Mechanism of Cinnamomum camphora essential oil for analgesia based on gas chromatography-mass spectrometry integrated network pharmacology

This study aims to explore the analgesic ingredients and mechanism of Cinnamomum camphora essential oil(CCEO).The constituents in CCEO were characterized qualitatively by gas chromatography-mass spectrometry.Targets related to active ingredients were collected by PubChem and Swiss Target Prediction.Targets related to pain were screened by TTD and OMIM database,and compound-target network was established by Cytoscape software.Gene ontology(GO)function and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis of targets were carried out by DAVID database.Protein-protein interaction(PPI)network was established and analyzed by STRING database.Molecular docking method was used to verify the interaction between main components and relevant core targets.A total of 13 compounds were identified in CCEO,and 58 related targets were predicted.GO function enrichment analysis revealed that the selected targets were mainly involved in biological processes such as chemical synaptic transmission and molecular function such as neurotransmitter receptor activity;24 signal pathways were screened by KEGG pathway enrichment analysis,including neuroactive ligand-receptor interaction,retrograde endocannabinoid signaling and calcium signaling pathway.Docking results showed that the main constituents had certain affinities with the key targets.The active ingredients in CCEO regulated multiple signaling pathways to ameliorate pain through AR,ACHE,ESR1,GABRG2,PTGS2 and PPARγ.

Preventive effect of essential oil of Cinnamomum camphora leaves against mice allergic contact dermatitis

This article was aimed to study the antiinflammatory and antipruritic effects of essential oil of camphor leaves and its possible mechanism. The chemical constituents of essential oil of camphor leaves were analyzed by GC-MS. Mice allergic contact dermatitis (ACD) models were induced 0.5% 2,4-dinitrofluorobenzene (DNFB) solution. The contents of IL-4, 5-LOX, LTB4 and LTR1 in serum and the histomorphology of mice ear cutaneous tissue were measured after induction. Pathological examination showed a large number of inflammatory cells in the model group. Inflammatory cells were not seen in essential oil high dose group. The dermis slightly thickened in the low doses of essential oil group. The contents of 5-LOX, LTB4 and LTR1 of the drug groups were significantly decreased (P < 0.05) compared with that of the model group. However, the essential oil of camphor leaf had no significant effect on IL-4 (P > 0.05). Essential oil of camphor leaves has significant antiinflammatory and antipruritic effects on ACD by inhibiting the 5-LOX-LTB4-LTR1 pathway.

Simultaneous chromatographic fingerprinting and quantitative analysis of flavonoids in Pollen Typhae by high-performance capillary electrophoresis

To evaluate the quality of Pollen Typhae as used in traditional Chinese medicine,a high-performance capillary electrophoresis(HPCE)method has been developed,validated and applied to chromatographic fingerprinting and quantitation of its eight main bioactive flavonoids(naringenin,isorhamnetin 3-0-(2^(G)-α-L-rhamnosy)-rutinoside,rhamnetin 3-0-neohesperidoside,isorhamnetin,quercetin 3-0-(2^(G)-α-L-rhamnosyl)-rutinoside,quercetin 3-O-neohesperidoside,kaempferol and quer-cetin).Fingerprinting was based on the selection of nine characteristic chromatographic peaks.In quantitative analysis,the recovery of all eight compounds was in the range 98.5-102.2% with good linearity(r^(2)>0.9919)over a relatively wide concentration range.The assay was successfully applied to the analysis of the eight bioactive flavonoids in 14 different samples.The results indicate that the assay is reproducible and precise and can be used for convenient quality assessment of Pollen Typhae.