A full-length cDNA sequence of xyloglucan endotransglycosylase gene （XET）, abundantly expressed in the cambium of Anthocephalus chinensis was cloned by conserved PCR, rapid-amplification of cDNA ends and by chromoso...A full-length cDNA sequence of xyloglucan endotransglycosylase gene （XET）, abundantly expressed in the cambium of Anthocephalus chinensis was cloned by conserved PCR, rapid-amplification of cDNA ends and by chromosome walking. Analytical results of the DNA sequence show that a 912 bp complete open reading frame （ORF） encoded a 303-amino acid protein was in the 1205 bp full cDNA sequence. The deduced amino acid sequence of AcXET, which contained the conserved specific EIDFE catalytic site sequence to XETs was homologous to the other known XET proteins. In order to study the gene function of AcXET and obtain transgenic plants, a plant expression vector pBIAcXET was constructed by recombinating the AcXET fragment from the cloning vector pMD19AcXET and the binary vector pBI121 between the XbaI and SmaI sites. The fragment ofAcXET gene was inserted between the CaMV 35S promotor and the coding region of the GUS gene in pBI121. The identification results show that the plant expression binary vector pBIAcXET was constructed successfully. These results lay the foundation for studying the molecular mechanism ofAcXET gene during wood formation.展开更多
Both cDNA and DNA clones of PtDof1 （GenBank Accession No. FJ402844 and FJ402845） were isolated from plants grown in tissue culture ofPopulus tornentosa. The DNA sequence is 1597 bp including two exons and one intron...Both cDNA and DNA clones of PtDof1 （GenBank Accession No. FJ402844 and FJ402845） were isolated from plants grown in tissue culture ofPopulus tornentosa. The DNA sequence is 1597 bp including two exons and one intron. The cDNA is 969 bp in length with a 765 bp open reading frame which is capable of encoding 255 amino acids. The deduced amino acids sequence of the PtDofl protein shares 65%, 56% and 55% identity with Vitis vinifera （CAO48618）, Nicotiana tabacum （CAA08755） and Glycine max （ABI 16022） Dof protein by blast analysis in GenBank. Phylogenic analysis suggests PtDof1 gene could belong to the Dofgene family. PtDofl protein contains an unusual conserved single zinc finger with the pattern of C-X2-C-X21-C-X2-C, which may play a functional role in tissue-specific expression and possibly the auxin response of endogenous plant genes.展开更多
全基因组关联分析(genome-wide association studies,GWAS)是近几年发展起来的解析人类、动物或植物表型多样性遗传基础的有效分析手段。GWAS具有高通量、高精度和高速度等显著优点,其在林木遗传育种中的作用日益凸显。本文从种质材料...全基因组关联分析(genome-wide association studies,GWAS)是近几年发展起来的解析人类、动物或植物表型多样性遗传基础的有效分析手段。GWAS具有高通量、高精度和高速度等显著优点,其在林木遗传育种中的作用日益凸显。本文从种质材料、目标性状的选择和表型鉴定、全基因组SNP位点的获取、群体结构、连锁不平衡分析以及关联作图与候选基因发掘等方面对GWAS在林木育种中的应用进行了综述,并提出后续研究展望。以期为进一步利用GWAS技术进行林木育种中各种性状遗传基础的研究提供参考。展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 30901158)the Key Project of Chinese Ministry of Education (Grant No. 104243)
文摘A full-length cDNA sequence of xyloglucan endotransglycosylase gene （XET）, abundantly expressed in the cambium of Anthocephalus chinensis was cloned by conserved PCR, rapid-amplification of cDNA ends and by chromosome walking. Analytical results of the DNA sequence show that a 912 bp complete open reading frame （ORF） encoded a 303-amino acid protein was in the 1205 bp full cDNA sequence. The deduced amino acid sequence of AcXET, which contained the conserved specific EIDFE catalytic site sequence to XETs was homologous to the other known XET proteins. In order to study the gene function of AcXET and obtain transgenic plants, a plant expression vector pBIAcXET was constructed by recombinating the AcXET fragment from the cloning vector pMD19AcXET and the binary vector pBI121 between the XbaI and SmaI sites. The fragment ofAcXET gene was inserted between the CaMV 35S promotor and the coding region of the GUS gene in pBI121. The identification results show that the plant expression binary vector pBIAcXET was constructed successfully. These results lay the foundation for studying the molecular mechanism ofAcXET gene during wood formation.
基金supported by the National Natural Science Foundation of China (Grant No. 30271097)
文摘Both cDNA and DNA clones of PtDof1 （GenBank Accession No. FJ402844 and FJ402845） were isolated from plants grown in tissue culture ofPopulus tornentosa. The DNA sequence is 1597 bp including two exons and one intron. The cDNA is 969 bp in length with a 765 bp open reading frame which is capable of encoding 255 amino acids. The deduced amino acids sequence of the PtDofl protein shares 65%, 56% and 55% identity with Vitis vinifera （CAO48618）, Nicotiana tabacum （CAA08755） and Glycine max （ABI 16022） Dof protein by blast analysis in GenBank. Phylogenic analysis suggests PtDof1 gene could belong to the Dofgene family. PtDofl protein contains an unusual conserved single zinc finger with the pattern of C-X2-C-X21-C-X2-C, which may play a functional role in tissue-specific expression and possibly the auxin response of endogenous plant genes.
文摘全基因组关联分析(genome-wide association studies,GWAS)是近几年发展起来的解析人类、动物或植物表型多样性遗传基础的有效分析手段。GWAS具有高通量、高精度和高速度等显著优点,其在林木遗传育种中的作用日益凸显。本文从种质材料、目标性状的选择和表型鉴定、全基因组SNP位点的获取、群体结构、连锁不平衡分析以及关联作图与候选基因发掘等方面对GWAS在林木育种中的应用进行了综述,并提出后续研究展望。以期为进一步利用GWAS技术进行林木育种中各种性状遗传基础的研究提供参考。
