Significant advancements in various research and technological fields have contributed to remarkable findings on the physiological dynamics of the human body.Tomore closely mimic the complex physiological environment,...Significant advancements in various research and technological fields have contributed to remarkable findings on the physiological dynamics of the human body.Tomore closely mimic the complex physiological environment,research has moved from two-dimensional(2D)culture systems to more sophisticated three-dimensional(3D)dynamic cultures.Unlike bioreactors or microfluidic-based culture models,cells are typically seeded on polymeric substrates or incorporated into 3D constructs which are mechanically stimulated to investigate cell response to mechanical stresses,such as tensile or compressive.This review focuses on the working principles of mechanical stimulation devices currently available on the market or custom-built by research groups or protected by patents and highlights the main features still open to improvement.These are the features which could be focused on to perform,in the future,more reliable and accurate mechanobiology studies.展开更多
Laser surface texturing is a versatile approach for manufacturing implants with suitable surfaces for os-seointegration.This work explores the use of laser to fabricate NiTi textured implants,testing two dif-ferent gr...Laser surface texturing is a versatile approach for manufacturing implants with suitable surfaces for os-seointegration.This work explores the use of laser to fabricate NiTi textured implants,testing two dif-ferent groove-based designs.Their performance was evaluated in vivo through implantation in Sprague Dawley rats’femur,being then analyzed after 4 and 12 weeks of implantation.Push-out experiments and histological characterization allowed to assess bone-implant bond and osseointegration and to compare the laser textured solutions with non-textured NiTi.Histology showed that,at 4 weeks of implantation,mainly immature woven bone was present whilst at 12 weeks a more mature bone had developed.Con-sidering the largest implantation time(12 weeks),results showed extraction forces considerably higher for textured implants(G2 and G3).Moreover,when comparing G2 and G3,it was found that G2(having the highest textured surface area)displayed the maximum extraction force among all groups,with an increase of 212%when compared to non-textured implants(G1).These results prove that the design and manufacturing technology are effective to promote an im-proved bone-implant bond,aiming the development of orthopedic implants.展开更多
基金FCT(Fundação para a Ciência e a Tecnologia)through the grant SFRH/BD/141056/2018the project PTDC/EME-EME/1442/2020 and under the national support to R&D units grant,through the reference projects UIDB/04436/2020 and UIDP/04436/2020the scope of the project CICECO-Aveiro Institute of Materials,UIDB/50011/2020,UIDP/50011/2020&LA/P/0006/2020,financed by national funds through the FCT/MEC(PIDDAC).
文摘Significant advancements in various research and technological fields have contributed to remarkable findings on the physiological dynamics of the human body.Tomore closely mimic the complex physiological environment,research has moved from two-dimensional(2D)culture systems to more sophisticated three-dimensional(3D)dynamic cultures.Unlike bioreactors or microfluidic-based culture models,cells are typically seeded on polymeric substrates or incorporated into 3D constructs which are mechanically stimulated to investigate cell response to mechanical stresses,such as tensile or compressive.This review focuses on the working principles of mechanical stimulation devices currently available on the market or custom-built by research groups or protected by patents and highlights the main features still open to improvement.These are the features which could be focused on to perform,in the future,more reliable and accurate mechanobiology studies.
基金supported by FTC through the projects PTDC/EMS-TEC/5422/2014 and EXCL/EMS-TEC/ 0460/2012the grant SFRH/BPD/112111/2015+1 种基金supported by FCT with the reference project UID/EEA/04436/2013by FEDER funds through the COMPETE 2020-Programa Operacional Competitividade e Internacionalizacao (POCI) with the reference project POCI-01-0145FEDER-006941.
基金supported by FCT through the grants (No. SFRH/BD/140191/2018)the project No. PTDC/EME-EME/1442/2020 (Add2Mech Bio)+2 种基金by the project No. PTDC/EME-EME/30498/2017 (Fun Imp)also funded by National funds, through the Foundation for Science and Technology (FCT) (project Nos. UIDB/50026/2020 and UIDP/50026/2020)supported by FCT national funds, under the national support to R&D units grant, through the reference projects (Nos. UIDB/04436/2020 and UIDP/04436/2020)
文摘Laser surface texturing is a versatile approach for manufacturing implants with suitable surfaces for os-seointegration.This work explores the use of laser to fabricate NiTi textured implants,testing two dif-ferent groove-based designs.Their performance was evaluated in vivo through implantation in Sprague Dawley rats’femur,being then analyzed after 4 and 12 weeks of implantation.Push-out experiments and histological characterization allowed to assess bone-implant bond and osseointegration and to compare the laser textured solutions with non-textured NiTi.Histology showed that,at 4 weeks of implantation,mainly immature woven bone was present whilst at 12 weeks a more mature bone had developed.Con-sidering the largest implantation time(12 weeks),results showed extraction forces considerably higher for textured implants(G2 and G3).Moreover,when comparing G2 and G3,it was found that G2(having the highest textured surface area)displayed the maximum extraction force among all groups,with an increase of 212%when compared to non-textured implants(G1).These results prove that the design and manufacturing technology are effective to promote an im-proved bone-implant bond,aiming the development of orthopedic implants.
