Gut microbiome and serum metabolome analyses identify Bacteroides fragilis as regulators of serotonin content and PRL secretion in broody geese

Broody behavior is regulated by hypothalamic prolactin secretion,which seriously affects egg production in poulty production.Numerous studies have provided evidence that animal behavior is governed by dynamic bidirectional communication between specific gut bacteria and their host via the brain-gut-microbiome axis.However,little research focused on how the gut microbiota influence broody behavior in poultry.In this study,Zhedong white geese in laying and brooding phases were selected.Ten differentially abundant bacteria in cecum were detected between brooding and laying geese through metagenomic analyses and 16S rRNA sequencing(P<0.05),and Bacteroides fragilis was specifically identified as a key driver species in the brooding geese.Moverover,the serum metabolites were quantified,and the 313 differentially abundant metabolites were found between the two groups of different physiological geese.They were primarily enriched in the tryptophan metabolism pathways.Pearson correlation analyses revealed there was a significant positive correlation between B.fragilis abundance and the context of 11 tryptophan metabolism-related metabolites(such as serotonin,etc.)in broody geese,which hinted that those tryptophan metabolites might be produced or driven by B.fragilis.Finally,the serum hormone levels were also measured.We found there was a positive correlation between B.fragilis abundance and content of serotonin.Besides,prolactin secreted by the pituitary gland was greater in brooding geese than that in laying geese,which was also highly correlated with B.fragilis abundance.This result implied that B.fragilis could promote the secretion of prolactin by the pituitary gland.Together,the current study findings provided the information on gut microbiota influencing broody behavior,B.fragilis produced or driven more serum serotonin,and stimulated the pituitary gland to secret more prolactin,which potentially offered a new enlightenment for the intervention of broody behavior in poultry.

Modeling load distribution for rural photovoltaic grid areas using image recognition

Expanding photovoltaic(PV)resources in rural-grid areas is an essential means to augment the share of solar energy in the energy landscape,aligning with the“carbon peaking and carbon neutrality”objectives.However,rural power grids often lack digitalization;thus,the load distribution within these areas is not fully known.This hinders the calculation of the available PV capacity and deduction of node voltages.This study proposes a load-distribution modeling approach based on remote-sensing image recognition in pursuit of a scientific framework for developing distributed PV resources in rural grid areas.First,houses in remote-sensing images are accurately recognized using deep-learning techniques based on the YOLOv5 model.The distribution of the houses is then used to estimate the load distribution in the grid area.Next,equally spaced and clustered distribution models are used to adaptively determine the location of the nodes and load power in the distribution lines.Finally,by calculating the connectivity matrix of the nodes,a minimum spanning tree is extracted,the topology of the network is constructed,and the node parameters of the load-distribution model are calculated.The proposed scheme is implemented in a software package and its efficacy is demonstrated by analyzing typical remote-sensing images of rural grid areas.The results underscore the ability of the proposed approach to effectively discern the distribution-line structure and compute the node parameters,thereby offering vital support for determining PV access capability.

The Chinese guideline for management of snakebites

In 2009,the World Health Organization included snakebite on the list of neglected tropical diseases,acknowledging it as a common occupational hazard for farmers,plantation workers,and others,causing tens of thousands of deaths and chronic physical disabilities every year.This guideline aims to provide practical information to help clinical professionals evaluate and treat snakebite victims.These recommendations are based on clinical experience and clinical research evidence.This guideline focuses on the following topics:snake venom,clinical manifestations,auxiliary examination,diagnosis,treatments,and prevention.

Quantifying the Temperature Effects on the Morbidity of Digestive Diseases In an Inland Plateau-Valley City

Extreme air temperature and increased weather oscillations caused by climate change have been threatening global health.Meteorological conditions are external inducers that may trigger the onset of gastrointestinal diseases,in addition to bacterial infection or behavioral factors including smoking,alcohol consumption,and hot food consumption[1,2].

