ph...A simple,specific,accurate and precise spectrophotometric stability indicating method is developed for determination of bambuterol hydrochloride(BH)in the presence of its degradation product terbutaline(TERB)and in pharmaceutical formulations.A newly developed spectrophotometric method called ratio difference method by measuring the difference in amplitudes between 245 and 260nm of ratio spectra.The calibration curves are linear over the concentration range of 0.1~1mg·mL-1 for BH and 0.1~0.7mg·mL-1 for TERB with mean percentage recovery of 100.56±0.751 and 99.88±1.183,respectively.The selectivity of the proposed method is checked using laboratory prepared mixtures.The proposed method has been successfully applied to the analysis of BH in pharmaceutical dosage forms without interference from other dosage form additives and the results have been statistically compared with pharmacopeial method.展开更多
文摘Three simple,specific,accurate and precise spectrophotometric methods are developed for simultaneous determination of amlodipine besylate(AM)and atenolol(AT)in tablets.The first method is dual wavelength spectrophotometry(DW).The second method is ratio subtraction(RS)which depends on subtraction of the plateau values from the ratio spectrum,coupled to first derivative of ratio spectra(1 DD).The third method applies bivariate calibration method using 210and 225nm as an optimum pair of wavelength for amlodipine and atenolol.The calibration curves are linear over the concentration range of 4~40μg·mL-1 for both drugs.The specificity of the developed methods is investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form.The two methods are validated as per ICH guidelines and can be applied for routine quality control testing.
文摘A simple,specific,accurate and precise spectrophotometric stability indicating method is developed for determination of itraconazole in the presence of its oxidative degradation product and in pharmaceutical formulations.A newly developed spectrophotometric method called ratio difference method by measuring the difference in amplitudes between 230 and 265nm of ratio spectra.The calibration curve is linear over the concentration range of 5~25μg·mL-1 with mean percentage recovery of 99.81±1.002.Selective quantification of itraconazole,singly in bulk form,pharmaceutical formulations and in the presence of its oxidative degradation product is demonstrated.The results have been statistically compared with a pharmacopeial method.
文摘A simple,specific,accurate and precise spectrophotometric stability indicating method is developed for determination of bambuterol hydrochloride(BH)in the presence of its degradation product terbutaline(TERB)and in pharmaceutical formulations.A newly developed spectrophotometric method called ratio difference method by measuring the difference in amplitudes between 245 and 260nm of ratio spectra.The calibration curves are linear over the concentration range of 0.1~1mg·mL-1 for BH and 0.1~0.7mg·mL-1 for TERB with mean percentage recovery of 100.56±0.751 and 99.88±1.183,respectively.The selectivity of the proposed method is checked using laboratory prepared mixtures.The proposed method has been successfully applied to the analysis of BH in pharmaceutical dosage forms without interference from other dosage form additives and the results have been statistically compared with pharmacopeial method.
