Research progress in spasmodic torticollis rehabilitation treatment

Spasmodic torticollis(ST)is a focal dystonia that affects adults,causing limited muscle control and impacting daily activities and quality of life.The etiology and curative methods for ST remain unclear.Botulinum toxin is widely used as a firstline treatment,but long-term usage can result in reduced tolerance and adverse effects.Rehabilitation therapy,with its minimal side effects and low potential for harm,holds significant clinical value.This article explores the effectiveness of adjunctive therapies,including exercise therapy,transcranial magnetic stimulation,shockwave therapy,neuromuscular electrical stimulation,vibration therapy,electromyographic biofeedback,and acupuncture,in the treatment of ST.The aim is to provide clinicians with additional treatment options and to discuss the efficacy of rehabilitation therapy for ST.

支化聚乙烯醇水溶液的流变行为

以线型聚乙烯醇为参照,研究了支化程度不同的2组聚乙烯醇水溶液的稳态和动态流变行为。稳态流变结果表明,在相同条件下,聚乙烯醇(PVA)水溶液的表观黏度随主链支化程度的提升而下降,随剪切速率(10^(-1)～10~2 s^(-1))增加而下降,呈现出剪切变稀的非牛顿流体特性。同时,支化程度增加,PVA水溶液表观黏度的剪切敏感性上升。动态流变结果表明,PVA水溶液的复数黏度和松弛时间与PVA主链支化程度密切相关,提升主链支化程度,有助于降低PVA水溶液的复数黏度及松弛时间。

Prevention and Treatment of COVID-19 and Rehabilitation of Kidney,Liver and Cardiovascular Diseases with Kyllinga brevifolia Rottb.

The COVID-19 epidemic is raging,and the virus damages the lung,kidney,liver,and cardiovascular system.Medicine for prevention and treatment of COVID-19 requires three properties:being able to efficiently inhibit COVID-19 from infecting cells;being able to quickly repair lung damage;non-toxic.Kyllinga brevifolia Rottb.is found to have the three properties mentioned above and can be used for prevention,treatment and rehabilitation.In terms of rehabilitation of lung,kidney,liver,and cardiovascular diseases,K.brevifolia Rottb.shows remarkable efficacy when combined with other medicinal materials.This is instructive and useful for the rehabilitation of lung,liver,kidney,and cardiovascular damage caused by COVID-19.

Aptasensor Based on Screen-Printed Carbon Electrodes Modified with CS/AuNPs for Sensitive Detection of Okadaic Acid in Shellfish

Okadaic acid(OA),a small molecule substance derived from shellfish,is one of the most widely distributed marine toxins with acute symptoms of vomiting and diarrhea after accidental ingestion.For this,there is an urgently need for sensitive and reliable methods to detect OA in real shellfish samples.In this study,a simple aptasensor based on screen-printed carbon electrode(SPCE)with modification of chitosan(CS)and gold nanoparticles(Au NPs)was designed for electrochemical determination of OA,and the electrode surface was modified with Au NPs by potential-sweeping electrodeposition,which greatly improved the electrochemical response.The entire detection and characterization process were carried out by cyclic voltammetry(CV)with a linear correlation in the range of 0.01-100 ng/m L and a limit of detection(LOD)of 6.7 pg/m L.Furthermore,recovery rates of 92.3-116%were obtained demonstrating excellent accuracy through the recovery trial of mussel and scallop samples.

In situ self-assembly of amphiphilic dextran micelles and superparamagnetic iron oxide nanoparticle-loading as magnetic resonance imaging contrast agents

Polymeric micelles have long been considered as promising nanocarrier for hydrophobic drugs and imaging probes,due to their nanoscale particle size,biocompatibility and ability to loading reasonable amount of cargoes.Herein,a facile method for dextran micelles preparation was developed and their performance as carriers of superparamagnetic iron oxide(SPIO)nanocrystals was evaluated.Amphiphilic dextran(Dex-g-OA)was synthesized via the Schiff base reactions between oxidized dextran and oleylamine,and self-assembled in situ into nano-size micelles in the reaction systems.The self-assembling behaviors of the amphiphilic dextran were identified using fluorescence resonance energy transfer technique by detection the energy transfer signal between the fluorophore pairs,Cy5 and Cy5.5.Hydrophobic SPIO nanoparticles(Fe_(3)O_(4)NPs)were successfully loaded into the dextran micelles via the in situ self-assembly process,leading to a series of Fe_(3)O_(4)NPs-loaded micelle nanocomposites(Fe_(3)O_(4)@Dex-g-OA)with good biocompatibility,superparamagnetism and strongly enhanced T_(2)relaxivity.At the magnetic field of 0.5 T,the Fe_(3)O_(4)@Dex-g-OA nanocomposite with particle size of 116.2±53.7 nm presented a higher T_(2)relaxivity of 327.9 mM_(re)^(-1)·s^(-1)·s^(−1).The prepared magnetic nanocomposites hold the promise to be used as contrast agents in magnetic resonance imaging.