Forced Inspiratory Flow Volume Curve in Patients with Obstructive Sleep Apnea-Hypopnea Syndrome

Objective: Obstructive sleep apnea-hypopnea syndrome (OSA) is a disease of obstructive apnea or hypopnea caused by a repeated partial or complete collapse of the upper airway during sleep. The inspiratory part of the flow-volume curve (F-V curve) can be used as an auxiliary means to evaluate upper airway obstruction in adults. This study is to evaluate the ability of the F-V curve to predict the OSA and explore inspiratory indicators related to airway obstruction during sleep. Methods: There were 332 patients included in this cross-sectional study, who were accompanied by snoring, daytime sleepiness and other symptoms, with suspicion of OSA. According to the nocturnal polysomnography, the subjects were distributed into mild to moderate OSA group, severe OSA group and non-OSA group. A pulmonary function test was used to collect the subjects’ spirometry and F-V curves. Results: There was no significant difference in a variety of indices derived from the F-V curve between OSA and normal subjects, including 25% inspiratory flow rate, middle inspiratory flow rate, 75% inspiratory flow rate, peak flow rate, and forced inspiratory flow rate in the first second. The pulmonary function parameters were significantly correlated with the weight, age and sex of the subjects. Conclusion: These findings suggest that the inspiratory curve of pulmonary function cannot evaluate the upper airway abnormalities in patients with obstructive apnea-hypopnea syndrome.

SYNTHESIS AND CHARACTERIZATION OF SILAZANE OLIGOMER CONTAINING 1,1,3,3,5,5-HEXAMETHYLCYCLOTRISILAZANE

A new silazane oligomer containing cyclotrisilazane was synthesized by KH-catalyzed dehydro-coupling reactions between diphenylsilane and 1,1,3,3,5,5-hexamethylcyclotrisilazane (D-3(N)).

Synthesis of a New Polysiloxane Containing Octaphenylcyclodisilazane

A new polysiloxane containing octaphenylctclodisilazane was synthesized from the anionic polymerization of octamethylcyclotetrasiloxane (D-4) and 'seed solution' as initiator. which was prepared by hexamethylcyclotrisilozane (D-3) and the lithium salt of N,N'-bis(hydroxydiphenyl)tetraphenylcyclodisilazane.

The effective role of CsTCP5 and CsTCP18 transcription factors from Camellia sinensis(L.)O.Kuntze under drought and low-temperature

TCP transcription factors are essential for plant growth and environmental response,but little is known about their specific roles in growth,hormone response,and stress response in tea plants.Here,CsTCP5 and CsTCP18 were cloned from the tea variety'Longjing 43',and subcellular localization revealed that both CsTCP5 and CsTCP18 proteins were present in the nucleus.Exogenous abscisic acid(ABA)and methyl jasmonate(MeJA)had different effects on CsTCP5 and CsTCP18 expression,but both genes were repressed by drought and low-temperature treatment.Yeast-two hybrid(Y2H)and bimolecular fluorescence complementation(BiFC)assays demonstrated that neither transcription factor was transcriptionally active but that each interacted with members of the CsMYB and CsJAZ families.Transgenic Arabidopsis plants overexpressing CsTCP5 had smaller cotyledons and fewer lateral roots compared with wild-type(WT)and empty vector(EV)Arabidopsis,and their root growth and germination rate were inhibited by ABA and MeJA treatment.Lateral root numbers also decreased significantly in CsTCP18-OE Arabidopsis after MeJA treatment.These results demonstrate that CsTCP5 and CsTCP18 have regulatory effects on cotyledon development,lateral root growth,and germination rate,and these effects can be modulated by ABA and MeJA.Under drought stress,the CsTCP5-OE and CsTCP18-OE lines exhibited greater survival,lower plant height,smaller rosette leaves,delayed flowering,increased activities of antioxidant enzymes,decreased MDA content,and increased proline content compared with WT and EV Arabidopsis.These findings suggest that CsTCP5 and CsTCP18 are important for tea plant growth,interact with ABA and MeJA pathways,and play roles in stress response.

Measurement of solar pp neutrino flux using electron recoil data from PandaX-4T commissioning run

The proton-proton(pp)fusion chain dominates the neutrino production in the Sun.The uncertainty of the predicted pp neutrino flux is at the sub-percent level,whereas that of the best measurement is O(10%).In this study,for the first time,we measure solar pp neutrinos in the electron recoil energy range from 24 to 144 keV using the PandaX-4T commissioning data with 0.63 tonne×year exposure.The pp neutrino flux is determined as(8.0±3.9(stat)±10.0(syst))×1010 s^(-1)cm^(-2),which is consistent with the Standard Solar Model and existing measurements,corresponding to an upper flux limit of 23.3×10^(10)s^(-1)cm^(-2)at 90%C.L..

