A novel kinetic method for determination of uric acid in human serum by means of an uncatalyzed BZ system consisting of potassium bromate and p-hydroxybenzaldehyde （p-HBA） in sulfuric acid medium was proposed, in wh...A novel kinetic method for determination of uric acid in human serum by means of an uncatalyzed BZ system consisting of potassium bromate and p-hydroxybenzaldehyde （p-HBA） in sulfuric acid medium was proposed, in which the analyte perturbation to the system was recorded close to the bifurcation point. The potential change was directly proportional to the logarithm of concentration of uric acid in the range of 3.73 × 10 8--7.48 × 10-4 mol.L-1 （r=0.9983） with a detection limit of 7.45 × 10-9mol.L-I and a recovery from 98.9% to 101.1%. A comparison between the proposed technique and other methods indicated that results obtained were in agreement with those in clinical detection. In addition, the possible mechanism of action of uric acid on the uncatalyzed BZ reaction was also discussed briefly.展开更多
基金This work was supported in part by the Project of International Cooperation between China and Ukraine （No. 043-05）, the National Natural Science Foundation of China （No. 20873101）, the Natural Science Foundation of Gansu （No. 1010RJZA015） and the project LKQN-08-9 of Northwest Normal University, China.
文摘A novel kinetic method for determination of uric acid in human serum by means of an uncatalyzed BZ system consisting of potassium bromate and p-hydroxybenzaldehyde （p-HBA） in sulfuric acid medium was proposed, in which the analyte perturbation to the system was recorded close to the bifurcation point. The potential change was directly proportional to the logarithm of concentration of uric acid in the range of 3.73 × 10 8--7.48 × 10-4 mol.L-1 （r=0.9983） with a detection limit of 7.45 × 10-9mol.L-I and a recovery from 98.9% to 101.1%. A comparison between the proposed technique and other methods indicated that results obtained were in agreement with those in clinical detection. In addition, the possible mechanism of action of uric acid on the uncatalyzed BZ reaction was also discussed briefly.
