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白木香AsMAPK3基因的原核表达及其在洋葱表皮瞬时表达体系中的定位研究
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作者 徐艳红 田美慧 +2 位作者 吕菲菲 孙佩文 魏建和 《中草药》 CAS CSCD 北大核心 2018年第9期2133-2139,共7页
目的构建国产沉香基原植物白木香AsMAPK3基因的原核表达载体,诱导重组蛋白正确表达,并研究AsMAPK3的亚细胞定位情况,为抗体制备准备材料,为筛选其互作蛋白及进一步研究其功能奠定基础。方法通过PCR方法克隆AsMAPK3基因的部分编码序列,... 目的构建国产沉香基原植物白木香AsMAPK3基因的原核表达载体,诱导重组蛋白正确表达,并研究AsMAPK3的亚细胞定位情况,为抗体制备准备材料,为筛选其互作蛋白及进一步研究其功能奠定基础。方法通过PCR方法克隆AsMAPK3基因的部分编码序列,构建重组原核表达载体pET-28a-AsMAPK3,并诱导蛋白表达。克隆AsMAPK3基因全长编码区,构建绿色荧光蛋白(GFP)瞬时表达载体pAN580-AsMAPK3,通过基因枪法转化洋葱表皮,荧光显微镜观察GFP的发光情况。结果含有重组表达载体的大肠杆菌BL21(DE3)在37℃经0.5 mmol/L异丙基硫代β-D-半乳糖苷(IPTG)诱导4 h后,可获得相对分子质量约为39 000的融合蛋白,该融合蛋白在上清和包涵体中均有表达。瞬时转化洋葱表皮的GFP荧光观察发现,AsMAPK3主要在细胞核和质膜表达。结论成功实现了AsMAPK3的体外表达和纯化,明确了AsMAPK3的亚细胞定位,为后续开展其功能研究奠定了基础。 展开更多
关键词 白木香 MAPK 原核表达 瞬时表达 大肠杆菌
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Quantifying soil erosion with GIS-based RUSLE under different forest management options in Jianchang Forest Farm 被引量:3
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作者 ZHANG Hengming, WANG Qingli, DAI Limin, Guofan Shao, TANG Una, WANG Shunzhong & GU Huiyan Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, china Graduate university of Chinese Academy of Sciences, Beijing 100101, china +1 位作者 Department of forestry and Natural Resources, Purdue university, Lafayette IN47901, USA northeast forestry university, harbin 150040, china 《Science China(Technological Sciences)》 SCIE EI CAS 2006年第z1期160-166,共7页
Quantitatively estimating soil erosion with an integration of geographic information system (GIS) and the revised universal soil loss equation (RUSLE) under four different exposed soil proportion scenarios caused by f... Quantitatively estimating soil erosion with an integration of geographic information system (GIS) and the revised universal soil loss equation (RUSLE) under four different exposed soil proportion scenarios caused by forest management practices was studied at Jianchang Forest Farm. The GIS provided means of input data generation required by RUSLE model and allowed a spatial assessment of the erosion hazard over the study area. Four exposed soil proportion scenarios of 5%, 10%, 20% and 30% were tested with the GIS-based RUSLE model to evaluate soil erosion hazard. The predicted soil erosion potentials were classified into five categories in order to provide valuable aids for management planning. 展开更多
关键词 GEOGRAPHIC information system RUSLE soil erosion forest management.
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