High frequency of thrombocytopenia in patients with acute-on-chronic liver failure treated with linezolid

BACKGROUND： Linezolid is an effective antibiotic reagent for Gram-positive bacterial infection; its most common side effect is thrombocytopenia. However, the incidence of throm- bocytopenia in patients with acute-on-chronic liver failure （ACLF） who underwent linezolid therapy was unclear. The present study was to evaluate the incidence of thrombocyto- penia in ACLF and non-ACLF patients treated with linezolid and the risk factors of thrombocytopenia in these patients.

HBsAg levels in HBeAg-positive chronic hepatitis B patients with different immune conditions

AIM: To investigate hepatitis B surface antigen (HBsAg) levels in patients with HBeAg-positive chronic hepatitis B (CHB) and different immune conditions.

Attitude Control of Multiple Rigid Bodies with Uncertainties and Disturbances

Decentralized attitude synchronization and tracking control for multiple rigid bodies are investigated in this paper. In the presence of inertia uncertainties and environmental disturbances, we propose a class of decentralized adaptive sliding mode control laws. An adaptive control strategy is adopted to reject the uncertainties and disturbances. Using the Lyapunov approach and graph theory, it is shown that the control laws can guarantee a group of rigid bodies to track the desired time-varying attitude and angular velocity while maintaining attitude synchronization with other rigid bodies in the formation. Simulation examples are provided to illustrate the feasibility and advantage of the control algorithm. © 2014 Chinese Association of Automation.

Step-by-Step Numerical Prediction of Aerodynamic Noise Generated by High Speed Trains

In this paper,the unsteady flow around a high-speed train is numerically simulated by detached eddy simulation method(DES),and the far-field noise is predicted using the Ffowcs Williams-Hawkings(FW-H)acoustic model.The reliability of the numerical calculation is verified by wind tunnel experiments.The superposition relationship between the far-field radiated noise of the local aerodynamic noise sources of the high-speed train and the whole noise source is analyzed.Since the aerodynamic noise of high-speed trains is derived from its different components,a stepwise calculation method is proposed to predict the aerodynamic noise of high-speed trains.The results show that the local noise sources of high-speed trains and the whole noise source conform to the principle of sound source energy superposition.Using the head,middle and tail cars of the high-speed train as noise sources,different numerical models are established to obtain the far-field radiated noise of each aerodynamic noise source.The far-field total noise of high-speed trains is predicted using sound source superposition.A step-by-step calculation of each local aerodynamic noise source is used to obtain the superimposed value of the far-field noise.This is consistent with the far-field noise of the whole train model’s aerodynamic noise.The averaged sound pressure level of the far-field longitudinal noise measurement points differs by 1.92 dBA.The step-by-step numerical prediction method of aerodynamic noise of high-speed trains can provide a reference for the numerical prediction of aerodynamic noise generated by long marshalling high-speed trains.

Numerical simulation and optimization of aerodynamic uplift force of a high-speed pantograph

Aiming at the problem that aerodynamic uplift forces of the pantograph running in the knuckle-downstream and knuckle-upstream conditions are inconsistent,and their magnitudes do not satisfy the corresponding standard, the aerodynamic uplift forces of pantographs with baffles are numerically investigated, and an optimization method to determine the baffle angle is proposed. First, the error between the aerodynamic resistances of the pantograph obtained by numerical simulation and wind tunnel test is less than 5%, which indicates the accuracy of the numerical simulation method. Second, the original pantograph and pantographs equipped with three different baffles are numerically simulated to obtain the aerodynamic forces and moments of the pantograph components.Three different angles for the baffles are-17°, 0° and 17°.Then the multibody simulation is used to calculate the aerodynamic uplift force of the pantograph, and the optimal range for the baffle angle is determined. Results show that the lift force of the baffle increases with the increment of the angle in the knuckle-downstream condition, whereas the lift force of the baffle decreases with the increment of the angle in the knuckle-upstream condition. According to the results of the aerodynamic uplift force, the optimal angle of the baffle is determined to be 4.75° when the running speed is 350 km/h, and pantograph–catenary contact forces are 128.89 N and 129.15 N under the knuckledownstream and knuckle-upstream operating conditions,respectively, which are almost equal and both meet the requirements of the standard EN50367:2012.