Carbon nanotube-carbon black/CaCu_(3)Ti_(4)O_(12) ternary metacomposites with tunable negative permittivity and thermal conductivity fabricated by spark plasma sintering

Metacomposites with negative permittivity usually possess huge dielectric loss,showing potential for micro-wave attenuation devices where huge heat would generate.Herein,carbon nanotube-carbon black/CaCu_(3-)Ti_(4)O_(12)(CNT-CB/CCTO)ternary metacomposites were fabricated by spark plasma sintering.The CNT-CB dualphase filler was pre-pared through electrostatic selfassembly process in order to construct an effective 3-dimensional(3D)carbon network in CCTO matrix.The percolation threshold of CNT-CB/CCTO composites was identified at filler content of 12.52 wt%which accompanied with an essential change of conduction mechanism.The negative permittivity was derived from low-frequency plasmonic state of the 3D carbon network,described by Drude model.The problem of heat transport,generally occurring in negative permittivity materials,has been solved and optimized in obtained ternary metacomposites beneftting from the substantially high thermal conductivity(9.49-2.00 W·m^(-1)·K^(-1))and diffusivity(2.74-1.22mm^(2)·s^(-1)).This work could spark significant development of practical application of metacomposites on novel electronic devices and electromagnetic apparatus.

腭裂手术模拟训练的研究进展

腭裂是常见的颌面部先天性发育缺陷,表现为腭部组织结构的不连续性,导致食物反流、发音不清、听力受损等问题,严重影响患者的生存质量。目前,手术治疗是腭裂的主要治疗手段,是颌面外科最重要的一类手术。然而,腭裂手术复杂、手术视野范围小,给手术观摩和学习带来了极大的挑战,且初学者很难在真人患者上进行训练。因此,需要腭裂手术模拟训练帮助口腔医学生了解腭裂的病理结构,掌握腭裂手术流程和手术技法。本文将对目前的腭裂手术模拟器做一综述,旨在为临床上腭裂手术教学和训练提供参考。

Harnessing stem cell and lineage reprogramming technology to treat cardiac fibrosis

Cardiac fibrosis is a pathological response characterized by excessive deposition of fibrous connective tissue within the heart.It typically occurs following cardiac injuries or diseases.However,the lack of suitable models for disease modeling and high-throughput drug discovery has hindered the establishment of an effective treatments for cardiac fibrosis.The emergence and rapid progress of stem-cell and lineage reprogramming technology offer an unprecedented opportunity to develop an improved humanized and patient-specific model for studying cardiac fibrosis,providing a platform for screening potential drugs and synchronously elucidating the underlying molecular mechanisms.Furthermore,reprogramming cardiac fibroblasts into cardiomyocyte-like cells to reduce scar volume and induce myocardial tissue regeneration is a promising approach in treating cardiac fibrosis.In this review,we summarize the current advancements in stem cell technologies applied to study cardiac fibrosis and provide insights for future investigations into its mechanisms,drug discovery as well as therapy method.

Changes in fruit pigment accumulation,chloroplast development,and transcriptome analysis in the CRISPR/Cas9-mediated knockout of Stay-green 1(slsgr1)mutant

The green-flesh(gf)mutant of the tomato fruit ripen to a muddy brown color and has been demonstrated previously to be a loss-of-function mutant.Here,we provide more evidence to support this view that S/SGR1 is involved in color change in ripening tomato fruits.Knocking out SISGR1 expression using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 genome editing strategy showed obviously a muddy brown color with significantly higher chlorophyll and carotenoid content compared with wild-type(WT)fruits.To further verify the role of SiSGR1 in fruit color change,we performed transcriptome deep sequencing(RNA-seq)analysis,where a total of 354 differentially expressed genes(124/230 downregulated/upregulated)were identified between WT and slsgr1.Additionally,the expression of numerous genes associated with photosynthesis and chloroplast function changed significantly when S/SGR1 was knocked out.Taken together,these results indicate that S/SGR1 is involved in color change in ripening fruit via chlorophyll degradation and carotenoid biosynthesis.