Direct choline administration in semi-intensive pisciculture system: A positive contaminant

Present work evaluated the possible impact of choline on metabolic enzymes of cultured fish species into a semi-intensive pond culture system,reared with Catla catla(catla),Labeo rohita(rahu),Clarias batracus(magur)and Anabas testudineus(climbing perch)for 90 days in two seasons.Choline was added directly into the pond water periodically throughout the experimental tenure.The water quality parameters of each experimental pond were monitored and were analyzed in 15 days interval throughout the experimental tenure in both the seasons.Results were compared with treatment as choline-fed and control as non-choline-fed conditions during breeding(June-Aug.)and dry(Nov.-Jan.)seasons.The metabolic enzymes,viz.,LDH(Lactate dehydrogenase),MDH(Malate dehydrogenase),and G6PDH(Glucose-6-phosphate dehydrogenase)activities in the muscle,liver,and AchE(Acetylcholine esterase)activity in the brain and muscle revealed maximum significantly(p<0.01),but ALT(Alanine aminotransferase)and AST(Aspartate aminotransferase)activity showed significant(p<0.01)depletion in the muscle and liver in treatment-breeding(TB)condition.The fishes under treatment-dry(TD)condition presented significant(p<0.01)higher elevation in HK(Hexokinase)activity in the muscle and liver.Additionally,the result of principal component analysis(PCA)depicted the positive as well as in some cases a negative correlation among the enzymatic activities both in dry and breeding seasons.So,it can be inferred that choline enrichment in fishes can substantiate the well nutritionally balanced food-flesh for consumption under this farm culture and the choline behaved as a positive contaminant into the pond eco-system under the semi-intensive culture condition.

Dose specific responses of Anabas testudineus(Bloch)to anthracene(PAH):Haematological and biochemical manifestation

Present work compared the toxicological responses of A.testudineus(Bloch)to anthracene exposure after treatment with two concentrations i.e.,25%(0.0075 mg/l)and 50%(0.015 mg/l)of LC50 value(0.030 mg/l)for different exposure periods of 1,5,10,15 and 21 day(s).An intensive comparison of the effects was made based on haematological and biochemical parameters of blood.Haematological responses revealed significant increase in some blood parameters,viz.,WBC,%LYM,MCV,MCH and MCHC but RBC,Hb,PCV and PLT showed decreasing trends.Biochemical responses like CHOLES,PRO,ALB,Trig showed enhanced pattern in the initial exposure of day one then reduced gradually but GLU,GPT,Ca,ALP,HDL,and LDL showed reverse pattern.Additionally,integrated biomarker responses(IBR)indicated that the biochemical parameters are more useful in determining the anthracene stress on fish health than the haematological parameters.Overall findings of this study suggested that the IBR analysis is a useful tool in monitoring quantitative(dose-specific)as well as qualitative(exposure-specific)responses to toxicological effects of anthracene exposure on fish health.