Erratum:A Search for Solar Axions and Anomalous Neutrino Magnetic Moment with the Complete PandaX-Ⅱ Data[CHIN.PHYS.LETT.38(2021)011301]

In our published letter,[1]we have identified a minor error in Figs.2 and 3.Instead,we have 2111 events in these two plots with 646,249,382,and 834 events in Run 9(20.0 ton·day),Run 10(19.4 ton·day),Run 11–1(24.2 ton·day),and Run 11–2(37.1 ton·day).The mistake is due to an incorrect application of a small energy non-linearity(known as the BLS non-linearity)in making plots,but has no impact to the final results.Figures 2 and 3 are now updated in this note.

Ureido-modification of the resin-bound LHRH analogue with N,N'-carbonyldiimidazole

The ureido-modification of the resin-bound a luteinizing hormone releasing hormone （LHRH） analogue was investigated by CDI-activating method. The amino group at the side chain of LHRH analogue could be transformed into various substituted urea moieties in high yields. However, its terminal amino group was partially converted to a hydantoin structure due to the attack of the N atom of the adjacent amide bond.

The Growth Path of Agricultural Labor Productivity in Major Grain Producing Areas

The growth of agricultural labor productivity in major grain producing areas arises from the increase in production factors or improvement of efficiency,and is related to the sustainability of agricultural economic growth and national food security. We study the growth path of agricultural labor productivity in 13 major grain producing areas of China,and the analysis results show that from the relative endowments of land and labor in major grain producing areas,the growth rate of the output value per unit of labor is higher than that of the productivity per unit of land; in essence,this growth path of increasing the output value relying on increasing the production factor inputs is the reduction of labor,and the reduction of agricultural labor has a great elasticity of impact on the total value of agricultural production. In the case of stable future number of agricultural labor in China,the growth of agricultural labor productivity will be impossible to rely more on the increase in agricultural production factor inputs,and the growth path of agricultural economy still has to rely on the improvement of land productivity and take the efficiency-based path.

Formation of composite hydrogel of carboxymethyl konjac glucomannan/gelatin for sustained release of EGCG

Development of functional bioinspired hydrogels that have good releases control character is necessary for the application of these materials in biomedical engineering.Herein,we report a composite hydrogel prepared from several biocompatible carboxymethyl konjac glucomannan(CKGM)/gelatin(G)/tannic acid(TA)functional nano-hydroxyapatite(TA@n-HA),which has good biodegradability and pH sensitivity.The mechanism of interaction between hydrogels was confirmed by Fourier transform infrared spectroscopy,X-ray diffraction,Scanning electron microscopy and Thermogravimetric analysis.The physico-chemical properties of CKGM/G hydrogels have been significantly improved through the incorporation of TA@n-HA within the matrix.Studies in the sustained release of epigallocatechin gallate(EGCG)demonstrated that the TA@n-HA/CKGM/G hydrogels exhibit not only better pH sensitive properties,but also enhanced biocompatibility and encapsulation in comparison to the matrix devoid of TA@n-HA.Consequently,TA@n-HA/CKGM/G hydrogels using EGCG as a drug release model show the potential for drug delivery.

Expert consensus on emergency medicine procedure optimization guided by routine prevention and control strategy for COVID-19

The outbreak of coronavirus disease 2019(COVID-19)was declared a global public health emergency on 31 January 2020.Emergency medicine procedures in Emergency Department should be optimized to cope with the current COVID-19 pandemic by providing subspecialty services,reducing the spread of nosocomial infections,and promoting its capabilities to handle emerging diseases.Thus,the Chinese Society of Emergency Medicine and Wuhan Society of Emergency Medicine drafted this consensus together to address concerns of medical staffs who work in Emergency Department.Based on in-depth review of COVID-19 diagnosis and treatment plans,literatures,as well as management approval,this consensus proposes recommendations for improving the rationalization and efficiency of emergency processes,reducing the risk of nosocomial infections,preventing hospital viral transmission,and ensuring patient safety.

A Facile Synthesis Route to 1,3-dichlorodisilazanes

A new 1,3-dichlorodisilazane, 1,3-dichloro-1,3-dimethyl-1,3-divinyldisilazane (DMDV), was prepared from the reaction of hexamethyldisilazane (MMN) and methylvinyldichlorosilane. And use the same method, 1,3-dichloro-1,1,3,3-tetraphenyldisilazane (DCTPS) was also obtained in a high yield.