AIM: The localization of CB1 receptors in the spinalcord, spinal roots, dorsal root ganglion (DRG), andperipheral nerve of the rat was determined.METHODS: We studied the distribution of CB1cannabinoid receptors by imm...AIM: The localization of CB1 receptors in the spinalcord, spinal roots, dorsal root ganglion (DRG), andperipheral nerve of the rat was determined.METHODS: We studied the distribution of CB1cannabinoid receptors by immunohistochemistry usingan antibody raised against the N-termina1 of thereceptor. RESULTS: The spinal cord showednumerous transverse fibers labelled for CB1 receptorsthroughout and concentrated in the dorsal horn.Lightly-stained cells were observed throughout thespinal cord gray matter. The DRG also showed cellsand fibers labelled for CB1 receptors. Labelled fiberswere observed in both dorsal and ventral roots as well展开更多
AIM: To develop a sensitive method for measuring theputative endocannabinoid 2-arachidonylglycerol (2-AG)in the peripheral and ceatral nervous system.METHODS: A method using atmospheric pressurechemical ionization (AP...AIM: To develop a sensitive method for measuring theputative endocannabinoid 2-arachidonylglycerol (2-AG)in the peripheral and ceatral nervous system.METHODS: A method using atmospheric pressurechemical ionization (APCI) liquid chromatography/mass spectrometry (LC/MS ) was developed todetermine the levels of 2-AG in methanol extracts of therat lumar spinal cord, dorsal root ganglion (DRG),and sciatic nerve. RESULTS: 2-AG was detectedwith high sensitivity and minimal sample preparation.The levels in the tissues analyzed were ≤pmol/mg wetweight. Similar levels were found in the spinal展开更多
文摘AIM: The localization of CB1 receptors in the spinalcord, spinal roots, dorsal root ganglion (DRG), andperipheral nerve of the rat was determined.METHODS: We studied the distribution of CB1cannabinoid receptors by immunohistochemistry usingan antibody raised against the N-termina1 of thereceptor. RESULTS: The spinal cord showednumerous transverse fibers labelled for CB1 receptorsthroughout and concentrated in the dorsal horn.Lightly-stained cells were observed throughout thespinal cord gray matter. The DRG also showed cellsand fibers labelled for CB1 receptors. Labelled fiberswere observed in both dorsal and ventral roots as well
文摘AIM: To develop a sensitive method for measuring theputative endocannabinoid 2-arachidonylglycerol (2-AG)in the peripheral and ceatral nervous system.METHODS: A method using atmospheric pressurechemical ionization (APCI) liquid chromatography/mass spectrometry (LC/MS ) was developed todetermine the levels of 2-AG in methanol extracts of therat lumar spinal cord, dorsal root ganglion (DRG),and sciatic nerve. RESULTS: 2-AG was detectedwith high sensitivity and minimal sample preparation.The levels in the tissues analyzed were ≤pmol/mg wetweight. Similar levels were found in the spinal
