MicroRNAs(miRNAs)are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses.However,it remains unknown whether miRNAs confer the resis...MicroRNAs(miRNAs)are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses.However,it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in rice.To investigate whether miRNAs regulate the resistance to R.solani,we constructed 12 small RNA libraries from susceptible and resistant rice cultivars treated with water/pathogen at 5 h post inoculation(hpi),10 hpi and 20 hpi,respectively.By taking the advantage of next-generation sequencing,we totally collected 400–450 known miRNAs and 450–620 novel miRNAs from the libraries.Expression analysis of miRNAs demonstrated different patterns for known and novel miRNAs upon R.solani challenge.Thirty-four miRNA families were identified to be expressed specifically in rice,and most of them were involved in plant disease resistance.A particular Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis result revealed that a great majority of target genes of regulated miRNAs belonged to the pathway of plant-pathogen interaction.Moreover,miR444b.2,miR531a,mir1861i,novel_miR1956 and novel_miR135 conferred response to R.solani infection confirmed by Northern blot.Our global understanding of miRNA profiling revealed that the regulation of miRNAs may be implicated in the control of rice immunity to R.solani.Analysis of the expression of miRNAs will offer the community with a direction to generate appropriate strategies for controlling rice sheath blight disease.展开更多
Tocopherols synthesized exclusively by photosynthetic organisms are major antioxidants in biomembranes.In plants,tocopherol cyclase(TC/VTE1) catalyzes the conversion of 2,3-dimethyl-5-phytyl-1,4-benzoquinone(DMPBQ) to...Tocopherols synthesized exclusively by photosynthetic organisms are major antioxidants in biomembranes.In plants,tocopherol cyclase(TC/VTE1) catalyzes the conversion of 2,3-dimethyl-5-phytyl-1,4-benzoquinone(DMPBQ) to γ-tocopherol.In the present study,OsVTE1,which encodes a rice tocopherol cyclase ortholog,was cloned and characterized.OsVTE1 was induced significantly by abiotic stresses such as high salt,H2O2,drought,cold and by the plant hormones ABA and salicylic acid.The tissue-specific expression pattern and OsVTE1-promoter GUS activity assay showed that OsVTE1 was mainly expressed in the leaf,and also could be detected in the root,stem and panicle.Compared with control plants,transgenic plants with OsVTE1 RNA interference(OsVTE1-RNAi) were more sensitive to salt stress whereas,in contrast,transgenic plants overexpressing OsVTE1(OsVTE1-OX) showed higher tolerance to salt stress.The DAB in vivo staining showed that OsVTE1-OX plants accumulated less H2O2 than did control plants.展开更多
基金the National Key Research and Development Program of China(Grant No.2016YFD0100601)National Natural Science Foundation of China(Grant No.31672013)+2 种基金FOK YING TUNG Education Foundation(Grant No.151026)High Talent Project of Yangzhou UniversityPriority Academic Program Development of Jiangsu Higher Education Institutions.
文摘MicroRNAs(miRNAs)are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses.However,it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in rice.To investigate whether miRNAs regulate the resistance to R.solani,we constructed 12 small RNA libraries from susceptible and resistant rice cultivars treated with water/pathogen at 5 h post inoculation(hpi),10 hpi and 20 hpi,respectively.By taking the advantage of next-generation sequencing,we totally collected 400–450 known miRNAs and 450–620 novel miRNAs from the libraries.Expression analysis of miRNAs demonstrated different patterns for known and novel miRNAs upon R.solani challenge.Thirty-four miRNA families were identified to be expressed specifically in rice,and most of them were involved in plant disease resistance.A particular Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis result revealed that a great majority of target genes of regulated miRNAs belonged to the pathway of plant-pathogen interaction.Moreover,miR444b.2,miR531a,mir1861i,novel_miR1956 and novel_miR135 conferred response to R.solani infection confirmed by Northern blot.Our global understanding of miRNA profiling revealed that the regulation of miRNAs may be implicated in the control of rice immunity to R.solani.Analysis of the expression of miRNAs will offer the community with a direction to generate appropriate strategies for controlling rice sheath blight disease.
基金supported by the National Basic Research Program of China (Grant No. 2006CB100102)National High-Tech Project (Grant No. 2006AA10Z18201)
文摘Tocopherols synthesized exclusively by photosynthetic organisms are major antioxidants in biomembranes.In plants,tocopherol cyclase(TC/VTE1) catalyzes the conversion of 2,3-dimethyl-5-phytyl-1,4-benzoquinone(DMPBQ) to γ-tocopherol.In the present study,OsVTE1,which encodes a rice tocopherol cyclase ortholog,was cloned and characterized.OsVTE1 was induced significantly by abiotic stresses such as high salt,H2O2,drought,cold and by the plant hormones ABA and salicylic acid.The tissue-specific expression pattern and OsVTE1-promoter GUS activity assay showed that OsVTE1 was mainly expressed in the leaf,and also could be detected in the root,stem and panicle.Compared with control plants,transgenic plants with OsVTE1 RNA interference(OsVTE1-RNAi) were more sensitive to salt stress whereas,in contrast,transgenic plants overexpressing OsVTE1(OsVTE1-OX) showed higher tolerance to salt stress.The DAB in vivo staining showed that OsVTE1-OX plants accumulated less H2O2 than did control plants.
