In situ biosynthesized silver nanoparticle-incorporated synthesized zeolite A using Orthosiphon aristatus extract for in vitro antibacterial wound healing

The capability of synthesized zeolite A(SZ)to immobilize Ag ions(Ag-SZ)and Ag nanoparticles(AgNp-SZ)were comparatively studied.A novel approach of in situ biosynthesized AgNP-incorporated synthesized zeolite A(AgNp-SZ)was synthesized at an optimum volume of 0.4mL of the Orthosiphon aristatus(O.aristatus)leaves plant extract(5%)using an in situ approach.In comparison,Ag-Sz was produced by loading the synthesized zeolite with Ag ions.All synthesized materials were characterized for their morphologies and physicochemicalproperties.The characterization analysesvalidate thathe biosynthesized AgNP(<100 nm)using O.aristatus leaves extract was incorporated into the zeolite A.The antibacterial testing confirmed that these materials have antibacterial activity against Escherichia coliATCC11229 and Staphylococcus aureus ATCC 6538.MIC/MBC analysis demonstrated that in 0.9%saline solution,AgNP-SZ had higher antibacterial activity than Ag-Sz.The in vitro cell viability and migration assays were further examined towards human skin fibroblast cells HSF 1184.Results show that the materials are not cytotoxicto HSF1184,and the biosynthesized AgNP-SZ promotes cell migration and proliferation higher than Ag-SZ.This research proved that the biocompatible antibacterial wound healing agent of AgNP-SZ can be synthesized using an in situ approach where the reduction process of Ag ions in the zeolite Acan be performed using plant extract.

Antibacterial, cytotoxicity and wound healing assessments of amine-functionalised zeolite Y

The antibacterial activities,cytotoxicity,and wound healing of amine(3-aminopropyltriethoxysilane (APTES))-functionalised zeolite Y against normal human fibroblast cells were studied.The characterisation of unmodified and amine-functionalised zeolites Y (Z,ZA 0.04,ZA 0.4,and ZA 0.6) by Fourier transform infrared spectroscopy,X-ray powder diffraction,and energy dispersive X-ray analysis proved that the APTES molecules were grafted onto the zeolite Y surface without distorting its framework structure.All amine-functionalised zeolite Y samples possessed antibacterial activities against several ATCC bacteria that were correlated with the increased amount of APTES on the zeolite Y surface.Conversely,when a higher concentration of APTES was grafted on the zeolite Y,higher cytotoxicity was observed against the fibroblast cells.Although the ZA 0.6 sample (zeolite Y functionalised with 0.6 M of APTES) had higher antibacterial activity,it was cytotoxic to the cells.Therefore,the selection of an antibacterial agent for human treatment purposes must also consider its cytotoxicity effect against human cells to ensure it is biocompatible.

Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: Antibacterial, cytocompatibility, and in vitro wound healing evaluation

Biogenic synthesis of silver nanoparticles(b-AgNPs)utilising plant extract has gained the interest of researchers due to the environmentally friendly and cost-effective technique.However,the extent of its application in the biomedical field remains scarce.This study evaluates the antibacterial,cytocompatibility,and wound healing activities of synthesised AgNPs using Persicaria odorata leaves extract(PO-AgNPs).The formation of PO-AgNPs was observed by visual colour changes and verified by ultraviolet-visible(UV–vis)spectrophotometer,which revealed a surface plasmon resonance(SPR)at around 440 nm,and further confirmed by X-ray diffraction(XRD).Characterisation using Fourier transform infrared(FTIR)spectroscopy showed biomolecules from the leaves extract presented together on PO-AgNPs.Field emission scanning electron microscope(FESEM)and high-resolution transmission electron microscopy(HR-TEM)images revealed PO-AgNPs nanospheres with diameters of 11±3 nm.Disc diffusion test(DDT)and minimum inhibitory concentration(MIC)analysis resulted in a dose-dependent inhibition of PO-AgNPs against tested Staphylococcus epidermidis and Methicillin-resistant Staphylococcus aureus(MRSA).These results were further corroborated by time-kill kinetic assay which revealed that PO-AgNPs were bactericidal against both strains 24 h post-treatment.Cytocompatibility and in vitro wound healing evaluation against normal human fibroblast cells,HSF 1184 inferred that PO-AgNPs are non-toxic to normal cells and able to enhance cell migration as compared to the non-treated cells.Therefore,PO-AgNPs are biocompatible and possess antibacterial and wound healing capabilities that are useful in biomedical applications.