To investigate the expression of Prominin-1(Prom-1)in mouse uterus during peri-implantation.In situ hybridization and immunohistochemical staining were used to detect the mRNA and protein expression level of Prom-1 in...To investigate the expression of Prominin-1(Prom-1)in mouse uterus during peri-implantation.In situ hybridization and immunohistochemical staining were used to detect the mRNA and protein expression level of Prom-1 in mice uterus in early pregnancy,pseudopregnancy,estrous cycle,treated with hormone,delayed implantation and activation models.The results showed that Prom-1 was gradually weakened in uterine luminal epithelium(LE)and glandular epithelium(GE)during days 1-4 of pregnancy.During days 5-8,Prom-1 was strongly expressed in GE,and signal of Prom-1 protein was detected in matrix and decidua around the embryo.Similar to pregnancy,Prom-1 was strongly expressed in LE and GE on the 1st day and weakly expressed on the 4th day of pseudopregnancy.In addition,Prom-1 was highly expressed in LE and GE on estrus.Expression of Prom-1 was observed in the LE and the GE of delayed-implantation uterus.In activated-implantation animal model,Prom-1 was strongly expressed in the GE.In the hormone-treated model,Prom-1 expression levels were higher in the uterus of the 17β-estradiol-treated group than those in the control group.These results indicated that Prom-1 might promote the proliferation of mouse endometrial epithelium and participate in the establishment of uterine receptivity.Meanwhile,the expression of Prom-1 was up-regulated by the 17β-estradiol,indicating that Prom-1 might involve in the process of decidua development regulated by uterine glands,and Prom-1 might play an important role in mice early pregnancy.展开更多
目的:探讨约束决策轮对急诊重症监护室(Emergency Intensive Care Unit,EICU)患者身体约束情况的应用效果。方法:选择2017年9月—2019年2月南通大学附属医院收治的EICU患者为研究对象,按照入院时间将患者分为干预组(n=199)和对照组(n=15...目的:探讨约束决策轮对急诊重症监护室(Emergency Intensive Care Unit,EICU)患者身体约束情况的应用效果。方法:选择2017年9月—2019年2月南通大学附属医院收治的EICU患者为研究对象,按照入院时间将患者分为干预组(n=199)和对照组(n=157),干预组通过约束决策轮评估对患者实施必要性身体约束,对照组通过《约束护理单》对患者实施身体约束。结果:两组身体约束时间和身体约束率比较差异均有统计学意义(均P<0.05);干预组非计划性拔管率虽有降低,但差异无统计学意义(P>0.05);两组患者约束期均无皮肤损伤。结论:应用约束决策轮对EICU患者进行身体约束评估,可明显减少约束时间,有效降低身体约束率,保障患者的安全。展开更多
基金Supported by the National Natural Science Foundation of China(31472097)
文摘To investigate the expression of Prominin-1(Prom-1)in mouse uterus during peri-implantation.In situ hybridization and immunohistochemical staining were used to detect the mRNA and protein expression level of Prom-1 in mice uterus in early pregnancy,pseudopregnancy,estrous cycle,treated with hormone,delayed implantation and activation models.The results showed that Prom-1 was gradually weakened in uterine luminal epithelium(LE)and glandular epithelium(GE)during days 1-4 of pregnancy.During days 5-8,Prom-1 was strongly expressed in GE,and signal of Prom-1 protein was detected in matrix and decidua around the embryo.Similar to pregnancy,Prom-1 was strongly expressed in LE and GE on the 1st day and weakly expressed on the 4th day of pseudopregnancy.In addition,Prom-1 was highly expressed in LE and GE on estrus.Expression of Prom-1 was observed in the LE and the GE of delayed-implantation uterus.In activated-implantation animal model,Prom-1 was strongly expressed in the GE.In the hormone-treated model,Prom-1 expression levels were higher in the uterus of the 17β-estradiol-treated group than those in the control group.These results indicated that Prom-1 might promote the proliferation of mouse endometrial epithelium and participate in the establishment of uterine receptivity.Meanwhile,the expression of Prom-1 was up-regulated by the 17β-estradiol,indicating that Prom-1 might involve in the process of decidua development regulated by uterine glands,and Prom-1 might play an important role in mice early pregnancy.
文摘目的:探讨约束决策轮对急诊重症监护室(Emergency Intensive Care Unit,EICU)患者身体约束情况的应用效果。方法:选择2017年9月—2019年2月南通大学附属医院收治的EICU患者为研究对象,按照入院时间将患者分为干预组(n=199)和对照组(n=157),干预组通过约束决策轮评估对患者实施必要性身体约束,对照组通过《约束护理单》对患者实施身体约束。结果:两组身体约束时间和身体约束率比较差异均有统计学意义(均P<0.05);干预组非计划性拔管率虽有降低,但差异无统计学意义(P>0.05);两组患者约束期均无皮肤损伤。结论:应用约束决策轮对EICU患者进行身体约束评估,可明显减少约束时间,有效降低身体约束率,保障患者的安全。
