An endophytic fungus Aspergillus fumigatus,isolated from the fruits of Solanum insanum L.,was cultured in potato dextrose broth medium.Chromatographic separation of the EtOAc extract led to the isolation of six second...An endophytic fungus Aspergillus fumigatus,isolated from the fruits of Solanum insanum L.,was cultured in potato dextrose broth medium.Chromatographic separation of the EtOAc extract led to the isolation of six secondary metabolites,identified as pseurotin A(1),fumigaclavine C(2),monomethylsulochrin(3),trypacidin(4),fumiquinazoline C(5)and guignasulfide(6)by spectral means.Compounds 2 and 3 showed brine shrimp toxicity with IC50 values of 147 and 74.2μM,respectively,while compounds 4 and 5 showed slight inhibition on the growth of fungal pathogen Cladosporium cladosporioides.This is the first report of the isolation of guignasulfide(6)from A.fumigatus,which has been previously isolated only from an endophytic fungus Guignardia sp.展开更多
基金Financial assistance from the National Science Foundation of Sri Lanka(Grant No RG/2014/BS/02)is gratefully acknowledged.
文摘An endophytic fungus Aspergillus fumigatus,isolated from the fruits of Solanum insanum L.,was cultured in potato dextrose broth medium.Chromatographic separation of the EtOAc extract led to the isolation of six secondary metabolites,identified as pseurotin A(1),fumigaclavine C(2),monomethylsulochrin(3),trypacidin(4),fumiquinazoline C(5)and guignasulfide(6)by spectral means.Compounds 2 and 3 showed brine shrimp toxicity with IC50 values of 147 and 74.2μM,respectively,while compounds 4 and 5 showed slight inhibition on the growth of fungal pathogen Cladosporium cladosporioides.This is the first report of the isolation of guignasulfide(6)from A.fumigatus,which has been previously isolated only from an endophytic fungus Guignardia sp.
