The effects of low energy X-ray irradiation on microbial inactivation,sprouting inhibition and genetic variation were surveyed.There was an inverse correlation between the exposure dose in range of 0 Gy–5000...The effects of low energy X-ray irradiation on microbial inactivation,sprouting inhibition and genetic variation were surveyed.There was an inverse correlation between the exposure dose in range of 0 Gy–5000 Gy and the survival rate of microorganisms.The dose of 1000 Gy seemed to be suitable for inactivation of microorganisms because the respective survival rate is relatively low (0.59%).Dose rate of 13.87 Gy/min was the most appropriate to inactivate the microorganisms,with the survival rate of 0.37%.Applying the irradiation dose of 1000 Gy and dose rate of 13.87 Gy/min,there was a significant sprouting inhibition of irradiated potatoes compared to the control for six months storage.The effect of irradiation dose (100–1200 Gy) and dose rate (8.63–19.62 Gy/min) on genetic variation of the irradiated samples was assessed by DNA fingerprints induced from using three molecular techniques including Random Amplified Polymorphic DNA (RAPD),Start Codon Targeted Polymorphism (SCoT) and CAAT box – derived polymorphism (CBDP).At the irradiation dose of 1000 Gy and dose rate of 13.87 Gy/min,the level of genetic variation was relatively low (4.85%).Thus,this treatment is a suitable condition of irradiation for potato treatment with the purpose of preservation.展开更多
文摘The effects of low energy X-ray irradiation on microbial inactivation,sprouting inhibition and genetic variation were surveyed.There was an inverse correlation between the exposure dose in range of 0 Gy–5000 Gy and the survival rate of microorganisms.The dose of 1000 Gy seemed to be suitable for inactivation of microorganisms because the respective survival rate is relatively low (0.59%).Dose rate of 13.87 Gy/min was the most appropriate to inactivate the microorganisms,with the survival rate of 0.37%.Applying the irradiation dose of 1000 Gy and dose rate of 13.87 Gy/min,there was a significant sprouting inhibition of irradiated potatoes compared to the control for six months storage.The effect of irradiation dose (100–1200 Gy) and dose rate (8.63–19.62 Gy/min) on genetic variation of the irradiated samples was assessed by DNA fingerprints induced from using three molecular techniques including Random Amplified Polymorphic DNA (RAPD),Start Codon Targeted Polymorphism (SCoT) and CAAT box – derived polymorphism (CBDP).At the irradiation dose of 1000 Gy and dose rate of 13.87 Gy/min,the level of genetic variation was relatively low (4.85%).Thus,this treatment is a suitable condition of irradiation for potato treatment with the purpose of preservation.
