Human induced pluripotent stem cells(hiPSCs)are invaluable resources for producing high-quality differentiated cells in unlimited quantities for both basic research and clinical use.They are particularly useful for st...Human induced pluripotent stem cells(hiPSCs)are invaluable resources for producing high-quality differentiated cells in unlimited quantities for both basic research and clinical use.They are particularly useful for studying human disease mechanisms in vitro by making it possible to circumvent the ethical issues of human embryonic stem cell research.However,significant limitations exist when using conventional flat culturing methods especially concerning cell expansion,differentiation efficiency,stability maintenance and multicellular 3D structure establishment,differentiation prediction.Embryoid bodies(EBs),the multicellular aggregates spontaneously generated from iPSCs in the suspension system,might help to address these issues.Due to the unique microenvironment and cell communication in EB structure that a 2D culture system cannot achieve,EBs have been widely applied in hiPSC-derived differentiation and show significant advantages especially in scaling up culturing,differentiation efficiency enhancement,ex vivo simulation,and organoid establishment.EBs can potentially also be used in early prediction of iPSC differentiation capability.To improve the stability and feasibility of EB-mediated differentiation and generate high quality EBs,critical factors including iPSC pluripotency maintenance,generation of uniform morphology using micro-pattern 3D culture systems,proper cellular density inoculation,and EB size control are discussed on the basis of both published data and our own laboratory experiences.Collectively,the production of a large quantity of homogeneous EBs with high quality is important for the stability and feasibility of many PSCs related studies.展开更多
To the Editor：A 43-year-old woman presented with mulberry-like plaques on the left side of her back.At the same site,she had acquired herpes zoster approximately 3 months ago and been healed after treatments.The cond...To the Editor：A 43-year-old woman presented with mulberry-like plaques on the left side of her back.At the same site,she had acquired herpes zoster approximately 3 months ago and been healed after treatments.The condition started as small plaques and pimples in irregular shapes,followed by the extension of the new lesions with acupuncture-like pain and tenderness upon palpation.展开更多
Introduction In 1991,Anhalt et al.[1] finst described a rare form of atypical pemphigus that was associated with lymphoproliferative disease.In 1999,Zhu et al.[2] reported the first case of paraneoplastic pemphigus (P...Introduction In 1991,Anhalt et al.[1] finst described a rare form of atypical pemphigus that was associated with lymphoproliferative disease.In 1999,Zhu et al.[2] reported the first case of paraneoplastic pemphigus (PNP) in China and then demonstrated the mechanism by which reactive autoantibodies in PNP were produced by the cells of associated tumors.展开更多
基金Supported by National Natural Science Foundation of China,No.81770621,No.81573053Ministry of Education,Culture,Sports,Science,and Technology of Japan,KAKENHI,No.16K15604,No.18H02866Natural Science Foundation of Jiangsu Province,No.BK20180281
文摘Human induced pluripotent stem cells(hiPSCs)are invaluable resources for producing high-quality differentiated cells in unlimited quantities for both basic research and clinical use.They are particularly useful for studying human disease mechanisms in vitro by making it possible to circumvent the ethical issues of human embryonic stem cell research.However,significant limitations exist when using conventional flat culturing methods especially concerning cell expansion,differentiation efficiency,stability maintenance and multicellular 3D structure establishment,differentiation prediction.Embryoid bodies(EBs),the multicellular aggregates spontaneously generated from iPSCs in the suspension system,might help to address these issues.Due to the unique microenvironment and cell communication in EB structure that a 2D culture system cannot achieve,EBs have been widely applied in hiPSC-derived differentiation and show significant advantages especially in scaling up culturing,differentiation efficiency enhancement,ex vivo simulation,and organoid establishment.EBs can potentially also be used in early prediction of iPSC differentiation capability.To improve the stability and feasibility of EB-mediated differentiation and generate high quality EBs,critical factors including iPSC pluripotency maintenance,generation of uniform morphology using micro-pattern 3D culture systems,proper cellular density inoculation,and EB size control are discussed on the basis of both published data and our own laboratory experiences.Collectively,the production of a large quantity of homogeneous EBs with high quality is important for the stability and feasibility of many PSCs related studies.
基金This study was＇supported by grants from the National Natural Science Foundation （No. 81573053）, the Maternal and Child Health Project of Jiangsu Province （No. F201717）, and the Science and Technology Project in Zhenjiang （No. SS2015024）.
文摘To the Editor：A 43-year-old woman presented with mulberry-like plaques on the left side of her back.At the same site,she had acquired herpes zoster approximately 3 months ago and been healed after treatments.The condition started as small plaques and pimples in irregular shapes,followed by the extension of the new lesions with acupuncture-like pain and tenderness upon palpation.
基金the National Natural Science Foundation of China(No.81573053)the Training Funding Project('333 Project')of Jiangsu Province(No.BRA2015196)the Seventh Batch of Science and Technology Plan in Zhenjiang(No.SS2015024).
文摘Introduction In 1991,Anhalt et al.[1] finst described a rare form of atypical pemphigus that was associated with lymphoproliferative disease.In 1999,Zhu et al.[2] reported the first case of paraneoplastic pemphigus (PNP) in China and then demonstrated the mechanism by which reactive autoantibodies in PNP were produced by the cells of associated tumors.
