Mycoplasma capricolum subsp.capricolum(Mcc)is an important etiological agent of contagious agalactia(CA).CA affects small ruminants and is characterized by inducing mastitis,arthritis,kerato-conjunctivitis and respira...Mycoplasma capricolum subsp.capricolum(Mcc)is an important etiological agent of contagious agalactia(CA).CA affects small ruminants and is characterized by inducing mastitis,arthritis,kerato-conjunctivitis and respiratory symptoms.The aim of this study was to isolate and characterize Mcc from Moroccan goats with contagious agalactia.A total of 300 Alpine goats were monitored.Serology analysis,molecular identification,and isolation of Mcc were realized from suspected goats.An experimental study was conducted for isolated Mcc to determine their pathogenicity.Thus,clinical observation showed that respiratory symptoms were predominant in young animals,and other symptoms,such as mastitis,keratoconjunctivitis and lameness,were more frequently detected in adult goats.Of the 80 tested blood samples,28 sera were seropositive for Mcc antibodies.Mcc was identified by polymerase chain reaction(PCR)in milk,lung tissue and synovial liquid samples.The isolation of Mcc was successful through bacterial culture from lung tissue.LppA gene sequence of this strain revealed 98.1%similarity with the reference strain(ATCC 27343),with 11 missense variants.Experimental infection resulted in severe and generalized CA disease in sheep and goats,confirming the high pathogenicity of the Moroccan Mcc isolate.展开更多
In this study,55 suspected pasteurellosis clinical cases from different provinces of Morocco were investigated.Molecular analysis revealed that 47%of samples were positive for Pasteurella multocida,all typed as serogr...In this study,55 suspected pasteurellosis clinical cases from different provinces of Morocco were investigated.Molecular analysis revealed that 47%of samples were positive for Pasteurella multocida,all typed as serogroup A,and 11%positive for Mannheimia heamolytica.Eight isolates were recovered from 26 P.multocida positive samples,and characterized by biochemical and molecular typing methods.Among these isolates,two strains(S13 and S14)were selected for genes(RNA16S and rpoB)sequence analysis and virulence study in mice,guinea pigs and sheep.Phylogeny study showed similarities of both S14 and S13 isolates with strains from other species.In laboratory animals,the strain S14 was more virulent than S13 and induced severe illness in sheep.The high mortality of infected mice suggests that this model may represent an alternative for testing pathogenicity and vaccine efficacy.展开更多
文摘Mycoplasma capricolum subsp.capricolum(Mcc)is an important etiological agent of contagious agalactia(CA).CA affects small ruminants and is characterized by inducing mastitis,arthritis,kerato-conjunctivitis and respiratory symptoms.The aim of this study was to isolate and characterize Mcc from Moroccan goats with contagious agalactia.A total of 300 Alpine goats were monitored.Serology analysis,molecular identification,and isolation of Mcc were realized from suspected goats.An experimental study was conducted for isolated Mcc to determine their pathogenicity.Thus,clinical observation showed that respiratory symptoms were predominant in young animals,and other symptoms,such as mastitis,keratoconjunctivitis and lameness,were more frequently detected in adult goats.Of the 80 tested blood samples,28 sera were seropositive for Mcc antibodies.Mcc was identified by polymerase chain reaction(PCR)in milk,lung tissue and synovial liquid samples.The isolation of Mcc was successful through bacterial culture from lung tissue.LppA gene sequence of this strain revealed 98.1%similarity with the reference strain(ATCC 27343),with 11 missense variants.Experimental infection resulted in severe and generalized CA disease in sheep and goats,confirming the high pathogenicity of the Moroccan Mcc isolate.
文摘In this study,55 suspected pasteurellosis clinical cases from different provinces of Morocco were investigated.Molecular analysis revealed that 47%of samples were positive for Pasteurella multocida,all typed as serogroup A,and 11%positive for Mannheimia heamolytica.Eight isolates were recovered from 26 P.multocida positive samples,and characterized by biochemical and molecular typing methods.Among these isolates,two strains(S13 and S14)were selected for genes(RNA16S and rpoB)sequence analysis and virulence study in mice,guinea pigs and sheep.Phylogeny study showed similarities of both S14 and S13 isolates with strains from other species.In laboratory animals,the strain S14 was more virulent than S13 and induced severe illness in sheep.The high mortality of infected mice suggests that this model may represent an alternative for testing pathogenicity and vaccine efficacy.
